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百合地黄汤多糖提取工艺优化及抗焦虑抑郁药效评价
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| 篇名： | 百合地黄汤多糖提取工艺优化及抗焦虑抑郁药效评价 |
| TITLE： | Optimization of extraction process of polysaccharide from Baihe dihuang decoction and study on its anti-anxiety and anti-depression efficacy |
| 摘要： | 目的 优化百合地黄汤多糖提取工艺并初步评价其抗焦虑抑郁药效。方法在Plackett-Burman实验基础上，以多糖得率与多糖含量的综合评分为评价指标，提取时间、加水量、醇沉浓度为考察因素，结合Box-Behnken响应面法优化百合地黄汤多糖提取工艺并验证。将40只ICR小鼠分为对照组、文法拉辛组[阳性对照，13.5mg/（kg·d）]和百合地黄汤多糖高、低剂量组[5.28、2.64g/（kg·d），以生药量计]，每组10只（雌雄各半）。药物组小鼠灌胃相应药液，对照组小鼠灌胃水10mL/kg，每天1次，连续7d。采用高架十字迷宫实验、旷场实验、强迫游泳实验、悬尾实验评价最优工艺所制提取物对小鼠焦虑抑郁样行为的改善情况，并采用酶联免疫吸附测定法检测其对小鼠脑组织中神经递质水平的影响。结果百合地黄汤多糖的最优提取工艺为加25倍量水，提取1.5h，醇沉浓度70%。3次验证实验的多糖得率、多糖含量平均值分别为33.10%、0.62mg/mg，与预测值（36.14%、0.65mg/mg）的相对偏差分别为8.40%、4.62%（RSD＜2%，n＝3）。所得百合地黄汤多糖提取物能显著增加小鼠进入开放臂的次数比例及开放臂停留时间比例、自主活动总距离及中心区域活动距离，并显著缩短其强迫游泳实验、悬尾实验不动时间（P＜0.05或P＜0.01）；可显著升高小鼠脑组织中5-羟色胺、去甲肾上腺素（百合地黄汤多糖低剂量组除外）、γ氨基丁酸水平，并显著降低谷氨酸（百合地黄汤多糖低剂量组除外）水平（P＜0.05或P＜0.01）。结论优化的百合地黄汤多糖提取工艺稳定、可行；所得多糖提取物具有明显的体内抗焦虑抑郁作用。 |
| ABSTRACT： | OBJECTIVE To optimize the extraction process and to primarily evaluate the anti-anxiety and anti-depression efficacy of polysaccharide from Baihe dihuang decoction. METHODS Based on Plackett-Burman experimental design， using the comprehensive score of yield and content of polysaccharide as indicators， with extraction time， water amount， alcohol precipitation concentration as factors， Box-Behnken response surface methodology was used to optimize the extraction process of polysaccharide from Baihe dihuang decoction； and the validation test was conducted. Forty ICR mice were divided into control group， venlafaxine group [positive control， 13.5 mg/（kg·d）]， Baihe dihuang polysaccharide high-dose and low-dose groups [5.28， 2.64 g/（kg·d），by raw material]， with 10 mice in each group （half male and half female）. Administration groups were given corresponding drug solution intragastrically， and control group was given water 10 mL/kg intragastrically， once a day， for 7 test， forced swimming test and tail suspension test were used to evaluate the effects of the extract prepared by the optimal process on the anxiety-like and depression-like behavior of mice； enzyme-linked immunosorbent assay was used to detect the effects of the extract on the levels of neurotransmitter in cerebral tissue of mice. RESULTS The optimal extraction process of Baihe dihuang decoction was： the water amount of 25 times， extract time of 1.5 hours， and alcohol precipitation concentration of 70%. In 3 times of validation test， the average yield and content of polysaccharide were 33.10% and 0.62 mg/mg， the relative deviations of which from the predicted values （36.14% and 0.65 mg/mg） were 8.40% and 4.62% respectively （RSD＜2%， n＝3）. The polysaccharide extract of Baihe dihuang decoction could effectively increase the percentages of open-arms entry， the percentages of open-arms time， the total distance of voluntary activities and the activity distance in central area， and significantly shortened the immobility time of forced swimming test and tail suspension test （P＜0.05 or P＜0.01）. The polysaccharide extract could significantly increase the levels of 5-hydroxytryptamine， norepinephrine （except for the Baihe dihuang polysaccharide low-dose group） and gamma-aminobutyric acid in cerebral tissue of mice， while significantly decrease the levels of glutamic acid （except for the Baihe dihuang polysaccharide low-dose group） （P＜0.05 or P＜ 0.01）. CONCLUSIONS The optimized extraction process of polysaccharide from Baihe dihuang decoction is stable and feasible， and the obtained polysaccharide extract has obvious anti-anxiety and anti-depression effect in vivo. |
| 期刊： | 2023年第34卷第08期 |
| 作者： | 唐林；赵洪庆；杨蕙；刘检；何怡然；李莉；张水寒 |
| AUTHORS： | TANG Lin，ZHAO Hongqing，YANG Hui，LIU Jian，HE Yiran，LI Li，ZHANG Shuihan |
| 关键字： | 百合地黄汤；多糖；提取工艺；Plackett-Burman设计；Box-Behnken响应面法；抗抑郁；抗焦虑；小鼠 |
| KEYWORDS： | Baihe dihuang decoction； polysaccharide； extraction process； Plackett-Burman design； Box-Behnken response |
| 阅读数： | 505 次 |
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