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硫化氢对心肌成纤维细胞增殖的抑制作用及机制研究
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| 篇名： | 硫化氢对心肌成纤维细胞增殖的抑制作用及机制研究 |
| TITLE： | Study on inhibitory effect and mechanism of hydrogen sulfide on the proliferation of cardiac fibroblasts |
| 摘要： | 目的 研究硫化氢（H2S）对心肌成纤维细胞增殖的抑制作用及机制。方法取新生SD雄性大鼠心脏，采用差速离心法分离心肌成纤维细胞。以硫氢化钠作为H2S的供体，检测H2S对血管紧张素Ⅱ（AngⅡ）诱导心肌成纤维细胞增殖、羟脯氨酸含量以及去乙酰化酶3（SIRT3）蛋白表达的影响。用干扰RNA技术下调SIRT3表达后，观察H2S对AngⅡ诱导的心肌成纤维细胞增殖、羟脯氨酸的含量以及Ⅰ型胶原（ColⅠ）、Ⅲ型胶原（ColⅢ）和视神经萎缩蛋白1（OPA1）表达的影响。结果H2S可抑制AngⅡ诱导的心肌成纤维细胞增殖，降低羟脯氨酸含量，增强SIRT3蛋白表达（P＜0.05）。用干扰RNA技术下调SIRT3表达后，H2S对AngⅡ诱导的心肌成纤维细胞增殖抑制作用、羟脯氨酸含量降低作用均被抑制，且H2S降低ColⅠ、ColⅢ表达的作用和增强OPA1表达的作用也明显减弱。结论H2S依赖增加SIRT3表达来抑制AngⅡ诱导的心肌成纤维细胞增殖。 |
| ABSTRACT： | OBJECTIVE To investigate the inhibitory effect and the possible mechanism of hydrogen sulfide （H2S） on the proliferation of cardiac fibroblasts. METHODS The heart of neonatal SD rats was collected， and cardiac fibroblasts were separated with differential centrifugation. Using sodium hydrosulfide as the donor of H2S， the effects of H2S on the proliferation of cardiac fibroblasts induced by angiotensin Ⅱ （Ang Ⅱ）， hydroxyproline content and the expression of sirtuin 3 （SIRT3） protein were detected. After SIRT3 knockdown with siRNA technology， the effects of H2S on the proliferation of cardiac fibroblasts induced by Ang Ⅱ， hydroxyproline content， the expressions of collagen Ⅰ （Col Ⅰ）， collagen Ⅲ （Col Ⅲ ） and optic atrophy protein 1 （OPA1） were detected. RESULTS H2S could inhibit the proliferation of Ang Ⅱ-induced cardiac fibroblasts， reduce the content of hydroxyproline and increase the expression of SIRT3 （P＜0.05）. After down-regulating the expression of SIRT3 with siRNA technology， the inhibition of H2S on the proliferation of Ang Ⅱ-induced cardiac fibroblasts and the reduction of hydroxyproline content were both inhibited， and the effect of H2S on reducing the expression of Col Ⅰ and Col Ⅲ and enhancing the expression of OPA1 was also significantly weakened. CONCLUSIONS H2S inhibits the proliferation of Ang Ⅱ -induced cardiac fibroblasts through increasing the expression of SIRT3. |
| 期刊： | 2023年第34卷第04期 |
| 作者： | 刘露露；秦燕；孟国梁；顾锦华；张琳；包小燕 |
| AUTHORS： | LIU Lulu，QIN Yan，MENG Guoliang，GU Jinhua，ZHANG Lin，BAO Xiaoyan |
| 关键字： | 硫化氢；心肌成纤维细胞；血管紧张素Ⅱ；去乙酰化酶3 |
| KEYWORDS： | hydrogen sulfide； cardiac fibroblasts； angiotensin Ⅱ； sirtuin 3 |
| 阅读数： | 588 次 |
| 本月下载数： | 4 次 |
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