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龙眼叶乙醇提取物在大鼠体内的代谢产物初步研究
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| 篇名： | 龙眼叶乙醇提取物在大鼠体内的代谢产物初步研究 |
| TITLE： | Preliminary study on metabolites derived from the ethanol extract from the leaves of Dimocarpus longan in rats in vivo |
| 摘要： | 目的 初步研究龙眼叶乙醇提取物在大鼠体内的代谢产物，为阐明龙眼叶降血糖的可能作用机制提供参考。方法以龙眼叶乙醇提取物、大鼠灌胃龙眼叶乙醇提取物33.8g/kg（以提取物计）后0～72h的粪便和0～48h的尿液，以及大鼠灌胃生理盐水（空白对照）后对应时段的粪便和尿液作为检测样品，采用超高效液相色谱-串联四极杆飞行时间质谱技术进行分析。收集化合物的精确相对分子量、分子式及碎片信息，通过与仪器自带数据库、谱库进行匹配，以及与对照品和相关文献进行比对，对化合物进行推测和鉴定。结果在大鼠粪便和尿液样品中共鉴定出了8个化合物，包括2个原型成分和6个代谢产物。从大鼠粪便样品中鉴定出了3个化合物，包括槲皮素、木犀草素2个原型成分以及木犀草素或山柰酚的1个代谢产物；从大鼠尿液样品中鉴定出了5个化合物（均为代谢产物），涉及槲皮素、木犀草素或山柰酚的代谢产物。代谢产物的类型主要为甲基化、葡萄糖醛酸化及氧化产物。结论龙眼叶乙醇提取物灌胃后在大鼠体内主要经甲基化、葡萄糖醛酸化等途径代谢，鉴定出的化合物多为槲皮素和木犀草素的代谢产物。 |
| ABSTRACT： | OBJECTIVE To study the metabolites derived from the ethanol extract from the leaves of Dimocarpus longan preliminarily in rats in vivo ，and to provide reference for elucidating the possible metabolic mechanism of the leaves of D. longan in lowering blood glucose . METHODS Ultra high performance liquid chromatography quadrupole time -of-flight mass spectrometry （UPLC-Q-TOF-MS/MS） was adopted by taking ethanol extract of D. longan leaves，the feces and urine of rats at 0-72 h and 0-48 h after intragastric administration of 33.8 g/kg ethanol extract of D. longan leaves（by extract ），the feces and urine of rats at the corresponding time after intragastric administration of normal saline （blank control ） as samples . The accurate relative molecular weight ，formula and fragment information of the compounds were collected ， and the compounds were speculated and i dentified by matching with the database and spectrum library of the instrument ，and comparing with the reference substance and relevant literature . RESULTS A total of eight compounds were identified in urine and feces of rats ，including 2 prototype components and 6 metabolites. Three compounds （including two prototype components as quercetin ，luteolin and one metabolite as luteolin or kaempferol） in feces of rats were identified ；five compounds （all metabolites ） in urine of rats were identified ，involving metabolites of quercetin ，luteolin or kaempferol . Metabolites mainly included the products of methylation ，glucuronidation and oxidation. CONCLUSIONS After intragastric administration ，the ethanol extract from the leaves of D. longan is mainly metabolized in rats through methylation ，glucuronidation and other pathways . The identified compounds are mostly metabolites of quercetin and luteolin . |
| 期刊： | 2022年第33卷第21期 |
| 作者： | 胡珏，黄光强，梁洁，柳贤福，曹玉嫔，陈奎奎，李耀华，安施佳，梁晶春 |
| AUTHORS： | HU Jue， HUANG Guangqiang，LIANG Jie，LIU Xianfu，CAO Yupin，CHEN Kuikui，LI Yaohua，AN Shijia，LIANG Jingchun |
| 关键字： | 龙眼叶；乙醇提取物；超高效液相色谱-串联四极杆飞行时间质谱；粪便；尿液；代谢产物 |
| KEYWORDS： | Leaves of Dimocarpus longan ；ethanol extract ；UPLC-Q-TOF-MS/MS；feces；urine；metabolites |
| 阅读数： | 96 次 |
| 本月下载数： | 1 次 |
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