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UPLC-Q-TOF/MS技术结合UNIFI平台快速鉴定鸡骨草化学成分
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| 篇名： | UPLC-Q-TOF/MS技术结合UNIFI平台快速鉴定鸡骨草化学成分 |
| TITLE： | Rapid identification of chemical constituents in Abrus cantoniensis Hance by UPLC-Q-TOF/MS combined with UNIFI platform |
| 摘要： | 目的 建立超高效液相色谱-四极杆-飞行时间质谱（UPLC-Q-TOF/MS）结合UNIFI平台的分析方法，对鸡骨草的化学成分进行快速鉴定。方法采用ACQUITYPRMHSST3FIT色谱柱，以0.1%甲酸水溶液-乙腈为流动相进行梯度洗脱，流速为0.3mL/min，进样量为2μL；采用电喷雾离子源，以全信息串联质谱技术在正、负离子模式下分别采集鸡骨草化学成分的质谱数据。建立鸡骨草化学成分数据库，基于UNIFI平台进行靶向和非靶向分析，结合化合物的精确分子质量、二级碎片离子信息及与对照品和文献数据等比对进一步鉴定化学成分。结果共鉴定了鸡骨草的46种化合物，包括黄酮类19种、三萜类8种、生物碱类3种、有机酸类5种、其他类11种；其中11种化合物在鸡骨草中首次报道，9种化合物经对照品比对确证。结论UPLC-Q-TOF/MS结合UNIFI平台的分析方法可快速鉴定鸡骨草的化学成分。鸡骨草中主要以黄酮类、三萜类成分为主。 |
| ABSTRACT： | OBJECTIVE To establish an analytical method based on ultra-performance liquid chromatography-quadrupole-time- of-flight mass spectrometry （UPLC-Q-TOF/MS） combined with UNIFI platform for rapid identification of the chemical constituents in Abrus cantoniensis Hance. METHODS The chromatographic separation was performed on ACQUITY PRM HSS T3 FIT column for gradient elution with the mobile phase consisted of 0.1% formic acid aqueous solution-acetonitrile. The flow rate was 0.3 mL/min， and injection volume was 2 μL. Electrospray ionization was used to collect the mass spectrometry data of the chemical constituents of A. cantoniensis Hance with full information tandem mass spectrometry technology in positive and negative ion modes. The chemical constituent database of A. cantoniensis Hance was established. Targeted and non-targeted analyses were conducted based on UNIFI platform， and chemical constituents were further identified in combination with accurate molecular mass， secondary fragment ion information and equivalence with reference substances and literature data， etc. RESULTS Totally 46 compounds of A. cantoniensis Hance were successfully identified， including 19 flavonoids， 8 triterpenoids， 3 alkaloids， 5 organic acids and 11 other components. Among them， 11 compounds were firstly found in A. cantoniensis Hance， and 9 compounds were confirmed by reference substance. CONCLUSIONS The analytical method based on UPLC-Q-TOF/MS combined with UNIFI platform can quickly identify the chemical constituents of A. cantoniensis Hance. Flavonoids and triterpenes are the main components in A. cantoniensis Hance. |
| 期刊： | 2022年第33卷第23期 |
| 作者： | 郭忠会，郑雪莹，梁洁，谭勇，覃春萍，郭家成，李耀华，韦志英，陈奎奎 |
| AUTHORS： | GUO Zhonghui， ZHENG Xueying，LIANG Jie，TAN Yong，QIN Chunping，GUO Jiacheng，LI Yaohua，WEI Zhiying，CHEN Kuikui |
| 关键字： | 鸡骨草；化学成分；鉴定；超高效液相色谱-四极杆-飞行时间质谱；UNIFI平台 |
| KEYWORDS： | Abrus cantoniensis Hance； chemical constituents； identification； UPLC-Q-TOF/MS； UNIFI platform |
| 阅读数： | 208 次 |
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