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当归多糖改善先兆流产模型大鼠Th1/Th2失衡及保胎作用
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| 篇名： | 当归多糖改善先兆流产模型大鼠Th1/Th2失衡及保胎作用 |
| TITLE： | Angelica sinensis polysaccharides improve Th1/Th2 imbalance and protect pregnancy in threatened abortion model rats |
| 摘要： | 目的 研究当归多糖通过调节磷脂酰肌醇3激酶（PI3K）/蛋白激酶B（AKT）信号通路对先兆流产模型大鼠的保胎作用及Th1/Th2平衡的影响。方法将妊娠大鼠随机分为对照组、模型组、当归多糖组（200mg/kg）、PI3K/AKT信号通路抑制剂LY294002组（5mg/kg）和当归多糖+LY294002组（200mg/kg当归多糖+5mg/kgLY294002），每组10只。除对照组外其余各组大鼠均灌胃米非司酮（8.3mg/kg）和米索前列醇（100μg/kg）建立先兆流产模型，并灌胃或腹腔注射相应药物。检测大鼠血清中雌激素、孕激素、干扰素γ（IFN-γ）、肿瘤坏死因子α（TNF-α）、白细胞介素4（IL-4）水平；测定大鼠子宫卵巢指数及胚胎死亡率；观察大鼠子宫组织形态；检测大鼠外周血中Th1/Th2平衡以及子宫组织中PI3K/AKT信号通路相关蛋白的表达。结果与对照组比较，模型组大鼠子宫组织出现病理损伤；血清中雌激素、孕激素、IL-4水平，子宫卵巢指数、外周血中Th2细胞比例，子宫组织中p-PI3K/PI3K、p-AKT/AKT比值均显著降低（P＜0.05）；胚胎死亡率、Th1细胞比例、Th1/Th2比值和血清中IFN-γ、TNF-α水平均显著升高（P＜0.05）。与模型组比较，当归多糖组大鼠子宫组织病理损伤减轻，上述指标均显著改善（P＜0.05），且LY294002可减弱当归多糖对模型大鼠的改善作用（P＜0.05）。结论当归多糖可通过激活PI3K/AKT信号通路改善先兆流产模型大鼠Th1/Th2失衡，进而抑制免疫炎症，促使胚胎存活。 |
| ABSTRACT： | OBJECTIVE To study the tocolysis effects of Angelica sinensis polysaccharides on threatened abortion model rats and their impacts on Th1/Th2 balance by regulating the phosphoinositide 3-kinase （PI3K）/protein kinase B （AKT） signaling pathway. METHODS Pregnant rats were randomly grouped into the control group， model group， A. sinensis polysaccharide group （200 mg/kg）， PI3K/AKT signaling pathway inhibitor LY294002 group （5 mg/kg）， and A. sinensis polysaccharide+LY294002 group （200 mg/kg A. sinensis polysaccharide+5 mg/kg LY294002）， with 10 rats in each group. Except for the control group， rats in all other groups were given mifepristone （8.3 mg/kg） and misoprostol （100 μg/kg） intragastrically to establish a threatened abortion model， and intragastric or intraperitoneal injection of corresponding drugs. The serum levels of estrogen， progesterone， interferon-γ （IFN-γ）， tumor necrosis factor-α （TNF-α）， and interleukin-4 （IL-4） in each group of rats were detected， and the uterine ovarian index and embryonic mortality rate of rats in each group were measured； the morphology of uterine tissue in rats was observed in each group； Th1/Th2 balance in peripheral blood of rats as well as the expression of PI3K/AKT signaling pathway-related proteins in the uterine tissues of rats in each group were detected. RESULTS Compared with the control group， the uterine tissue of rats in the model group showed pathological damage； the serum levels of estrogen， progesterone and IL-4， uterine ovarian index， peripheral blood Th2 cell ratio， and the ratios of phosphorylated PI3K （p-PI3K）/PI3K and phosphorylated AKT （p-AKT）/AKT in uterine tissue were all decreased （P＜0.05）； the embryo mortality rate， Th1 cell ratio， Th1/Th2 ratio， and serum levels of IFN-γ and TNF-α were increased （P＜0.05）. Compared with the model group， the pathological damage of uterine tissue in the A. sinensis polysaccharide group was reduced， and the above indexes were all improved significantly （P＜0.05）； LY294002 could weaken the effect of A. sinensis polysaccharide on model rats （P＜0.05）. CONCLUSIONS A. sinensis polysaccharides can improve Th1/Th2 imbalance in threatened abortion model rats by activating the PI3K/AKT signaling pathway， thereby inhibiting immune inflammation， and promoting embryo survival. |
| 期刊： | 2023年第34卷第19期 |
| 作者： | 孙哲；刘莲；张泰魏；王琪；周意园；张昌容；华诏召 |
| AUTHORS： | SUN Zhe，LIU Lian，ZHANG Taiwei，WANG Qi，ZHOU Yiyuan，ZHANG Changrong，HUA Zhaozhao |
| 关键字： | 当归多糖；磷脂酰肌醇3激酶/蛋白激酶B信号通路；先兆流产；保胎作用；Th1/Th2平衡 |
| KEYWORDS： | Angelica sinensis polysaccharides； PI3K/AKT signaling pathway； threatened abortion； tocolysis effect； Th1/Th2 |
| 阅读数： | 69 次 |
| 本月下载数： | 3 次 |
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