益气活血中药组分对慢性脑缺血模型小鼠认知功能的影响及机制
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篇名: 益气活血中药组分对慢性脑缺血模型小鼠认知功能的影响及机制
TITLE: Effects of the components of traditional Chinese medicine for invigorating qi and activating blood circulation on cognitive function of chronic cerebral ischemia model mice and its mechanism
摘要: 目的 研究益气活血中药组分(人参总皂苷、银杏总酮酯、西红花总苷)对慢性脑缺血(CCI)模型小鼠认知功能的影响及相关机制。方法采用温控电流刺激C57BL/6J小鼠双侧颈总动脉制备CCI模型,随机将模型小鼠分为模型组、阿司匹林组(阳性对照,10mg/kg)和中药组(益气活血中药组分,33mg/kg),另设假手术组,每组16只。除模型组和假手术组小鼠灌胃水外,其余各组小鼠灌胃相应药液,每天1次,连续8周。检测各组小鼠跳台潜伏期、跳台错误次数,观察大脑皮层和海马组织中神经血管单元(NVU)超微形态结构,并检测脑组织中血管内皮生长因子(VEGF)、α7烟碱型乙酰胆碱受体(α7nAChRs)蛋白及mRNA的表达水平和VEGF、血管生成素1(Ang1)、碱性成纤维细胞生长因子(bFGF)的含量。结果与模型组比较,中药组小鼠跳台潜伏期显著延长(P<0.05),跳台错误次数显著减少(P<0.05);大脑皮层和海马组织中NVU超微形态结构异常明显改善,脑组织中VEGF、α7nAChRs蛋白及mRNA的表达水平以及VEGF、Ang1、bFGF的含量均显著升高(P<0.05)。结论益气活血中药组分能够改善CCI所致小鼠的认知功能障碍,其机制可能与保护脑内NVU,恢复受损NVU的超微形态结构,调控脑内与NVU及记忆功能密切相关蛋白的表达有关。
ABSTRACT: OBJECTIVE To investigate the effects of the compo nents of traditional Chinese medicine (TCM)(total ginsenoside,total ketone ester of Ginkgo biloba ,total glucoside of Crocus sativus )for invigorating qi and activating blood circulation on cognitive function of mice with chronic cerebral ischemia (CCI)and its related mechanisms . METHODS CCI model was established by stimulating bilateral common carotid arteries of C 57BL/6J mice with temperature controlled current . Model mice were randomly divided into model group ,aspirin group (positive control 10 mg/kg)and TCM group (the components of TCM for invigorating qi and activating blood circulation 33 mg/kg),sham operation group was set up additionally ,with 16 mice in each group. Except that the mice in model group and sham operation group were given water intragastrically ,the mice in the other groups were given corresponding medicine liquid intragastrically,once a day ,for 8 weeks. The latency of step down and the number of step -down errors of mice in each group were detected ;the ultrastructure of neurovascular units(NVU)in cerebral cortex and hippocampus was observed ;the protein and mRNA expressions of vascular endothelial growth factor(VEGF)and α7 nicotinic acetylcholine receptors (α7nAChRs) in cerebral tissue were detected ;the contents of VEGF,angiopoietin 1 (Ang1) and basic fibroblast growth factor(bFGF)were detected . RESULTS Compared with model group ,the latency of step -down was significantly prolonged (P< 0.05),and the number of step -down errors was significantly reduced (P<0.05)in TCM group . The ultrastructural abnormalities of NVU in cerebral cortex and hippocampus were significantly improved ,and the protein and mRNA expressions of VEGF and α7 nAChRs and the contents of VEGF ,Ang1 and bFGF in cerebral tissue were significantly increased (P<0.05). CONCLUSIONS The components of TCM for invigorating qi and activating blood circulation can improve CCI -induced cognitive dysfunction in mice,the mechanism of which may be associated with protecting NVU ,recovering ultrastructural abnormalities of injured NVU , and regulating the expression of proteins closely related to NVU and memory function .
期刊: 2022年第33卷第20期
作者: 韩富华,刘剑刚,孙林娟,李南南,官杰,詹敏,陈文洁
AUTHORS: HAN Fuhua,LIU Jiangang,SUN Linjuan,LI Nannan,GUAN Jie,ZHAN Min,CHEN Wenjie
关键字: 益气活血中药组分;人参总皂苷;银杏总酮酯;西红花总苷;慢性脑缺血;神经血管单元;血管性认知功能障碍;小鼠
KEYWORDS: the components of traditional Chinese medicine for invigorating qi and activating blood circulation ;total ginsenoside ;
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