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补阳还五汤对特发性肺纤维化模型大鼠的改善作用机制
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| 篇名： | 补阳还五汤对特发性肺纤维化模型大鼠的改善作用机制 |
| TITLE： | Improving mechanism of Buyang huanwu decoction on idiopathic pulmonary fibrosis model rats |
| 摘要： | 目的 研究补阳还五汤（BYHW）对特发性肺纤维化模型大鼠的改善作用机制。方法将96只Wistar大鼠随机分成空白组、模型组、阳性对照组和BYHW低、中、高剂量组，每组16只。除空白组外，其余各组大鼠采用气管滴注博来霉素（5mg/kg）方法造模。造模当天开始给药，空白组和模型组大鼠按10mL/kg灌胃生理盐水，阳性对照组大鼠按5mg/kg灌胃地塞米松溶液，BYHW低、中、高剂量组大鼠按2.5、5、10g/kg（以生药量计）灌胃药液，每天1次，连续给药28d。测量各组大鼠造模第0、7、14、21、28天的体重；动态可视化观察肺细静脉内黏附白细胞数量；检测血清中肿瘤坏死因子α（TNF-α）、白细胞介素6（IL-6）含量，肺组织中TNF-α、IL-6、羟脯胺酸（HYP）、转化生长因子β（1TGF-β1）含量，肺组织中紧密连接蛋白1（ZO-1）蛋白表达；观察肺组织的病理形态学变化。结果与模型组比较，补阳还五汤高、中剂量组和阳性对照组大鼠造模第14、21、28天时体重均显著升高，肺细静脉内黏附白细胞数量均显著减少，血清和肺组织中TNF-α（BYHW中剂量组血清除外）、IL-6含量以及肺组织中HYP、TGF-β1含量均显著降低，肺组织中ZO-1蛋白表达均显著升高（P＜0.05或P＜0.01）；肺组织病理变化均不同程度改善。结论BYHW可能从改善肺微循环的白细胞黏附、抗炎、抗纤维化等方面发挥抗肺纤维化的作用。 |
| ABSTRACT： | OBJECTIVE To study the improving mechanism of Buyang huanwu decoction （BYHW） on idiopathic pulmonary fibrosis （IPF） model rats. METHODS Ninety-six Wistar rats were randomly divided into blank group， model group， positive control group， and BYHW low-dose， middle-dose and high-dose groups， with 16 rats in each group. Except for the blank group， the IPF model was induced in other groups by intratracheal instillation of bleomycin （5 mg/kg）. Starting from the day of modeling， the blank group and model group were given normal saline （10 mL/kg） intragastrically， while the rats in the positive control group were given dexamethasone solution （5 mg/kg） intragastrically， BYHW low-dose， middle-dose and high-dose groups were treated with BYHW （2.5， 5， 10 g/kg， by crude drug） intragastrically， once a day， for 28 consecutive days. The body mass of rats in each group was measured on days 0， 7， 14， 21 and 28 after modeling. The number of adherent white blood cells in pulmonary veins was observed by a dynamic visualization system. The contents of TNF-α and IL-6 in serum and TNF-α， IL-6， HYP and TGF-β1 in lung tissue were detected； the protein expression of ZO-1 was also detected. The pathomorphological changes in lung tissue were observed. RESULTS Compared with the model group， the body weight of rats all increased significantly in BYHW high-dose and middle-dose groups， positive control group， while the number of adherent white blood cells in pulmonary veins was decreased significantly； the contents of TNF-α （except for serum in BYHW middle-dose group） and IL-6 in serum and lung tissue， the contents of HYP and TGF-β1 in lung tissue were decreased significantly， while the protein expression of ZO-1 in the lung tissue was increased significantly （P＜0.05 or P＜0.01）. The pathological changes of lung tissue were improved to varying degrees. CONCLUSIONS BYHW may play anti-pulmonary fibrosis role by improving leukocyte adhesion， anti-inflammatory， anti-fibrosis， and other aspects of pulmonary microcirculation. |
| 期刊： | 2023年第34卷第14期 |
| 作者： | 刘必旺；赵换 |
| AUTHORS： | LIU Biwang，ZHAO Huan |
| 关键字： | 补阳还五汤；肺纤维化；微循环；紧密连接蛋白1 |
| KEYWORDS： | Buyang huanwu decoction； pulmonary |
| 阅读数： | 69 次 |
| 本月下载数： | 24 次 |
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