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艾纳香中艾纳香素的提取工艺优化及抗菌消炎活性评价
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| 篇名： | 艾纳香中艾纳香素的提取工艺优化及抗菌消炎活性评价 |
| TITLE： | Optimization of extraction process and evaluation of the antibacterial and anti-inflammatory activity of blumeatin from Blumea balsamifera |
| 摘要： | 目的 优化艾纳香中艾纳香素的提取工艺，并评价其抗菌消炎活性。方法利用高效液相色谱法测定艾纳香提取液中艾纳香素的含量。在单因素实验的基础上，以艾纳香素得率为评价指标，以乙醇体积分数、液料比、提取时间为考察因素，采用Box-Behnken响应面法优化艾纳香素的提取工艺并进行验证。使用微量稀释法测定艾纳香素对化脓链球菌、金黄色葡萄球菌、无乳链球菌、变异链球菌、枯草芽孢杆菌和藤黄微球菌的抗菌活性。通过小鼠耳廓肿胀实验和毛细血管通透性实验考察艾纳香素的消炎活性。结果最优提取工艺为：乙醇体积分数90%，液料比15∶1，以80℃回流提取2h；采用该提取工艺的艾纳香素得率为1.97mg/g。艾纳香素对化脓链球菌、金黄色葡萄球菌、无乳链球菌、变异链球菌、枯草芽孢杆菌、藤黄微球菌的最低抑菌浓度分别为50.00、200.00、400.00、400.00、800.00、1600.00μg/mL，对化脓链球菌、金黄色葡萄球菌的最低杀菌浓度分别为400.00、1600.00μg/mL；并能显著抑制二甲苯所致小鼠耳廓肿胀及醋酸所致小鼠毛细血管的通透性（P＜0.05或P＜0.01）。结论优化所得的艾纳香素提取工艺稳定可行；提取的艾纳香素对化脓链球菌具有一定的抗菌活性，且具有良好的消炎活性。 |
| ABSTRACT： | OBJECTIVE To optimize the extraction process of blumeatin from Blumea balsamifera and to evaluate its antibacterial and anti-inflammatory activity. METHODS The content of blumeatin in the extract of B. balsamifera was determined by HPLC. On the basis of the single factor experiment， the extraction technology of blumeatin was optimized by the Box-Behnken response surface method with the volume fraction of ethanol， liquid-solid ratio and extraction time as the factors， using the yield of blumeatin as index. Microdilution method was used to determine the antibacterial activity of blumeatin against Streptococcus pyogenes， Staphylococcus aureus， Streptococcus agalactiae， Streptococcus mutans， Bacillus subtilis and Micrococcus luteus. The anti-inflammatory activity of blumeatin was evaluated by ear swelling test and capillary permeability test in mice. RESULTS The optimal extraction technology was as follows： ethanol concentration of 90%， liquid-material ratio of 15∶1， extraction time of 2 h at 80 ℃； the yield of blumeatin using this extraction process was 1.97 mg/g. The minimum inhibitory concentrations of blumeatin for S. pyogenes， S. aureus， S. agalactiae， S. mutans， B. subtilis and M. luteus were 50.00， 200.00， 400.00， 400.00， 800.00 and 1 600.00 μg/mL， respectively； the minimum bactericidal concentrations of blumeatin for S. pyogenes and S. aureus were 400.00 and 1 600.00 μg/mL， respectively. Blumeatin could significantly inhibit the ear swelling induced by xylene and capillary permeability induced by acetic acid in mice（P＜0.05 or P＜0.01）. CONCLUSIONS The optimized extraction technology of blumeatin is stable and feasible. The extracted blumeatin has a certain antibacterial effect against S. pyogenes and a good anti-inflammatory activity. |
| 期刊： | 2023年第34卷第19期 |
| 作者： | 齐维金；刘瑞秀；潘淑娟；何贤芳；王鸿颖；王鲁 |
| AUTHORS： | QI Weijin，LIU Ruixiu，PAN Shujuan，HE Xianfang，WANG Hongying，WANG Lu |
| 关键字： | 艾纳香；艾纳香素；提取工艺；响应面法；消炎；抗菌 |
| KEYWORDS： | Blumea balsamifera； blumeatin； extraction process； response surface method； anti-inflammatory； antibacterial |
| 阅读数： | 38 次 |
| 本月下载数： | 2 次 |
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