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UPLC-QAMS法同时测定连翘花中5种成分的含量
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| 篇名： | UPLC-QAMS法同时测定连翘花中5种成分的含量 |
| TITLE： | Simultaneous determination of five components in Forsythia suspensa flower with UPLC-QAMS |
| 摘要： | 目的 建立同时测定连翘花中芦丁、连翘酯苷A、（+）-松脂素-4-O-β-D-吡喃葡萄糖苷、连翘苷、连翘脂素5种成分的方法。方法采用超高效液相色谱（UPLC）法进行检测。色谱柱为ACQUITYUPLCHSST3C18；流动相为乙腈-0.1%磷酸溶液（梯度洗脱）；流速为0.3mL/min；检测波长为275nm（0～8min）、330nm（8～10.5min）、275nm（10.5～32min）；柱温为25℃；进样量为1μL。以芦丁为参照物，采用一测多评（QAMS）法计算各成分含量，并与外标法进行比较。结果QAMS法所得连翘花中连翘酯苷A、（+）-松脂素-4-O-β-D-吡喃葡萄糖苷、连翘苷、连翘脂素的含量分别为7.472～7.671、2.919～2.986、1.439～1.486、1.523～1.566mg/g，外标法测定结果为7.454～7.664、2.913～2.996、1.444～1.484、1.519～1.562mg/g，两种方法测定结果无明显差异，相对偏差＜1.0%。结论本研究成功建立了UPLC-QAMS法同时测定连翘花中5种成分的含量，且该方法与外标法所得结果无明显差异，可用于连翘花的质量控制。 |
| ABSTRACT： | OBJECTIVE To establish the methods for simultaneous determination of rutin， forsythiaside A， （+）-pinoresinol-4- O-β-D-glucopyranoside， forsythin and forsythigenin in Forsythia suspensa flower. METHODS UPLC method was adopted. The determination was performed on ACQUITY UPLC HSS T3 C18 column with mobile phase consisted of acetonitrile-0.1% phosphoric acid solution （gradient elution） at the flow rate of 0.3 mL/min. The detection wavelengths were set at 275 nm （0-8 min），330 nm （8-10.5 min），275 nm （10.5-32 min）， respectively. The column temperature was 25 ℃， and sample size was 1 μL. Taking rutin as reference， the content of each component was determined by quantitative analysis of multi-components by single-marker （QAMS） method， and then compared with external standard method. RESULTS The contents of forsythiaside A， （+）-pinoresinol-4-O-β-D- glucopyranoside， forsythin and forsythigenin by QAMS were 7.472-7.671， 2.919-2.986， 1.439-1.486， 1.523-1.566 mg/g； the results obtained by the external standard method were 7.454-7.664， 2.913-2.996， 1.444-1.484， 1.519-1.562 mg/g， respectively. There was no significant difference in the measurement results between the two methods， with a relative deviation less than 1.0%. CONCLUSIONS This study successfully establishes the UPLC-QAMS method for simultaneous determination of five components in F. suspensa flower， and the results obtained by this method are not significantly different from those obtained by the external standard method. It can be used for quality control of F. suspensa flower. |
| 期刊： | 2023年第34卷第15期 |
| 作者： | 倪素丽；董岳峰 |
| AUTHORS： | NI Suli，DONG Yuefeng |
| 关键字： | 超高效液相色谱；一测多评法；连翘花；芦丁；连翘酯苷A；连翘苷；连翘脂素；（+）-松脂素-4-O-β-D-吡喃葡萄糖苷 |
| KEYWORDS： | UPLC； quantitative analysis of multi-components by single-marker method； Forsythia suspensa flower； rutin； |
| 阅读数： | 49 次 |
| 本月下载数： | 2 次 |
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