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加味三黄膏治疗大鼠肛门溃疡和肿胀的药效机制研究
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| 篇名： | 加味三黄膏治疗大鼠肛门溃疡和肿胀的药效机制研究 |
| TITLE： | Pharmacodynamic mechanism study on Modified sanhuang ointment in the treatment of anal ulcers and swelling in rats |
| 摘要： | 目的 探究加味三黄膏对大鼠肛门溃疡和肿胀的改善作用机制。方法利用冰醋酸建立大鼠肛门溃疡模型，考察加味三黄膏低、中、高剂量组（185、370、740mg/kg）和西药阳性对照组（复方角菜酸酯乳膏，1g/kg）、中药阳性对照组（马应龙麝香痔疮膏，1g/kg）对模型大鼠体重、肛门溃疡面积和肛门溃疡等级的影响。利用巴豆油建立大鼠直肠肿胀模型，考察加味三黄膏低、中、高剂量组（185、370、740mg/kg）和西药阳性对照组（复方角菜酸酯乳膏，1g/kg）、中药阳性对照组（马应龙麝香痔疮膏，1g/kg）对模型大鼠直肠肛门肿胀率、血清中炎症因子[白细胞介素2（IL-2）、IL-4、肿瘤坏死因子α（TNF-α）]含量、直肠组织病理形态、直肠组织中瞬时受体电位通道V1（TRPV1）和P物质表达以及直肠血管通透性的影响。结果加味三黄膏各剂量均可不同程度升高肛门溃疡模型大鼠体重增加量，减少肛门溃疡面积，降低肛门溃疡等级；其能改善直肠肿胀模型大鼠的直肠组织损伤，降低直肠肛门肿胀率和血清中炎症因子含量、直肠组织中TRPV1和P物质表达以及直肠血管通透性（P＜0.05或P＜0.01），其效果优于西药阳性对照、中药阳性对照。结论加味三黄膏可通过降低炎症因子释放、抑制TRPV1及P物质表达来改善大鼠肛门溃疡和肿胀。 |
| ABSTRACT： | OBJECTIVE To explore the improvement mechanism of Modified sanhuang ointment on anal ulcers and swelling model rats. METHODS The anal ulcer model of the rat was induced by using glacial acetic acid； the effects of Modified sanhuang ointment low-dose， medium-dose and high-dose groups （185， 370， 740 mg/kg）， western medicine positive control group （Compound carraghenates cream， 1 g/kg） and TCM positive control group （Mayinglong shexiang zhichuang ointments， 1 g/kg） on body weight， area of anal ulcer， grade of anal ulcer were investigated. The other groups of rats were used to induce rectal swelling models with croton oil； the effects of Modified sanhuang ointment low-dose， medium-dose and high-dose groups （185， 370， 740 mg/kg）， western medicine positive control group （Compound carraghenates cream， 1 g/kg） and TCM positive control group （Mayinglong shexiang zhichuang ointments， 1 g/kg） on the rate of rectal and anal swelling， serum contents of inflammatory factors [interleukin 2 （IL-2）， IL-4， tumor necrosis factor （TNF-α）]， pathological morphology of rectal tissue， the expression of transient receptor potential channel V1 （TRPV1） and substance P in rectal tissue and rectal vascular permeability were investigated. RESULTS In Modified sanhuang ointment， the increase in body weight was enhanced， and the area of anal ulcers， as well as the grade of anal ulcers in rats with anal ulcer models， were reduced to varying degrees； rectal tissue damage in rectal swelling model rats was improved； the rate of rectal and anal swelling， the serum contents of inflammatory factors， the expressions of TRPV1 and substance P in rectal tissue， and rectal vascular permeability were all decreased （P＜0.05 or P＜0.01）. The effect of Modified sanhuang ointment was better than that of western medicine positive control and TCM positive control.Modified sanhuang ointment can improve anal ulcers and swelling in rats by reducing the release of inflammatory factors， inhibiting the expression of TRPV1 and substance P. |
| 期刊： | 2023年第34卷第18期 |
| 作者： | 张馨月；于倩；李胜楠；杨亦柳；李文杰；陈越；严铭铭 |
| AUTHORS： | ZHANG Xinyue，YU Qian，LI Shengnan，YANG Yiliu，LI Wenjie，CHEN Yue，YAN Mingming |
| 关键字： | 加味三黄膏；痔疮；肿胀；TRPV1；P物质 |
| KEYWORDS： | Modified sanhuang ointment； hemorrhoids； |
| 阅读数： | 65 次 |
| 本月下载数： | 4 次 |
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