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干姜和炮姜对脾胃虚寒型胃溃疡大鼠药效学指标及肠道菌群的影响
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| 篇名： | 干姜和炮姜对脾胃虚寒型胃溃疡大鼠药效学指标及肠道菌群的影响 |
| TITLE： | Effects of Zingiber officinale and processed Zingiber officinale on pharmacodynamic indexes and intestinal flora on gastric ulcer rats with spleen-stomach deficiency and cold type |
| 摘要： | 目的 探讨干姜和炮姜对脾胃虚寒型胃溃疡大鼠药效学指标及肠道菌群的影响。方法将SD大鼠随机分为正常组、模型组、阳性对照组（复方田七胃痛胶囊0.45g/kg）、干姜高剂量（15.0g/kg）和低剂量组（7.5g/kg）、炮姜高剂量（15.0g/kg）和低剂量组（7.5g/kg），每组10只。采用食醋（第1～10天）联合无水乙醇（第11天）灌胃法复制脾胃虚寒型胃溃疡大鼠模型。各给药组大鼠灌胃相应药液，正常组和模型组灌胃水（第5～10天）。灌胃无水乙醇1h后，于大鼠股动脉取血，检测各组大鼠血清胃动素（MTL）、胃泌素（GAS）、表皮生长因子（EGF）含量和凝血4项[活化部分凝血活酶时间（APTT）、凝血酶原时间（PT）、凝血酶时间（TT）、纤维蛋白原（FIB）]；计算各组大鼠胃组织的溃疡指数和溃疡抑制率；观察其病理形态学变化，并记录糜烂灶数量及面积；检测大鼠粪便样本中的肠道菌群。结果与模型组比较，各药物组血清MTL（炮姜低剂量组除外）、GAS（炮姜低剂量组除外）、EGF（干姜各剂量组除外）、FIB（干姜各剂量组除外）含量和溃疡抑制率（仅阳性对照组）均显著升高（P＜0.05），APTT（干姜各剂量组除外）、PT（仅炮姜高剂量组）、TT（干姜各剂量组除外）、溃疡指数（干姜各剂量组除外）、糜烂灶数量（干姜各剂量组除外）及面积（干姜各剂量组除外）均显著缩短或降低（P＜0.05），且炮姜对EGF、凝血4项（炮姜低剂量组的PT、TT、FIB除外）、溃疡指数（炮姜低剂量组除外）及抑制率的改善作用均优于相同剂量的干姜（P＜0.05）。与模型组比较，炮姜组大鼠肠道微生物的α多样性指数ACE、Shannon、Simpson均显著升高，其变形菌门相对丰度显著降低，而拟杆菌门相对丰度显著升高（P＜0.05），干姜组大鼠的粘液乳酸杆菌属相对丰度显著降低（P＜0.05）。结论干姜和炮姜均可通过提高GAS、MTL含量来改善脾胃虚寒症状，增强胃肠功能；炮姜能明显抑制脾胃虚寒型胃溃疡，这种作用与促进黏膜保护和修复、改善凝血功能、调整肠道菌群紊乱等有关。 |
| ABSTRACT： | OBJECTIVE To explore the influence of Zingiber officinale and processed Z. officinale on pharmacodynamic indexes and intestinal flora on gastric ulcer rats with spleen -stomach deficiency and cold type before and after processing with sand . METHODS The SD rats were randomly divided into normal group ，model group ，positive control group （Compound tianqi weitong capsule 0.45 g/kg），Z. officinale high-dose（15.0 g/kg）and low -dose（7.5 g/kg）groups，processed Z. officinale high- dose（15.0 g/kg）and low -dose（7.5 g/kg）groups，with 10 rats in each group . The rat model of gastric ulcer with spleen -stomach deficiency and cold type was established by intragastric administration of vinegar （day 1-10）and absolute ethanol （day 11）. Administration groups were given relevant liquid intragastrically ，and normal group and model group were given water intragastrically（day 5-10）. One hour after intragastric administration of absolute ethanol ，blood was taken from the femoral artery of rats ，the serum contents of motilin （MTL），gastrin（GAS），epidermal growth factor （EGF）as well as 4 items of blood coagulation [activated partial thromboplastin time （APTT），prothrombin time （PT），thrombin time （TT），fibrinogen（FIB）] were detect. The ulcer index and inhibition rate of ulcer in gastric tissue were calculated . The pathological changes of gastric tissue were observed，and the number and area of erosions were recorded . The diversity of gut microbiota in fecal samples of rats was detected . RESULTS Compared with model group ，the contents of MTL （except for processed Z. officinale low-dose group ），GAS（except for processed Z. officinale low-dose group ），EGF（except forofficinale groups） and FIB （except for Z. officinale groups），inhibitory rate of ulcer （only positive control group ）were all increased significantly （P＜0.05）. APTT（except for Z. officinale groups），PT（only processed Z. officinale high- dose group ），TT（except for Z. officinale groups），ulcer index （except for Z. officinale groups），the number （except for Z. officinale groups）and area of erosions （except for Z. officinale groups）were shortened and decreased significantly （P＜0.05）； improvement effects of processed Z. officinale were better than those of the same dose of Z. officinale on EGF ，4 items of blood coagulation（except for PT ，TT，FIB of processed Z. officinale low-dose group ），ulcer index （except for processed Z. officinale low-dose group ）and inhibitory rate of ulcer （P＜0.05）. Compared with model group ，α diversity indexes as ACE ，Shannon and Simpson of intestinal microorganisms in rats were increased significantly in processed Z. officinale group；the relative abundance of Proteobacteria was decreased significantly in processed Z. officinale group，while that of Bacteroidetes was increased significantly （P＜0.05）； the relative abundance of Limosilactobacillus was decreased significantly in Z. officinale group （P＜0.05）. CONCLUSIONS Z. officinale and processed Z. officinale can improve the symptoms of spleen -stomach deficiency and cold ，and enhance gastrointestinal function by increasing the content of GAS and MTL . Processed Z. officinale can significantly inhibit gastric ulcer of spleen -stomach deficiency and cold type ，which is related to the promotion of mucosal protection and repair ，improvement of coagulation functionand adjustment of gut microbiotadisorder . |
| 期刊： | 2022年第33卷第20期 |
| 作者： | 余凌英，李星，蔡平君，强梦琴，东宝花，蒋云秀，黄勤挽，陈志敏 |
| AUTHORS： | YU Lingying， LI Xing，CAI Pingjun，QIANG Mengqin，DONG Baohua，JIANG Yunxiu，HUANG Qinwan，CHEN Zhimin |
| 关键字： | 干姜；炮姜；脾胃虚寒；胃溃疡；胃泌素；胃动素；表皮生长因子；肠道菌群；炮制 |
| KEYWORDS： | Zingiber offcinale ；processed Z. officinale；spleen-stomach difficiency ；gastric ulcer ；gastrin；motilin；epidermal |
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