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壮药三七姜醇提物及含药血清对脂多糖诱导RAW264.7细胞炎症的抗炎作用及机制
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| 篇名： | 壮药三七姜醇提物及含药血清对脂多糖诱导RAW264.7细胞炎症的抗炎作用及机制 |
| TITLE： | Anti-inflammatory effect and mechanism of the ethanol extract and the drug-containing serum of Zhuang medicine Stahlianthus involucratus on LPS-induced RAW264.7 cell inflammation |
| 摘要： | 目的 研究壮药三七姜醇提物及含药血清对脂多糖（LPS）诱导RAW264.7细胞炎症的抗炎作用及机制。方法将三七姜醇提物（75.35g/kg）或纯净水灌胃大鼠以制备含药血清或空白血清。以RAW264.7细胞为研究对象，将细胞分为正常对照组，LPS组（1μg/mL），三七姜醇提物高、中、低剂量组（50、25、12.5μg/mL），4%或15%空白血清组，4%或15%空白血清+LPS组，4%或15%含药血清组，4%或15%含药血清+LPS组，按相应条件培养24h后，检测各组细胞活力和细胞中一氧化氮（NO）、肿瘤坏死因子α（TNF-α）、白细胞介素1β（IL-1β）、IL-6含量，以及Toll样受体4（TLR4）、核因子κB（NF-κB）mRNA表达水平，一氧化氮合酶（NOS）、环氧化酶2（COX-2）蛋白表达水平。结果各组细胞培养24h后，细胞活力差异无统计学意义。与正常对照组比较，LPS组细胞中NO、TNF-α、IL-1β、IL-6含量，TLR4、NF-κBmRNA表达水平和NOS、COX-2蛋白表达水平均显著升高（P＜0.05）。与4%或15%空白血清组比较，4%或15%空白血清+LPS组细胞中上述指标水平显著升高（P＜0.05）。与LPS组比较，三七姜醇提物各剂量组大鼠上述指标水平均显著降低（P＜0.05）。与4%或15%空白血清+LPS组比较，4%或15%含药血清+LPS组细胞中上述指标水平均显著降低（P＜0.05）。结论三七姜醇提物及含药血清均可明显减轻LPS诱导的细胞炎症反应，其抗炎机制可能与抑制TLR4/NF-κB信号通路活性，下调COX-2、NOS蛋白表达，减少炎症因子释放有关。 |
| ABSTRACT： | OBJECTIVE To study the anti-inflammatory effect and mechanism of the ethanol extract and the drug-containing serum of Zhuang medicine Stahlianthus involucratus on lipopolysaccharide （LPS）-induced RAW264.7 cell inflammation. METHODS The drug-containing serum or blank serum was obtained by intragastrical administration of ethanol extract of S. involucratus （75.35 g/kg） or purified water. Using RAW264.7 cells as objects， RAW264.7 cells were divided into normal control group， LPS group （1 μg/mL）， S. involucratus ethanol extract high-dose， medium-dose and low-dose groups （50， 25， 12.5 μg/mL）， 4% or 15% blank serum groups， 4% or 15% blank serum+LPS groups， 4% or 15% drug-containing serum groups， 4% or 15% drug-containing serum+LPS groups. After culturing for 24 h， cell viability， the contents of nitric oxide tumor necrosis factor α （TNF-α）， interleukinand IL-6 as well as mRNA expressions of Toll-like eceptor 4 （TLR4） and nuclear factor κB （NF- κB） and protein expressions of nitric oxide synthase （NOS） and cyclooxygenase 2 （COX-2） were all detected in each group. 0771-4953513。E-mail：zhuhuagx@163.com RESULTS After culturing for 24 h， there was no statisticalsignificance in the difference of cell viability. Compared with normal control group， the contents of NO， TNF-α， IL-1β and IL-6， mRNA expressions of TLR4 and NF-κB， and protein expressions of NOS and COX-2 were increased significantly in LPS group （P＜0.05）. Compared with 4% or 15% blank serum groups， the levels of above indexes were increased significantly in 4% or 15% blank serum+LPS groups （P＜0.05）. Compared with LPS group， the levels of above indexes were decreased significantly in S. involucratus ethanol extract groups （P＜0.05）. Compared with 4% or 15% blank serum+LPS groups， the levels of above indexes were decreased significantly in 4% or 15% drug-containing serum+LPS groups （P＜0.05）. CONCLUSIONS The ethanol extract and the drug-containing serum of S. involucratus can significantly alleviate LPS-induced inflammatory reaction， the mechanism of which may be associated with inhibiting the activity of TLR4/NF-κB signaling pathway， down-regulating the protein expressions of COX-2 and NOS， and reducing the release of inflammatory factors. |
| 期刊： | 2023年第34卷第05期 |
| 作者： | 梁洁；梁晶春；刘星晨；陈晓思；曹玉嫔；陈俊；柳贤福；李耀华；朱华 |
| AUTHORS： | LIANG Jie， LIANG Jingchun，LIU Xingchen，CHEN Xiaosi，CAO Yupin，CHEN Jun，LIU Xianfu，LI Yaohua，ZHU Hua |
| 关键字： | 三七姜；TLR4/NF-κB信号通路；炎症因子；巨噬细胞 |
| KEYWORDS： | Stahlianthus involucratu； TLR4/NF-κB signaling pathway； inflammatory factors； macrophage |
| 阅读数： | 126 次 |
| 本月下载数： | 3 次 |
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