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金贝口服液抗小鼠甲型H1N1流感病毒及继发性肺炎链球菌感染作用研究
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| 篇名： | 金贝口服液抗小鼠甲型H1N1流感病毒及继发性肺炎链球菌感染作用研究 |
| TITLE： | Study on the effects of Jinbei oral liquid against influenza A H 1N1 virus and secondary Streptococcus pneumoniae infection of mice |
| 摘要： | 目的 研究金贝口服液体内抗小鼠甲型H1N1流感病毒及继发性肺炎链球菌感染的作用，为其临床应用提供参考。方法以磷酸奥司他韦胶囊（25.6mg/kg）为阳性对照进行实验。通过滴鼻含0.8个半数致死量（LD50）的H1N1病毒液复制小鼠甲型H1N1流感病毒感染模型，以体质量、肺指数、肺病毒载量、肺组织病理形态变化以及血清中肿瘤坏死因子α（TNF-α）、白细胞介素1β（IL-1β）、IL-6水平为指标，考察15.6、7.8、3.9mL/kg金贝口服液体内抗甲型H1N1流感病毒的作用。通过滴鼻含0.5个LD50的H1N1病毒液和含1×109个菌落形成单位的肺炎链球菌液复制继发性肺炎链球菌感染模型，以小鼠死亡情况、肺指数、鼻腔和肺部细菌载量以及血清中干扰素β（IFN-β）、IL-17、IL-23水平为指标，考察15.6mL/kg金贝口服液体内抗继发性肺炎链球菌感染的作用。结果15.6、7.8mL/kg金贝口服液给药6d后，均可显著升高甲型H1N1流感病毒感染小鼠异常降低的体质量，显著降低其异常升高的肺指数及血清中TNF-α、IL-1β、IL-6水平（P＜0.05），显著降低其肺部病毒载量（P＜0.05），减轻其肺组织病变程度。15.6mL/kg金贝口服液还可显著延长病毒-细菌共感染小鼠的存活时间（P＜0.05），降低其死亡率；显著升高其异常降低的体质量和血清中IL-17、IL-23水平（P＜0.05），显著降低其鼻腔和肺部细菌载量以及血清中异常升高的IFN-β水平（P＜0.05）。结论金贝口服液具有一定的抗小鼠甲型H1N1流感病毒感染作用，且能通过恢复17型免疫功能帮助机体抵抗继发性肺炎链球菌感染。 |
| ABSTRACT： | OBJECTIVE To study the effects of Jinbei oral liquid against influenza A H 1N1 virus and secondary Streptococcus pneumoniae infection of rats ，and to provide reference for its clinical application . METHODS Oseltamivir phosphate capsule （25.6 mg/kg）was used as a positive control . Influenza A H 1N1 virus infection model of mice was established by nasal drops of H 1N1 virus containing 0.8 median lethal dose （LD50）. The body mass ，lung index ，lung viral load ，pathological changes of lung tissue ， and serum levels of tumor necrosis factor α（TNF-α），interleukin-1β（IL-1β）and IL -6 were used as indexes to investigate the anti - H1N1 virus effect of 15.6，7.8 and 3.9 mL/kg Jinbei oral liquid in vivo . The model of secondary S. pneumoniae infection was established by nasal drops of H 1N1 virus solution containing 0.5 LD50 and S. pneumoniae solution containing 1×109 colony forming units. The death ，lung index ，nasal and lung bacterial load ，serum levels of interferon -β（IFN-β），IL-17 and IL -23 were used as indexes to investigate the effects of 15.6 mL/kg Jinbei oral liquid against secondary S. pneumoniae infection. RESULTS After 6 days of administration ，both 15.6 and 7.8 mL/kg Jinbei oral liquid significantly increased the abnormally reduced body weight of influenza A H 1N1 virus infected mice ，significantly reduced the abnormally increased lung index and serum levels of TNF -α，IL- 1β，IL-6（P＜0.05）；it also significantly reduced the viral load in the lung （P＜0.05）and alleviated the degree of lung tissue lesions. At the same time ，15.6 mL/kg Jinbei oral liquid significantly prolonged the survival time of mice co -infected 2020CXGC010505， with virus and bacteria （P＜0.05）and reduced the mortality rate；it also significantly increased the abnormally reduced body weight and serum levels of IL -17 and IL -23（P＜0.05）， while reduced the nasal and lung bacterial loads and the abnormally increased serum level of IFN -β （P＜0.05）. CONCLUSIONS Jinbei oral liquid has a certain anti -influenza A H 1N1 virus infection effect of rats and can help the body resist secondary S. pneumoniae infection by restoring the type 17 antibacterial immune function . |
| 期刊： | 2022年第33卷第21期 |
| 作者： | 赵方舒，张爱均，刘苗苗，田景振，侯林 |
| AUTHORS： | ZHAO Fangshu，ZHANG Aijun，LIU Miaomiao，TIAN Jingzhen，HOU Lin |
| 关键字： | 金贝口服液；甲型H1N1流感病毒；肺炎链球菌；继发性细菌感染 |
| KEYWORDS： | Jinbei oral liquid ；influenza A H 1N1；Streptococcus pneumoniae ；secondary bacterial infection |
| 阅读数： | 212 次 |
| 本月下载数： | 2 次 |
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