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载美洲大蠊提取物蛛丝蛋白膜的制备、表征及体外释药性和细胞毒性考察
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| 篇名： | 载美洲大蠊提取物蛛丝蛋白膜的制备、表征及体外释药性和细胞毒性考察 |
| TITLE： | Preparation，characterization，in vitro drug release property and cytotoxicity of Periplaneta americana extract-loaded spider fibroin membrane |
| 摘要： | 目的 制备一种载美洲大蠊提取物的蛛丝蛋白膜，并对其进行表征及体外释药性和细胞毒性考察。方法以天然敬钊缨毛蛛蛛丝为原料，美洲大蠊提取物为模型药物，采用溶剂浇铸法制备载药蛛丝蛋白膜（后文称“载药膜”），同法制备不含美洲大蠊提取物的载药材料基质蛛丝蛋白膜（后文称“空白膜”）。经静态水接触角、傅里叶红外色谱、X-射线衍射以及扫描电镜等技术对膜剂进行表征，从不同角度分析膜剂结构；通过体外模拟药物在人工唾液中的释放特性评价药物的缓释性能；以MTT法验证载药膜的体外细胞毒性。结果制备得到的载药膜的静态水接触角均小于90°，且小于空白膜；载药膜在1500～1700cm－1存在美洲大蠊提取物多肽的特征吸收峰；同时X-射线衍射和扫描电镜也证实美洲大蠊提取物已成功负载到膜剂中。该膜剂可在人工唾液中释药时长逾200min。MTT实验结果显示，空白膜和载药膜作用后的细胞增殖率分别为84.6%、79.4%（均大于70%），无显著的潜在细胞毒性。结论以天然蛛丝所制备的载药膜在人工唾液中具有一定的缓释效果，有望作为一种具优异生物相容性的药物缓释载体应用于临床。 |
| ABSTRACT： | OBJECTIVE To prepare spider fibroin membrane loaded with Periplaneta americana extract， and investigate its characterization， in vitro drug release property and cytotoxicity. METHODS Using natural spider silk collected from Chilobrachys guangxiensis as raw material， P. americana extract as model drug， the drug-loaded spider fibroin membrane （hereinafter referred to as drug-loaded membrane） was prepared by solvent casting method. The material matrix spider fibroin membrane without P. americana extract （hereinafter referred to as blank membrane） was prepared with same method. The membrane structure was characterized by static water contact angle， Fourier infrared chromatography， X-ray diffraction and scanning electron microscopy from different angles； drug release characteristics in artificial saliva were simulated in vitro to evaluate the drug sustained-release performance. MTT assay was adopted to validate the cytotoxicity of drug-loaded membrane. RESULTS The drug-loaded membrane was prepared， and the static water contact angle was less than 90°， which was less than that of blank membrane. The drug-loaded membrane showed the characteristic absorption peak to polypeptide of P. americana extract at 1 500-1 700 cm－1. X-ray diffraction and scanning electron microscopy also proved that the drug was successfully loaded into the pellicle. The release time of the pellicle in artificial saliva was more than 200 min. The MTT test results showed that the cell proliferation rates of blank membrane and drug-loaded membrane were 84.6% and 79.4% （both greater than 70%）， respectively， without significant potential cytotoxicity. CONCLUSIONS Drug-loaded membrane prepared with natural spider silk has a certain sustained-release effect in artificial saliva， which can be further developed as a drug sustained-release carrier with excellent biological characteristics and biocompatibility. |
| 期刊： | 2023年第34卷第02期 |
| 作者： | 曾惠娜；卿晨；薛楠楠；杨自忠；巫秀美；李和伟；赵昱；李启艳 |
| AUTHORS： | ZENG Huina，QING Chen，XUE Nannan，YANG Zizhong，WU Xiumei，LI Hewei，ZHAO Yu，LI Qiyan |
| 关键字： | 美洲大蠊；口腔膜剂；天然蛛丝；敬钊缨毛蛛；蛛丝蛋白膜；药物缓释；含量测定；细胞毒性 |
| KEYWORDS： | Periplaneta americana； oral pellicles； natural spider silk； Chilobrachys guangxiensis； spider fibroin membrane； |
| 阅读数： | 150 次 |
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