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火麻仁油改善UC模型大鼠的症状及对肠道菌群的影响
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| 篇名： | 火麻仁油改善UC模型大鼠的症状及对肠道菌群的影响 |
| TITLE： | Effects of Cannabis sativa oil on the symptom improvement and intestinal flora in UC model rats |
| 摘要： | 目的 研究火麻仁油对右旋葡聚糖硫酸钠（DSS）诱导的溃疡性结肠炎（UC）模型大鼠症状的改善作用，并探讨其对大鼠肠道菌群的影响。方法将40只SD大鼠随机分为对照组、模型组、火麻仁油组（1g/kg）和柳氮磺胺吡啶组（阳性对照，300mg/kg），每组10只。对照组和模型组大鼠灌胃0.5%聚山梨酯80，火麻仁油组和柳氮磺胺吡啶组大鼠灌胃相应药液，每天1次，连续10d。从第4天开始，模型组、火麻仁油组和柳氮磺胺吡啶组大鼠连续7d饮用4%DSS溶液以建立UC模型。观察大鼠体质量、疾病活动指数（DAI）评分、结肠长度、结肠质量、结肠单位长度质量、结肠组织病理学变化，检测其血清肿瘤坏死因子α（TNF-α）、白细胞介素6（IL-6）、IL-10含量，通过高通量测序法检测各组大鼠肠道菌群的变化。结果与对照组比较，模型组大鼠的体质量显著降低，结肠长度显著缩短，DAI评分、结肠质量、结肠单位长度质量和血清TNF-α、IL-6含量均显著增加，IL-10含量显著降低（P＜0.05）；结肠组织上皮层发生脱落，炎症细胞浸润且入侵黏膜下层，肠腺紊乱。与模型组比较，火麻仁油组和柳氮磺胺吡啶组大鼠上述指标均显著逆转（P＜0.05），且相关症状均有明显改善。菌群测序结果显示，模型组大鼠的ACE指数和Chao1指数均较对照组显著降低（P＜0.05），火麻仁油组大鼠的Chao1指数较模型组显著升高（P＜0.05）。与对照组比较，模型组有41个菌属发生改变；与模型组比较，火麻仁油能使上述41个菌属中的3个菌属回调至正常状态，包括杜波西菌属、紫杆菌属和别样棒菌属。结论火麻仁油能通过调节肠道菌群多样性，增加有益菌和减少有害菌来改善UC模型大鼠症状。 |
| ABSTRACT： | OBJECTIVE To study the improvement effects of Cannabis sativa oil on the symptoms in dextran sodium sulfate （DSS）-induced ulcerative colitis （UC） model rats， and to investigate its effects on intestinal flora of rats. METHODS Forty SD rats were randomly divided into control group， model group， C. sativa oil group （1 g/kg） and sulfasalazine group （positive control， 300 mg/kg）， with 10 rats in each group. The rats in control group and model group were given 0.5% polysorbate 80 by gavage， and the rats in C. sativa oil group and sulfasalazine group were given corresponding drug solution by gavage once a day for 10 days. From the 4th day， rats in model group， C. sativa oil group and sulfasalazine group were given 4% DSS solution for 7 consecutive days to establish UC model. The body weight， disease activity index （DAI） score， colon length， colon weight， weight per unit length of colon， the pathological changes of colon tissue， and the contents of tumour necrosis factor-α （TNF-α）， interleukin-6 （IL-6） and IL-10 in serum of rats were determined. The changes of intestinal flora in rats were detected by high- throughput sequencing. RESULTS Compared with control group， the body weight and the length of colon were decreased significantly in model group， while DAI score， the weight of colon， weight per unit length of colon， serum contents of TNF-α and IL-6 were increased significantly， and the content of IL-10 was decreased significantly （P＜0.05）； epithelial layer of colon tissue fell off， inflammatory cells infiltrated and invaded the submucosa， and intestinal glands were disordered. Compared with model group， above indexes of C. sativa oil group and sulfasalazine group were reversed significantly （P＜0.05）， and related symptoms were improved significantly. The result of flora sequencing showed that ACE index and Chao1 index of model group were decreased significantly， compared with control group （P＜0.05）； while Chao1 index of C. sativa oil group was increased significantly， compared with model group （P＜0.05）. Compared with control group， 41 genera of bacteria in the model group changed； compared with model group， C. sativa oil could return 3 of the 41 genera to normal state， including Dubosilla， Porphyrobacter and Allobaculum. CONCLUSIONS C. sativa oil can improve the symptoms of UC model rats by regulating the diversity of intestinal flora， increasing beneficial bacteria and decreasing pathogenic bacteria. |
| 期刊： | 2023年第34卷第06期 |
| 作者： | 龚梦鹃；高心悦；简晓敏；邹忠杰 |
| AUTHORS： | GONG Mengjuan，GAO Xinyue，JIAN Xiaomin，ZOU Zhongjie |
| 关键字： | 火麻仁油；溃疡性结肠炎；肠道菌群；高通量测序；大鼠 |
| KEYWORDS： | Cannabis sativa oil； ulcerative colitis； |
| 阅读数： | 193 次 |
| 本月下载数： | 2 次 |
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