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朱砂根、山豆根及其药对的HPLC指纹图谱建立及5种化学成分的含量测定
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| 篇名： | 朱砂根、山豆根及其药对的HPLC指纹图谱建立及5种化学成分的含量测定 |
| TITLE： | Establishment of HPLC fingerprints for Ardisia crenata，Sophora tonkinensis and their couplet medicines and content determination of 5 chemical components |
| 摘要： | 目的 建立朱砂根、山豆根及其药对的指纹图谱，同时测定其中5种成分的含量。方法以水为溶剂，结合冷冻干燥技术制备朱砂根、山豆根单煎冻干粉和朱砂根-山豆根药对合煎冻干粉样品；采用高效液相色谱（HPLC）法建立3种冻干粉样品的指纹图谱，并同法测定没食子酸等5种成分的含量。结果朱砂根、山豆根单煎冻干粉样品和朱砂根-山豆根药对合煎冻干粉样品分别有5、10、14个共有峰；与对照图谱的相似度均大于0.90；朱砂根-山豆根药对合煎冻干粉样品中，指认3号峰为没食子酸、4号峰为苦参碱、6号峰为氧化苦参碱、8号峰为岩白菜素、14号峰为三叶豆紫檀苷。朱砂根单煎冻干粉样品中，没食子酸、岩白菜素的平均含量分别为0.4993、4.9626mg/g；山豆根单煎冻干粉样品中，苦参碱、氧化苦参碱、三叶豆紫檀苷的平均含量分别为3.0460、2.3366、0.2786mg/g；朱砂根-山豆根药对合煎冻干粉样品中，没食子酸、苦参碱、氧化苦参碱、岩白菜素、三叶豆紫檀苷的平均含量分别为0.5606、2.5487、1.3822、5.9607、0.2791mg/g，转移率为8.87%～513.19%。结论所建立指纹图谱及含量测定方法稳定、可行，可用于朱砂根、山豆根及朱砂根-山豆根药对的质量控制；朱砂根-山豆根药对合煎冻干粉样品中苦参碱、氧化苦参碱的平均含量降低。 |
| ABSTRACT： | OBJECTIVE To establish the fingerprints of Ardisia crenata， Sophora tonkinensis and their couplet medicines， and to determine the contents of five components in them. METHODS Using water as solvent， single lyophilized powder of A. crenata and S. tonkinensis and combined lyophilized powder of their couplet medicines were prepared by combining lyophilization technology. The fingerprints of three lyophilized powder samples were established by using high-performance liquid chromatography （HPLC）， and the contents of 5 kinds of components such as gallic acid were determined simultaneously. RESULTS There were 5， 10 and 14 common peaks in the fingerprints for single lyophilized powder of A. crenata and S. tonkinensis and combined lyophilized powder of their couplet medicines； the similarities of them with the control fingerprints were all greater than 0.90. For combined lyophilized powder of couplet medicines， peak 3 Δ 基金项目 国家重点研发计划项目（No.2018YFC1708100）；贵 州省科技计划项目（No.黔科合基础-ZK〔2022〕一般483，No.黔科合成 was identified as gallic acid， peak 4 as matrine， peak 6 as 果〔2021〕一般137）；贵州省教育厅高等学校科学研究项目（青年项目） oxymatrine， peak 8 as bergenin， and peak 14 as trifolirhizin. In single lyophilized powder of A. crenata， the average contents of gallic acid and bergenin were 0.499 3 and 4.962 6 mg/g， respectively. In single lyophilized powder of S.tonkinensis， the average contents of matrine， oxymatrine and trifolirhizin were 3.046 0， 2.336 6 and 0.278 6 mg/g， respectively. In combined lyophilized powder of couplet medicines， the average contents of gallic acid， matrine， oxymatrine， bergenin and trifolirhizin were 0.560 6， 2.548 7， 1.382 2， 5.960 7 and 0.279 1 mg/g， respectively. The transfer rates were 8.87%-513.19%. CONCLUSIONS The established fingerprint and content determination methods are stable and feasible， and can be used for the quality control of A. crenata and S. tonkinensis and their couplet medicines. The average contents of matrine and oxymatrine in combined lyophilized powder of A. crenata-S. tonkinensis couplet medicines are decreased. |
| 期刊： | 2023年第34卷第16期 |
| 作者： | 陈贇；石慧；俸婷婷；张丽艳；董秀；张金鹤；黄蓓；周英 |
| AUTHORS： | CHEN Yun，SHI Hui，FENG Tingting，ZHANG Liyan，DONG Xiu，ZHANG Jinhe，HUANG Bei，ZHOU Ying |
| 关键字： | 朱砂根；山豆根；药对；指纹图谱；含量测定；高效液相色谱法 |
| KEYWORDS： | Ardisia crenata； Sophora tonkinensis； couplet medicines； fingerprint； content determination； high-performance |
| 阅读数： | 64 次 |
| 本月下载数： | 3 次 |
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