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不同煎煮时间桑白皮汤煮散与汤剂的特征图谱及多指标成分含量比较
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| 篇名： | 不同煎煮时间桑白皮汤煮散与汤剂的特征图谱及多指标成分含量比较 |
| TITLE： | Comparison of characteristic chromatogram and the contents of multiple indicator components of Morus alba decoction powder and decoction at different decoction time |
| 摘要： | 目的 比较不同煎煮时间桑白皮汤煮散与汤剂（后文简称为“煮散”和“汤剂”）物质基准的特征图谱与多指标成分含量变化，为桑白皮汤的制剂开发提供实验基础。方法以不同煎煮时间的煮散与汤剂样品为研究对象。利用《中药色谱指纹图谱相似度评价系统（2012版）》软件建立2种样品的高效液相色谱（HPLC）特征图谱，并进行相似度评价；采用HPLC法测定煮散与汤剂样品中桑皮苷A、栀子苷、小檗碱、黄芩苷、槲皮素和木犀草素含量，以各指标成分含量及总含量变化为评价指标比较二者煎煮过程的差异。结果不同煎煮时间煮散和汤剂特征图谱的相似度分别为0.943～1.000、0.975～0.998。煮散中6种指标成分的溶出速率较快且含量较高；煎煮20min时，煮散中各指标成分含量分别为汤剂煎煮50min时的1.1～1.5倍，煮散中各指标成分的总含量为汤剂的1.2倍。结论与汤剂相比，桑白皮汤煮散具有缩短煎煮时间、节约中药资源的优势。本研究结果为“遵古”开发本方剂奠定了基础。 |
| ABSTRACT： | OBJECTIVE To compare characteristic chromatogram and the contents of multiple indicator components of Morus alba decoction powder and decoction at different decoction time， and to provide experimental basis for the development of M. alba decoction. METHODS Taking decoction powder and decoction at different decoction time as subject， HPLC characteristic chromatogram of 2 kinds of samples were established with Similarity Evaluation Software System of TCM Chromatographic Fingerprint （2012 version）， and similarity evaluation was performed. The contents of mulberroside A， geniposide， berberine， baicalin， quercetin and luteolin in decoction powder and decoction were determined by HPLC. The contents of each indicator component and the change of total content were as the evaluation indexes to compare the difference between the two substances during decoction. RESULTS The similarities of characteristic chromatogram of the two substances ranged from 0.943 to 1.000 and 0.975 to 0.998 at different decoction time， respectively. Six indicator components of the decoction powder dissolved faster and had higher contents. The contents of each indicator component in the decoction powder when decocting at 20 minutes was 1.1-1.5 times of the decoction when decocting at 50 min， and the total content in the decoction powder was 1.2 times of the decoction. CONCLUSIONS Compared with decoction， M. alba decoction powder has the advantages of shortening the decoction time and saving traditional Chinese medicine resources. The results of this study lay a research foundation for “Zungu” to develop its preparation. |
| 期刊： | 2023年第34卷第05期 |
| 作者： | 蒋近近；吴磊；朱育凤；孙思；邱明鸣；陆超 |
| AUTHORS： | JIANG Jinjin，WU Lei，ZHU Yufeng，SUN Si，QIU Mingming，LU Chao |
| 关键字： | 桑白皮汤；物质基准；煮散；汤剂；特征图谱；含量测定 |
| KEYWORDS： | Morus alba decoction； material basis； decoction powder； decoction； characteristic chromatogram； content |
| 阅读数： | 136 次 |
| 本月下载数： | 4 次 |
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