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桔贝合剂质量标准提升研究
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| 篇名： | 桔贝合剂质量标准提升研究 |
| TITLE： | Study on the improvement of quality standard for Jubei mixture |
| 摘要： | 目的 提升桔贝合剂的质量标准。方法采用三硝基苯酚与氢氰酸的化学反应鉴别苦杏仁水；采用薄层色谱（TLC）法定性鉴别桔贝合剂中桔梗、浙贝母、黄芩、枇杷叶、甘草；采用高效液相色谱（HPLC）法同时测定该制剂中苦杏仁苷、黄芩苷、汉黄芩苷、甘草酸铵4种成分的含量。结果三硝基苯酚试纸显砖红色。5味药材的TLC斑点清晰，阴性对照无干扰。苦杏仁苷、黄芩苷、汉黄芩苷和甘草酸铵的进样量线性范围分别为0.0668～1.3355μg（R2＝0.9994）、0.1898～3.7969μg（R2＝0.9999）、0.0626～1.2513μg（R2＝0.9999）、0.0418～0.8355μg（R2＝0.9998）；精密度、重复性、稳定性试验的RSD均小于2%；平均加样回收率分别为98.2%、99.5%、98.3%、99.6%，RSD分别为0.7%，1.6%、1.5%、1.1%（n＝6）。15批样品的含量存在差异，A公司样品的含量普遍低于B、C公司。结论所建质量标准可较好地反映桔贝合剂的内在质量。 |
| ABSTRACT： | OBJECTIVE To improve the quality standard for Jubei mixture . METHODS The chemical reaction of trinitrophenol and hydrocyanic acid was used to identify Armeniacae Amarum solution . Platycodon grandiflorus ，Fritillaria thunbergii，Scutellaria baicalensis ，the leaves of Eriobotrya japonica and Glycyrrhiza uralensis in Jubei mixture were identified by thin-layer chromatography （TLC）. High-performance liquid chromatography was used to simultaneously determine the contents of four active components ，such as amygdalin ，baicalin，wogonoside and monoammonium glycyrrhizinate in Jubei mixture . RESULTS Trinitrophenol test paper showed brick red . TLC spots of five medicinal materials were clear ，without interference from negative control . The linear ranges of amygdalin ，baicalin，wogonoside and monoammonium glycyrrhizinate were 0.066 8-1.335 5 μg（R2＝0.9994），0.189 8-3.796 9 μg（R2＝0.999 9），0.062 6-1.251 3 μg（R2＝0.999 9），0.041 8-0.835 5 μg（R2＝0.999 8）， respectively. RSDs of precision ，repeatability and stability tests were all lower than 2%. The average recoveries were 98.2%， 99.5%，98.3%，99.6%，and RSDs were 0.7%，1.6%，1.5%，1.1%（n＝6）. The contents of 15 batches of samples were different ， and the content of sample from company A was generally lower than those from companies B and C . CONCLUSIONS The standard established can reflect the inherent quality of Jubei mixture well . |
| 期刊： | 2022年第33卷第21期 |
| 作者： | 洪挺，周志强，肖小武，陈丹丹，杨毅生 |
| AUTHORS： | HONG Ting，ZHOU Zhiqiang，XIAO Xiaowu，CHEN Dandan，YANG Y isheng |
| 关键字： | 桔贝合剂；质量标准；化学反应；薄层色谱法；高效液相色谱法 |
| KEYWORDS： | Jubei mixture ； quality standard ； chemical reaction ； thin-layer chromatography ； high-performance liquid |
| 阅读数： | 169 次 |
| 本月下载数： | 3 次 |
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