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不同基原白芷中香豆素类成分的差异分析
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| 篇名： | 不同基原白芷中香豆素类成分的差异分析 |
| TITLE： | Differential analysis of coumarins in Angelica dahurica from different origins |
| 摘要： | 目的 筛选两种基原白芷（杭白芷与祁白芷）的香豆素类差异成分。方法采用超高效液相色谱-四极杆-静电场轨道阱高分辨质谱联用（UPLC-Q-Exactive-MS/MS）的非靶向代谢组学技术分析6批杭白芷和12批祁白芷的香豆素类成分，运用主成分分析、偏最小二乘法判别分析和正交偏最小二乘法判别分析方法筛选差异成分，再基于差异成分对样品进行聚类分析。结果18批样品中共鉴定出41种香豆素类成分，有23种为差异成分，其中6种差异成分在杭白芷中含量更高，其余17种差异成分在祁白芷中含量更高。异紫花前胡内酯半乳糖苷在杭白芷中含量明显高于祁白芷。基于23种差异成分，可把杭白芷和祁白芷分别聚为一类。结论筛选出的香豆素类差异成分能很好地区分杭白芷与祁白芷，其中异紫花前胡内酯半乳糖苷的贡献最大，可尝试将其用于杭白芷与祁白芷的鉴别。 |
| ABSTRACT： | OBJECTIVE To screen the differential components of coumarins in Angelica dahurica from two origins （A. dahurica cv.‘ Hangbaizhi’； A. dahurica cv.‘ Qibaizhi’）. METHODS Non-targeted metabolomics technique of UPLC-Q-Exactive- MS/MS was used to analyze the coumarins in 6 batches of A. dahurica cv. ‘Hangbaizhi’ and 12 batches of A. dahurica cv. ‘Qibaizhi’. The differential components were screened by principal component analysis， partial least squares discriminant analysis and orthogonal partial least squares discriminant analysis. Cluster analysis was performed on differential components. RESULTS A total of 41 coumarins were identified in 18 batches of samples， in which 23 coumarins were differential components. Therein， 6 differential components were higher in content in A. dahurica cv.‘ Hangbaizhi’， while 17 differential components were higher in content in A. dahurica cv.‘ Qibaizhi’. The content of marmesin galactoside in A. dahurica cv.‘ Hangbaizhi’ was significantly higher than that in A. dahurica cv.‘ Qibaizhi’. Based on 23 differential components， A. dahurica cv.‘ Hangbaizhi’ and A. dahurica cv. ‘Qibaizhi’ could be grouped into one category， respectively. CONCLUSIONS The screened differential components of coumarins can be used to distinguish A. dahurica cv. ‘Hangbaizhi’ from A. dahurica cv. ‘Qibaizhi’， especially marmesin galactoside contributed the most， which can be used to identify A. dahurica cv.‘ Hangbaizhi’ and A. dahurica cv.‘ Qibaizhi’. |
| 期刊： | 2023年第34卷第12期 |
| 作者： | 林颖瑜；杨莹莹；田恩伟 |
| AUTHORS： | LIN Yingyu，YANG Yingying，TIAN Enwei |
| 关键字： | 白芷；非靶向代谢组学；多元统计分析；香豆素；基原鉴定 |
| KEYWORDS： | Angelica dahurica； Non-targeted metabolomics technique； multivariate statistical analysis； coumarin； origin |
| 阅读数： | 139 次 |
| 本月下载数： | 3 次 |
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