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多指标成分结合多元统计分析法综合评价黔产黑骨藤质量
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| 篇名： | 多指标成分结合多元统计分析法综合评价黔产黑骨藤质量 |
| TITLE： | Comprehensive quality evaluation of Periploca forrestii produced in Guizhou using multi-index components combined with multivariate statistical analysis |
| 摘要： | 目的 建立多指标成分结合多元统计分析的综合质量评价方法，综合评价黑骨藤药材的质量。方法以贵州不同产地的11批黑骨藤药材为样品，采用高效液相色谱法测定其中新绿原酸、隐绿原酸、绿原酸、原花青素A2、异绿原酸A、异绿原酸C的含量，并采用聚类热图分析、灰色关联度分析（GRA）和熵权逼近理想解排序（TOPSIS）法综合评价其质量。结果含量测定的方法学考察结果均符合相关规定，各待测成分在各自进样量范围内线性关系和准确度均良好。11批样品中绿原酸、隐绿原酸、新绿原酸、原花青素A2、异绿原酸A、异绿原酸C的含量分别为3.650～7.302、0.888～2.575、1.371～2.386、0.947～1.469、0.084～0.169、0.725～1.067mg/g；各成分含量差异显著，其中以绿原酸含量最高，异绿原酸A最低。综合聚类热图、GRA和TOPSIS分析结果显示，样品S5和S10的综合质量相对较好。结论所建立的含量测定方法准确、稳定、简便，结合多元统计分析方法，可用于黑骨藤药材的质量评价。在11批不同产地样品中，以贵州省安顺市西秀区旧州镇和蔡官镇所产样品的质量相对较好。 |
| ABSTRACT： | OBJECTIVE To establish comprehensive quality evaluation method based on multi-index components combined with multivariate statistical analysis， and to comprehensively evaluate the quality of Periploca forrestii. METHODS Taking 11 batches of P. forrestii medicinal materials from different areas in Guizhou as samples， the contents of neochlorogenic acid， cryptochlorogenic acid， chlorogenic acid， procyanidin A2， isochlorogenic acid A and isochlorogenic acid C were determined by HPLC. Clustering heat map analysis， grey correlation analysis（GRA） and technique for order preference by similarity to ideal solution（TOPSIS） were used to evaluate the quality of P. forrestii. RESULTS The results of methodological investigation of content determination were in accordance with the relevant regulations， and the linear relationship and accuracy of each component were good in their respective sampling range. The contents of chlorogenic acid， cryptochlorogenic acid， neochlorogenic acid， procyanidin A2， isochlorogenic acid A and isochlorogenic acid C in 11 batches of samples were 3.650-7.302， 0.888-2.575， 1.371- 2.386， 0.947-1.469， 0.084-0.169 and 0.725-1.067 mg/g， respectively. The content of each component was significantly different， with the highest content of chlorogenic acid and the lowest content of isochlorogenic acid A. The comprehensive results of cluster heat map， GRA and TOPSIS analysis showed that the comprehensive quality of S5 and S10 was relatively good. CONCLUSIONS The established method is accurate， stable and simple. Combined with multivariate statistical analysis method， it can be used for quality evaluation of P. forrestii. The quality of samples from Jiuzhou Town and Caiguan Town of Xixiu District in Anshun City of Guizhou Province are relatively good among 11 different origin samples. |
| 期刊： | 2023年第34卷第07期 |
| 作者： | 曾令琴；何康；郭江涛；刘杰；张永萍；孙宜春；李慧馨 |
| AUTHORS： | ZENG Lingqin，HE Kang，GUO Jiangtao，LIU Jie，ZHANG Yongping，SUN Yichun，LI Huixin |
| 关键字： | 黑骨藤；含量测定；多指标成分；多元统计分析；质量评价 |
| KEYWORDS： | Periploca forrestii； content determination； multi-index component； multivariate statistical analysis； quality |
| 阅读数： | 146 次 |
| 本月下载数： | 2 次 |
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