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基于多成分定量结合化学计量学评价青黛的质量
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| 篇名： | 基于多成分定量结合化学计量学评价青黛的质量 |
| TITLE： | Quality evaluation of Indigo Naturalis based on multi-component quantification combined with chemometrics |
| 摘要： | 目的 综合评价青黛的质量，为青黛质量控制提供参考。方法采用超高效液相色谱-三重四极杆串联质谱（UPLC-MS/MS）法同时测定不同产地青黛中6个吲哚生物碱（靛蓝、靛玉红、靛红、色胺酮、吲哚、吲哚-3-甲醛）的含量，采用聚类分析、主成分分析、偏最小二乘法-判别分析（PLS-DA）对不同产地的青黛质量进行评价。结果不同产地青黛中靛蓝、靛玉红、靛红、色胺酮、吲哚、吲哚-3-甲醛含量的范围分别为20320.83～26585.01、1327.69～3102.25、141.69～894.50、2.17～5.27、2.14～5.93、1.69～4.34μg/g。聚类分析将不同产地的青黛聚为2类，S1、S2、S4、S6、S7、S9、S10号样品聚为Ⅰ类，S3、S5、S8、S11、S12号样品聚为Ⅱ类。用3个主成分对不同产地青黛进行评价，结果显示，Ⅰ类样品得分较高，质量较优，Ⅱ类样品得分较低，质量较差。PLS-DA结果显示，靛蓝、靛玉红、色胺酮、靛红是体现青黛质量差异的主要物质。结论不同产地的青黛质量差异较大，且同一产地不同批次的青黛质量不稳定。本文所建立的青黛质量评价方法稳定可靠，可为青黛的质量控制提供依据。 |
| ABSTRACT： | OBJECTIVE To evaluate the quality of Indigo Naturalis， and to provide reference for the quality control of Indigo Naturalis. METHODS UPLC-MS/MS method was used to determine the contents of 6 indole alkaloids （indigo， indirubin， isatin， tryptanthrin， indole and indole-3-carboxaldehyde） in Indigo Naturalis from different origins. Cluster analysis， principal component analysis and partial least squares-discriminant analysis （PLS-DA） were used to evaluate the quality of Indigo Naturalis from different origins. RESULTS The contents of indigo， indirubin， isatin， tryptanthrin， indole and indole-3-carboxaldehyde in Indigo Naturalis from different origins were 20 320.83-26 585.01， 1 327.69-3 102.25， 141.69-894.50， 2.17-5.27， 2.14-5.93 and 1.69-4.34 μg/g， respectively. The Indigo Naturalis from different areas were clustered into two categories by cluster analysis. Samples S1， S2， S4， S6， S7， S9 and S10 were clustered into category Ⅰ， and samples S3， S5， S8， S11 and S12 were clustered into category Ⅱ. Indigo Naturalis from different origins was evaluated with 3 principal components. The results showed that category Ⅰ sample scored higher and had better quality， while category Ⅱ sample scored lower and had worse quality. PLS-DA showed that indigo， indirubin， tryptanthrin and isatin were the main substances that reflected the quality difference of Indigo Naturalis. CONCLUSIONS The quality of Indigo Naturalis from different origins is different， and the quality of Indigo Naturalis of different batches from the same area is not stable. The quality evaluation method of Indigo Naturalis established in this paper is stable and reliable， which can provide a basis for the quality control of Indigo Naturalis. |
| 期刊： | 2023年第34卷第08期 |
| 作者： | 孙全；王立娟；唐菱；冷静；傅超美 |
| AUTHORS： | SUN Quan，WANG Lijuan，TANG Ling，LENG Jing，FU Chaomei |
| 关键字： | 青黛；吲哚生物碱；超高效液相色谱-三重四极杆串联质谱；含量测定；化学计量学；质量评价 |
| KEYWORDS： | Indigo Naturalis； indole alkaloid； UPLC-MS/MS； content determination； chemometrics； quality evaluation |
| 阅读数： | 183 次 |
| 本月下载数： | 3 次 |
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