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龙胆环烯醚萜类成分对NAFLD细胞模型脂质积累及炎症的改善作用及机制
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| 篇名： | 龙胆环烯醚萜类成分对NAFLD细胞模型脂质积累及炎症的改善作用及机制 |
| TITLE： | Improvement effects and mechanism of gentian iridoids on lipid accumulation and inflammation in NAFLD cell model |
| 摘要： | 目的 研究龙胆环烯醚萜类成分（gentianiridoids，GI）对非酒精性脂肪性肝病（NAFLD）细胞模型脂质积累及炎症的改善作用及机制。方法以游离脂肪酸（0.5mmol/L）诱导的人肝癌HepG2细胞为NAFLD细胞模型。考察不同浓度（0.125、0.25、0.5、1、2、4mg/mL）GI对HepG2细胞活力的影响。将HepG2细胞分为对照组、模型组和GI低、中、高浓度组（0.25、0.5、1mg/mL），除对照组外，其余组均加入0.5mmol/L游离脂肪酸进行造模。培养24h后，考察HepG2细胞内脂滴形成的情况，检测细胞内三酰甘油（TG）含量，检测细胞上清液中天冬氨酸转氨酶（AST）、丙氨酸转氨酶（ALT）和炎症指标[白细胞介素1β（IL-1β）、IL-6、肿瘤坏死因子α（TNF-α）]的水平；检测细胞中固醇调节元件结合蛋白1c（SREBP-1c）、脂肪酸合成酶（FAS）mRNA和核因子κB（NF-κB）信号通路相关蛋白[NF-κB抑制蛋白α（IκBα）、NF-κB、磷酸化NF-κB（p-NF-κB）]的表达。结果HepG2细胞经不同浓度GI作用后，细胞活力均显著升高（P＜0.01）。与模型组比较，GI各浓度组细胞内红色脂滴明显减少，细胞核萎缩不明显、体积大小正常；细胞内TG含量（低浓度组除外），细胞上清液中AST、ALT、IL-1β、IL-6（低剂量组除外）、TNF-α水平，细胞中SREBP-1c、FASmRNA表达水平以及NF-κB蛋白磷酸化水平均显著降低（P＜0.05或P＜0.01），IκBα蛋白表达水平均显著升高（P＜0.01）。结论GI可减少NAFLD细胞模型的脂质积累，减轻炎症反应，其作用机制可能与抑制SREBP-1c/FAS及NF-κB信号通路有关。 |
| ABSTRACT： | OBJECTIVE To study the improvement effects and mechanism of gentian iridoids （GI）on lipid accumulation and inflammation in non -alcoholic fatty liver disease （NAFLD）model. METHODS Human hepatoma HepG 2 cells induced by free fatty acids（0.5 mmol/L）were selected as NAFLD cell models . The effects of GI at different concentrations （0.125，0.25，0.5，1，2，4 mg/mL）on HepG 2 cell viability were investigated . HepG2 cells were divided into control group ，model group and low ，medium and high concentration groups of GI （0.25，0.5 and 1 mg/mL）. Except for control group ，0.5 mmol/L free fatty acid was added in other groups for modeling .After 24 h of culture ，the formation of lipid droplets was observed in HepG 2 cells；the content of triglyceride（TG）was detected . The levels of aspartate aminotransferase （AST），alanine aminotransferase （ALT）and inflammation indexes [interleukin-1 β（IL-1 β），IL-6，tumor necrosis factor α（TNF-α）] in cell supernatant were determined . The mRNA expressions of sterol -regulatory element binding protein （SREBP-1c）and fatty acid synthase （FAS）as well as the expression of nuclear factor -κB（NF-κB）related proteins [NF-κB inhibitor protein α（IκBα），NF-κB，phosphorylated NF -κB（p-NF-κB）] were also detected . RESULTS After treated with different concentrations of GI ，the cell viability of HepG 2 cells was improved significantly（P＜0.01）. Compared with model group ，the number of red lipid droplets in the cells of each concentration group of GI was significantly reduced ，the nuclear atrophy was not obvious ，and the size was normal ；the content of TG （except for low concentration group ），and the levels of ALT ，AST，IL-1β，IL-6（except for low concentration group ）and TNF - α in cell supernatant were all decreased significantly ；mRNA expression of SREBP -1c and FAS and the phosphorylation level of NF -κB were also decreased significantly （P＜0.05 or P＜0.01）；the protein expression of I κBα was increased significantly （P＜0.01）. CONCLUSIONS GI can reduce the lipid accumulation and inflammatory response of NAFLD cell model induced by free fatty acids，and its mechanism may be related to the inhibition of SREBP-1c/FAS and NF -κB signaling pathway . |
| 期刊： | 2022年第33卷第19期 |
| 作者： | 王丽娟，翁丽丽，肖春萍，于前，寇柏鑫 |
| AUTHORS： | |
| 关键字： | 龙胆；环烯醚萜类成分；脂质积累；炎症反应；核因子κB信号通路；非酒精性脂肪性肝病 |
| KEYWORDS： | Gentiana scabra ；gentian iridoids ；lipid |
| 阅读数： | 146 次 |
| 本月下载数： | 1 次 |
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