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玉屏风颗粒通过调节EMT对Lewis荷瘤小鼠肿瘤转移的影响
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| 篇名： | 玉屏风颗粒通过调节EMT对Lewis荷瘤小鼠肿瘤转移的影响 |
| TITLE： | Effects of Yupingfeng granules on tumor metastasis of Lewis tumor-bearing mice by adjusting the EMT |
| 摘要： | 目的 探讨玉屏风颗粒通过调节上皮-间充质转化（EMT）改善上皮屏障功能并抑制肿瘤转移的作用。方法将30只C57BL/6小鼠按随机数字表法分为正常组（蒸馏水）、模型组（蒸馏水）和玉屏风颗粒组（40g/kg），每组10只。模型组与玉屏风颗粒组小鼠通过右腋皮下接种Lewis肺癌细胞复制小鼠自发肺转移模型。造模成功后，各组小鼠灌胃水/药物，每天1次，连续15d。通过观察/测定小鼠肺组织病理形态、肺组织肿瘤转移灶计数、肿瘤转移灶、癌胚抗原表达来考察玉屏风颗粒对肿瘤转移的影响；通过qRT-PCR法和Westernblot法检测小鼠肺组织中β-连环蛋白（β-catenin）、E-钙黏蛋白（E-cadherin）、波形蛋白（vimentin）mRNA及其蛋白表达来考察可能的作用机制。结果与模型组比较，玉屏风颗粒组小鼠肺表面转移结节总数显著减少（P＜0.05），肺组织大体形态有所恢复，肺组织癌胚抗原表达显著减弱（P＜0.05），且肺组织中E-cadherinmRNA及其蛋白表达水平均显著升高（P＜0.05），vimentin、β-cateninmRNA及其蛋白表达水平均显著降低（P＜0.05）。结论玉屏风颗粒可能是通过调控β-catenin表达来抑制EMT的发生，进而改善小鼠的上皮屏障功能，发挥抑制肿瘤细胞侵袭、迁移的作用。 |
| ABSTRACT： | OBJECTIVE To explore the effects of Yupingfeng granules on the improvement of epithelial barrier function and the inhibition of tumor metastasis by regulating epithelial-mesenchymal transition （EMT）. METHODS Thirty C57BL/6 mice were randomly divided into the normal group （distilled water），model group （distilled water） and Yupingfeng granule group （40 g/kg）， with 10 mice in each group. The model group and Yupingfeng granule group were inoculated with Lewis lung cancer cells subcutaneously in the right armpit to induce the spontaneous lung metastasis model. After modeling，each group was given water/ relevant medicine intragastrically，once a day，for 15 consecutive days. The effects of Yupingfeng granules on tumor metastasis were investigated by observing or determining the pathomorphology of lung tissue，metastatic lesion count on the surface of the lung， tumor metastatic lesion and the expression of carcinoembryonic antigens. qRT-PCR and Western blot assay were used to detect the mRNA and protein expressions of β-catenin，E-cadherin and vimentin in the lung tissue of mice. RESULTS Compared with the model group，the total number of pulmonary metastases on the surface was decreased significantly in Yupingfeng granule group （P＜ 0.05），the general morphology of lung tissue was recovered，and the expression of carcinoembryonic antigen in lung tissue was significantly decreased （P＜0.05）. mRNA and protein expressions of E-cadherin in lung tissue were significantly increased （P＜ 0.05），while mRNA and protein expressions of vimentin and β-catenin were significantly decreased （P＜0.05）. CONCLUSIONS Yupingfeng granules can inhibit EMT by regulating the expression of β-catenin，thus improving epithelial barrier function，and inhibiting the ability of tumor cells to invade and metastasize. |
| 期刊： | 2023年第34卷第17期 |
| 作者： | 林映雪；崔海鹏；王领弟；杨昌硕；庄朋伟 |
| AUTHORS： | LIN Yingxue，CUI Haipeng，WANG Lingdi，YANG Changshuo，ZHUANG Pengwei |
| 关键字： | 玉屏风颗粒；上皮-间充质转化；上皮屏障；肿瘤转移；β-连环蛋白 |
| KEYWORDS： | Yupingfeng granules； epithelial-mesenchymal |
| 阅读数： | 45 次 |
| 本月下载数： | 4 次 |
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