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清热化湿方通过上调miRNA-155抑制Wnt/β-catenin信号通路治疗胃癌的作用机制研究
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| 篇名： | 清热化湿方通过上调miRNA-155抑制Wnt/β-catenin信号通路治疗胃癌的作用机制研究 |
| TITLE： | Mechanism study of Qingre huashi decoction in the treatment of gastric cancer by up-regulating miRNA-155 and inhibiting Wnt/β-catenin signaling pathway |
| 摘要： | 目的 研究清热化湿方干预miRNA-155抑制Wnt/β-catenin信号通路治疗胃癌的作用机制。方法将30只裸鼠随机分为模型组、对照组（0.004g/kg顺铂+0.02g/kg氟尿嘧啶）、过表达组和清热化湿方低、中、高剂量组（2.71、5.43、10.86g/kg），每组5只。过表达组接种过表达miRNA-155AGS细胞，其余各组均接种AGS细胞，以复制胃癌荷瘤模型。对照组腹腔注射相应药物，其余各组灌胃相应药物或生理盐水，每天1次，连续3周。测定各组裸鼠的肿瘤组织质量，观察肿瘤组织的病理学形态，检测肿瘤组织中miRNA-155以及Wnt7、β-catenin、T细胞因子4（TCF-4）蛋白和mRNA表达水平。结果与模型组比较，对照组、过表达组及清热化湿方高剂量组裸鼠肿瘤组织质量均显著降低（P＜0.05）；Wnt7、β-catenin、TCF-4mRNA和蛋白表达水平均显著降低（P＜0.05），miRNA-155表达水平均显著升高（P＜0.05）；肿瘤细胞均出现不同程度的排列疏松，核染色较浅，出现坏死灶。结论清热化湿方可通过上调miRNA-155，抑制Wnt/β-catenin信号通路中Wnt7、β-catenin、TCF-4mRNA和蛋白的表达，进而抑制胃癌荷瘤模型裸鼠的瘤体生长。 |
| ABSTRACT： | OBJECTIVE To study the mechanism of Qingre huashi decoction in the treatment of gastric cancer by intervening in miRNA-155 and inhibiting Wnt/β-catenin signaling pathway. METHODS Thirty nude mice were randomly divided into model group， control group （0.004 g/kg cisplatin+0.02 g/kg fluorouracil）， overexpression group， Qingre huashi prescription low-dose， medium-dose and high-dose groups （2.71， 5.43， 10.86 g/kg）， with 5 mice in each group. The overexpression group was inoculated with miRNA-155 AGS cell line， and the other groups were inoculated with AGS cells to induce tumor-bearing gastric cancer model. The control group was given relevant medicine intraperitoneally， and other groups were given relevant medicine or normal saline intragastrically， once a day， for 3 consecutive weeks. The weight of tumor tissue in nude mice was determined； the pathological morphology of tumor tissue was observed； the miRNA-155 expression， mRNA and protein expressions of Wnt7， β-catenin and T- cell factor-4（TCF-4） in tumor tissue were detected. RESULTS Compared with the model group， the tumor weights of nude mice in the control group， the overexpression group and Qingre huashi decoction high-dose group were significantly reduced （P＜0.05）； mRNA and protein expressions of Wnt7， β -catenin and TCF-4 were significantly decreased （P＜0.05）， while miRNA-155 expression was increased significantly （P＜0.05）. Tumor cells exhibited varying degrees of loose arrangement， shallow nuclear staining， and necrotic foci. CONCLUSIONS Qingre huashi decoction can inhibit the protein and mRNA expressions of Wnt7， β-catenin and TCF-4 in Wnt/β-catenin signaling pathway by up-regulating miRNA-155， thus inhibiting the tumor growth of tumor-bearing nude mice. |
| 期刊： | 2023年第34卷第19期 |
| 作者： | 尹硕鑫；敖先伟；李博；张涛；陈远能 |
| AUTHORS： | YIN Shuoxin，AO Xianwei，LI Bo，ZHANG Tao，CHEN Yuanneng |
| 关键字： | 清热化湿方；miRNA-155；胃癌；Wnt7；β-catenin；T细胞因子4 |
| KEYWORDS： | Qingre huashi decoction； miRNA-155； gastric |
| 阅读数： | 51 次 |
| 本月下载数： | 3 次 |
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