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肝泰舒胶囊的成分鉴别及指标成分含量测定方法建立
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| 篇名： | 肝泰舒胶囊的成分鉴别及指标成分含量测定方法建立 |
| TITLE： | Method establishment of components identification and content determination of index components in Gantaishu capsules |
| 摘要： | 目的 建立分别鉴别肝泰舒胶囊的化学成分以及甘草酸等6种指标成分的含量测定方法。方法采用高效液相-飞行时间质谱技术鉴别肝泰舒胶囊中的化学成分；采用超高效液相-串联质谱技术测定甘草酸等6种指标成分的含量。结果肝泰舒胶囊中共鉴别出41种化学成分。甘草酸、芒果苷、木犀草苷、木香烃内酯、齐墩果酸和小檗碱检测质量浓度的线性范围均为200～10000ng/mL（r均大于0.999）；定量限分别为200、20、10、1、10、0.5ng/mL，检测限分别为100、10、5、0.5、5、0.25ng/mL；精密度、稳定性（24h）、重复性试验的RSD均小于5.0%（n＝6或n＝3）；加样回收率为99.05%～101.08%（RSD均小于2.0%，n＝6）；含量分别为2.42～2.66、0.85～1.16、0.35～0.46、6.18～6.46、0.99～1.29、5.22～5.56mg/g。结论本研究所建立的鉴别和含量测定方法快速、简便，可用于肝泰舒胶囊化学成分的鉴别和指标成分的含量测定。 |
| ABSTRACT： | OBJECTIVE To establish methods to identify the chemical components of Gantaishu capsule， and determine the contents of 6 index components including glycyrrhizic acid. METHODS The chemical components of Gantaishu capsule were determined by HPLC-TOF/MS； the contents of 6 index components including glycyrrhizic acid were determined by UPLC-MS/MS. RESULTS A total of 41 chemical components were identified in Gantaishu capsules. The linear ranges of glycyrrhizic acid， mangiferin， luteolin， costunolide， oleanolic acid and berberine were 200-10 000 ng/mL（r were all greater than 0.999）. The limits of quantification were 200， 20， 10， 1， 10， 0.5 ng/mL， and the limits of detection were 100， 10， 5， 0.5， 5， 0.25 ng/mL， respectively； RSDs of precision， stability （24 h） and reproducibility tests were all less than 5.0% （n＝6 or n＝3）； the recoveries were 99.05%-101.08% （RSD were all less than 2.0%， n＝6）. The contents of them were 2.42-2.66， 0.85-1.16， 0.35-0.46， 6.18- 6.46， 0.99-1.29， 5.22-5.56 mg/g. CONCLUSIONS The established methods for identification and content determination are rapid and simple， and can be used for the identification of chemical components and the content determination of index components in Gantaishu capsule. |
| 期刊： | 2023年第34卷第18期 |
| 作者： | 王晓丽；朱平；周燕妮；鲍蕾蕾 |
| AUTHORS： | WANG Xiaoli，ZHU Ping，ZHOU Yanni，BAO Leilei |
| 关键字： | 肝泰舒胶囊；成分鉴别；含量测定；高效液相-飞行时间质谱技术；超高效液相-串联质谱技术 |
| KEYWORDS： | Gantaishu capsules； component identification； content determination； HPLC-TOF/MS； UPLC-MS/MS |
| 阅读数： | 36 次 |
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