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齿痛消炎灵颗粒中5种成分在大鼠体内的药动学研究
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| 篇名： | 齿痛消炎灵颗粒中5种成分在大鼠体内的药动学研究 |
| TITLE： | Pharmacokinetic study of five ingredients from Chitong xiaoyanling granules in rats |
| 摘要： | 目的 研究齿痛消炎灵颗粒中芍药苷、橙皮苷、柚皮素、芒柄花素和甘草次酸5种成分在大鼠体内的药动学。方法取6只雄性SD大鼠，禁食不禁水12h后一次性灌胃齿痛消炎灵颗粒（5.0g/kg），分别于给药后的不同时间点经眼内眦取血。血浆样品经乙腈沉淀蛋白预处理后（磺胺甲噁唑为内标），采用液相色谱-串联质谱（LC-MS/MS）法检测血浆中5种成分的浓度；采用DAS3.0软件，计算各成分的药动学参数。结果大鼠灌胃齿痛消炎灵颗粒后，5种成分中芒柄花素的tmax、t1/2均最短（分别为0.25、0.39h）；柚皮素和甘草次酸的tmax较短，但t1/2较长，且柚皮素的药-时曲线出现了双峰现象；与其他3个成分相比，芍药苷和橙皮苷的cmax、AUC与体外成分含量高低不成正比。结论齿痛消炎灵颗粒中芒柄花素、柚皮素、甘草次酸均在大鼠体内迅速吸收，芍药苷、橙皮苷均吸收较慢。 |
| ABSTRACT： | OBJECTIVE To study the pharmacokinetics of paeoniflorin， hesperidin， naringenin， formononetin and enoxolone from Chitong xiaoyanling granules in rats. METHODS Six male SD rats fasted but not deprived of water for 12 h were given Chitong xiaoyanling granules （5.0 g/kg） intragastrically at one time. Blood was collected from inner canthus of rats at different time points after administration. Plasma samples were pretreated with acetonitrile precipitated protein （sulfamethoxazole as internal standard）， and the concentrations of 5 ingredients in plasma were determined by liquid chromatography-tandem mass spectrometry （LC-MS/MS）. The pharmacokinetic parameters of each ingredient were calculated using DAS 3.0 software. RESULTS After intragastric administration of Chitong xiaoyanling granules， tmax and t1/2 of formononetin were the shortest among 5 ingredients （0.25 h and 0.39 h， respectively）. For naringenin and enoxolone， the tmax was shorter， but t1/2 was longer， and there was an obvious double-peak phenomenon in the drug-time curve of naringenin. Compared with the other three components， cmax and AUC of paeoniflorin and hesperidin were not directly proportional to the content of in vitro components. CONCLUSIONS Formononetin， naringin and enoxolone in Chitong xiaoyanling granules were absorbed rapidly in rats， while paeoniflorin and hesperidin were absorbed slowly. |
| 期刊： | 2023年第34卷第15期 |
| 作者： | 张子建；张婉婉；张洁茹；王伟然；张炜曼；王春英 |
| AUTHORS： | ZHANG Zijian，ZHANG Wanwan，ZHANG Jieru，WANG Weiran，ZHANG Weiman，WANG Chunying |
| 关键字： | 齿痛消炎灵颗粒；大鼠；药动学；液相色谱-串联质谱 |
| KEYWORDS： | Chitong xiaoyanling granules； rat； pharmacokinetics； LC-MS/MS |
| 阅读数： | 58 次 |
| 本月下载数： | 2 次 |
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