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姜黄素固体脂质纳米粒在大鼠体内的组织分布研究
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| 篇名： | 姜黄素固体脂质纳米粒在大鼠体内的组织分布研究 |
| TITLE： | Study on tissue distribution of curcumin solid lipid nanoparticles in rats |
| 摘要： | 目的 研究姜黄素固体脂质纳米粒（Cur-SLN）在大鼠体内的组织分布特点。方法采用微乳法制备Cur-SLN。将SD大鼠随机分为Cur原料药组和Cur-SLN组，每组45只。两组大鼠均单次静脉注射相应药物（以Cur计，注射剂量均为25mg/kg），分别于给药0.25、0.5、1、2、4、6、8、12、24h时，分离大鼠心、肺、肾和肝组织，采用高效液相色谱法测定Cur在不同组织中的含量，并分析其组织分布情况。结果Cur在心、肺、肾和肝组织中检测质量浓度的线性范围分别为0.06475～129.50、0.06475～64.75、0.06475～129.50、0.06475～129.50μg/mL（r均大于0.99），定量下限均为0.06475μg/mL，检测限均为0.01295μg/mL；日内、日间精密度以及准确度、提取回收率均符合生物样品定量分析的要求。与Cur原料药组比较，Cur-SLN组大鼠心、肾、肺（0.25～24h各时间点）和肝（0.25～1h、12～24h各时间点）组织样品中Cur的含量均显著升高（P＜0.05或P＜0.01）；而肝（2～8h各时间点）组织样品中Cur的含量均显著降低（P＜0.01）。结论将Cur制成固体脂质纳米粒后，增加了其在心、肾、肺组织的分布。 |
| ABSTRACT： | OBJECTIVE To study the tissue distribution characteristics of curcumin solid lipid nanoparticles （Cur-SLN） in rats. METHODS Cur-SLN was prepared with microemulsion. SD rats were randomly divided into Cur raw material group and Cur- SLN group， with 45 rats in each group. The rats of two groups were injected with the corresponding drugs （by Cur， 25 mg/kg） by single intravenous injection. The heart， lung， kidney and liver tisse were separated at 0.25， 0.5， 1， 2， 4， 6， 8， 12 and 24 h after administration. The contents of Cur in different tissues were determined by high-performance liquid chromatography method. Their tissue distribution was analyzed. RESULTS The linear range of detected mass concentration of Cur in heart， lung， kidney and liver tissues were 0.064 75-129.50， 0.064 75-64.75， 0.064 75-129.50， 0.064 75-129.50 μg/mL， respectively （all r＞0.99）. The lower limits of quantitation were all 0.064 75 μg/mL， and the limit of detection were all 0.012 95 μg/mL. The intra-day and inter-day precision， accuracy and extraction recovery were in line with the requirements of quantitative analysis. Compared with Cur raw material group， the contents of Cur in heart， kidney， lung （at each time point of 0.25-24 h） and liver tissue （at each time point of 0.25-1 h， 12-24 h） of samples were significantly increased in the Cur-SLN group （P＜0.05 or P＜0.01）， while the contents of Cur in liver tissue （at each time point of 2-8 h） were significantly decreased （P＜0.01）. CONCLUSIONS After Cur was prepared into solid lipid nanoparticles， its distribution in heart， kidney and lung tissues is increased. |
| 期刊： | 2023年第34卷第03期 |
| 作者： | 李旭；郝迪；王梓；李楠 |
| AUTHORS： | LI Xu，HAO Di，WANG Zi，LI Nan |
| 关键字： | 姜黄素；固体脂质纳米粒；组织分布；大鼠；高效液相色谱法 |
| KEYWORDS： | curcumin； solid lipid nanoparticles； tissue distribution； rat； high-performance liquid chromatography |
| 阅读数： | 158 次 |
| 本月下载数： | 3 次 |
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