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铁皮石斛多糖的提取工艺及对小鼠急性肺损伤改善作用的研究
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| 篇名： | 铁皮石斛多糖的提取工艺及对小鼠急性肺损伤改善作用的研究 |
| TITLE： | Study on the extraction of polysaccharides from Dendrobium officinale and its improvement effect on acute lung injury in mice |
| 摘要： | 目的 优化铁皮石斛多糖的提取工艺，初步研究其对小鼠急性肺损伤（ALI）的改善作用。方法以铁皮石斛为原料，采用超声辅助热水浸渍法提取铁皮石斛多糖；以铁皮石斛多糖提取率为响应值，通过单因素实验和Box-Behnken响应面法优化料液比、提取时间、提取温度。通过脂多糖诱导建立ALI小鼠模型，以醋酸泼尼松（5mg/kg）为阳性对照，考察低、中、高剂量铁皮石斛多糖（50、100、200mg/kg）对模型小鼠肺湿/干重比和肺组织病理形态（苏木精-伊红染色、Masson染色）的影响。结果铁皮石斛多糖的最优提取工艺为料液比1∶25（g/mL），提取时间1h，提取温度58℃；该工艺所得铁皮石斛多糖的平均提取率为37.75%（RSD＝1.12%，n＝3），与预测值（38.63%）的相对误差为2.28%。与模型组比较，阳性对照组和铁皮石斛多糖各剂量组小鼠的肺湿/干重比均显著降低（P＜0.05或P＜0.01），肺组织病理变化（肺泡结构严重破坏，肺泡间隔明显增宽，大量炎症细胞浸润及成纤维细胞增生）均有不同程度缓解。结论优化的铁皮石斛多糖提取工艺可行，且所得的铁皮石斛多糖对小鼠ALI有一定的改善作用。 |
| ABSTRACT： | OBJECTIVE To optimize the extraction process of polysaccharides from Dendrobium officinale， and preliminarily study its effect on acute lung injury （ALI） in mice. METHODS Using D. officinale as raw material， the polysaccharides were extracted from D. officinale by ultrasonic-assisted hot water immersion. Using the extraction rate of D. officinale polysaccharides as response value， the single-factor experiments and Box-Behnken response surface method were used to optimize the ratio of material to liquid， extraction time and extraction temperature. ALI mice were induced by lipopolysaccharide. Using prednisone acetate （5 mg/kg） as the positive control， the effects on the mass ratio of wet and dry lung and pathological changes of lung tissue （HE staining and Masson staining） of low-dose， medium-dose and high-dose D. officinale polysaccharides （50，100，200 mg/kg） were investigated. RESULTS The optimal extraction technology of D. officinale polysaccharides was as follows： the ratio of material to liquid was 1∶25 （g/mL）， the extracting time was 1 h， and the extracting temperature was 58 ℃ . Under these conditions， the average extraction rate of D. officinale polysaccharides was 37.75% （RSD＝1.12%，n＝3）， the relative error of which with predicted value （38.63%） was 2.28%. Compared with the model group， the ratios of wet and dry lung in the positive control group and D. officinale polysaccharides groups were all decreased significantly （P＜0.05 or P＜0.01）， and the pathological changes in lung tissue （severe destruction of alveolar structure， significant widening of alveolar septa， extensive infiltration of inflammatory cells and proliferation of fibroblasts） were alleviated to varying degrees. CONCLUSIONS The optimal extraction process of D. officinale polysaccharides is feasible； the obtained polysaccharide extract has a certain improvement effect on ALI in mice. |
| 期刊： | 2023年第34卷第22期 |
| 作者： | 刘子菁；从静文；程卓；牟琳；吴汐柔；王子豪；杨俊颜；信小兵 |
| AUTHORS： | LIU Zijing，CONG Jingwen，CHENG Zhuo，MU Lin，WU Xirou，WANG Zihao，YANG Junyan，XIN Xiaobing |
| 关键字： | 铁皮石斛多糖；提取工艺；Box-Behnken响应面法；脂多糖；急性肺损伤 |
| KEYWORDS： | polysaccharides from Dendrobium officinale； extraction technology； Box-Behnken response surface method； |
| 阅读数： | 32 次 |
| 本月下载数： | 3 次 |
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