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中风瘀热方对缺血性脑卒中模型大鼠的干预作用及机制
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| 篇名： | 中风瘀热方对缺血性脑卒中模型大鼠的干预作用及机制 |
| TITLE： | Intervention effect and mechanism of Zhongfeng yure decoction on ischemic stroke model rats |
| 摘要： | 目的 研究中风瘀热方对缺血性脑卒中模型大鼠的干预作用及机制。方法将85只大鼠随机分为假手术组（生理盐水，n＝15）、模型对照组（生理盐水，n＝18）、尼莫地平片组（阳性对照，10.8mg/kg，n＝18）和中风瘀热方高剂量组（20.52g/kg，n＝17）、中风瘀热方低剂量组（5.13g/kg，n＝17）。预防性连续给药7d（每天1次）后，除假手术组外，其余各组均采用改良线栓法制备大鼠大脑中动脉阻塞（MCAO）模型。造模成功后，各组大鼠继续给药3d。实验期间，观察大鼠一般情况，进行神经功能评分；末次给药后，计算脏器指数，观察大鼠脑梗死面积及脑组织病理形态学变化，检测大鼠脑组织及血清中肿瘤坏死因子α（TNF-α）、白细胞介素6（IL-6）水平及脑组织中胱天蛋白酶3（caspase-3）、磷酸化蛋白激酶B（p-AKT）蛋白的平均光密度值。结果造模3d后，与假手术组比较，模型对照组大鼠神经功能评分，脑指数、脾指数，脑梗死面积占比，脑组织和血清中TNF-α、IL-6水平，脑组织中cas‐pase-3蛋白平均光密度值均显著升高（P＜0.05或P＜0.01）；脑组织出现核固缩、弥散性水肿等病变。与模型对照组比较，各给药组上述指标均有不同程度的改善，其中中风瘀热方高剂量组大鼠脑指数、脾指数，脑梗死面积占比，脑组织和血清中TNF-α水平，脑组织中caspase-3蛋白和p-AKT蛋白的平均光密度值均显著回调（P＜0.05或P＜0.01）。结论中风瘀热方对MCAO模型大鼠有一定的防治作用；其机制可能是通过减少炎症因子TNF-α、IL-6的分泌，下调缺血侧脑组织中caspase-3蛋白表达，上调缺血侧脑组织中p-AKT蛋白表达，从而达到保护神经元的作用。 |
| ABSTRACT： | OBJECTIVE To study the intervention effect and mechanism of Zhongfeng yure decoction on ischemic stroke model rats. METHODS Totally 85 rats were randomly divided into sham operation group （normal saline， n＝15）， model control group （normal saline， n＝18）， Nimodipine tablet group （positive control， 10.8 mg/kg， n＝18）， high-dose group of Zhongfeng yure decoction （20.52 g/kg， n＝17） and low-dose group of Zhongfeng yure decoction （5.13 g/kg， n＝17）， respectively. After 7 days of preventive continuous administration （once a day）， except for the sham operation group， the rats’ middle cerebral artery occlusion （MCAO） model was established by the modified suture method in other groups. After modeling， the rats in each group continued to be administered for 3 days. During experiment， general condition of the rats was observed， and the neurological function score was performed. After the last administration， the organ index was calculated， the cerebral infarction area and pathological changes of brain tissue were observed. The levels of tumor necrosis factor-α （TNF-α） and interleukin 6 （IL-6） in brain tissue and serum， and the average optical density value of caspase-3 and phosphorylated protein kinase B（p-AKT） protein in brain tissue were detected. RESULTS Three days after modeling， compared with sham operation group， the neurological function score， in brain tissue index， spleen tissue index， proportion of cerebral infarction area， the levels of TNF-α and IL-6 in brain tissue and serum， and the average optical density value of caspase-3 protein in brain tissue were significantly increased in the model control group （P＜0.05 or P＜0.01）； karyopyknosis， diffuse edema and other lesions appeared in brain tissue. Compared with the model control group， the above indexes in each administration group were improved to varying degrees. Among them， there were significant regression in brain tissue index， spleen tissue index， proportion of cerebral infarction area， TNF-α level in brain tissue and serum， and the average optical density values of caspase-3 protein and p-AKT protein in brain tissue of rats in high-dose group of Zhongfeng yure decoction （P＜0.05 or P＜0.01）. CONCLUSIONS Zhongfeng yure decoction has a certain intervention and therapeutic effect on MCAO model rats. The mechanism may be to reduce the secretion of inflammatory factors TNF-α and IL-6， down-regulate the expression of caspase-3 protein in ischemic brain tissue， up-regulate the expression of p-AKT protein， so as to protect the neurons. |
| 期刊： | 2023年第34卷第13期 |
| 作者： | 李琬；刘炳周；鞠建明；彭蕴茹；王云 |
| AUTHORS： | LI Wan，LIU Bingzhou，JU Jianming，PENG Yunru，WANG Yun |
| 关键字： | 中风瘀热方；缺血性脑卒中；大脑中动脉阻塞模型；胱天蛋白酶3；磷酸化蛋白激酶B；大鼠 |
| KEYWORDS： | Zhongfeng yure decoction； ischemic stroke； middle cerebral artery occlusion model； caspase-3； phosphorylated |
| 阅读数： | 97 次 |
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