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毛萼乙素通过Wnt/β-catenin通路抑制结肠癌细胞上皮间质转化
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| 篇名： | 毛萼乙素通过Wnt/β-catenin通路抑制结肠癌细胞上皮间质转化 |
| TITLE： | Inhibitory effects of eriocalyxin B on epithelial -mesenchymal transition in colon cancer cells through the Wnt/β-catenin pathway |
| 摘要： | 目的 研究毛萼乙素对结肠癌细胞上皮间质转化（EMT）的影响及机制。方法以结肠癌细胞HT29、HCT116为研究对象。采用划痕愈合实验和Transwell实验检测毛萼乙素对2种细胞迁移和侵袭能力的影响；采用Westernblot法检测毛萼乙素（1.0μmol/L）、毛萼乙素+Wnt/β-联蛋白（β-catenin）信号通路激动剂Licl（1.0μmol/L毛萼乙素+10mmol/LLicl）或毛萼乙素+Wnt/β-catenin信号通路抑制剂XAV939（1.0μmol/L毛萼乙素+10μmol/LXAV939）对2种细胞的EMT相关蛋白[E-钙黏蛋白（E-cadherin）、N-钙黏蛋白（N-cadherin）、锌指转录抑制因子Snail]和Wnt/β-catenin信号通路相关蛋白[β-catenin、原癌基因蛋白质c-myc、细胞周期蛋白D1（cyclinD1）、T细胞因子4（TCF4）]表达的影响。结果毛萼乙素可显著抑制2种细胞的侵袭和迁移（P＜0.01），并显著降低2种细胞中N-cadherin、Snail、β-catenin、c-myc、cyclinD1、TCF4表达水平，显著升高E-cadherin表达水平（P＜0.05或P＜0.01）。毛萼乙素与Licl联用后，2种细胞中β-catenin、c-myc、cyclinD1、TCF4及N-cadherin表达水平被逆转（P＜0.05或P＜0.01）；与XAV939联用后，2种细胞中β-catenin、N-cadherin的表达进一步下调（P＜0.05或P＜0.01），E-cadherin的表达进一步上调（P＜0.01），且细胞迁移率也显著降低（P＜0.01）。结论毛萼乙素可通过抑制Wnt/β-catenin通路激活抑制结肠癌细胞上皮间质转化。 |
| ABSTRACT： | OBJECTIVE To study the effects and m echanism of eriocalyxin B （EriB）on epithelial -mesenchymal transition （EMT）of colon cancer cells . METHODS The colon cancer cells HT 29 and HCT 116 as research objects . Scratch-healing assay and Transwell assay were used to detect the effects of EriB on migration and invasion of two kinds of cells . Western blot method was used to detect the effects of EriB （1.0 μmol/L），EriB+Wnt/β-catenin signaling pathway agonist Licl （1.0 μmol/L EriB+ 10 mmol/L Licl）or EriB+Wnt/ β-catenin signaling pathway inhibitor XAV 939（1.0 μmol/L EriB+ 10 μmol/L XAV 939）on the expressions of EMT related proteins [E-cadherin，N-cadherin，Snail] and Wnt/ β-catenin signaling pathway related proteins [β-catenin，c-myc， cyclin D 1，TCF4]. RESULTS EriB can significantly inhibit the invasion and migration of two kinds of cells （P＜0.01）. EriB significantly reduced the protein expressions of N -cadherin，Snail，β-catenin，c-myc，cyclin D 1 and TCF 4 while increases the protein expression of E -cadherin（P＜0.05 or P＜0.01）. After EriB combined with Licl ，the protein expressions of β-catenin，c- myc，cyclin D 1 and TCF 4 in the two kinds of cells were reverse d（P＜0.05 or P＜0.01）. After combined with XAV 939，the protein expressions of β-catenin and N -cadherin were further down -regulated（P＜0.05 or P＜0.01）；the protein expression of E -cadherin was further up -regulated（P＜0.01），while migration rate was decreased significantly （P＜0.01）. CONCLUSIONS EriB can inhibit the epithelial -mesenchymal transition of colon 19SXHZ0351） cancer cells by inhibiting the activation of Wnt/ β -catenin |
| 期刊： | 2022年第33卷第19期 |
| 作者： | 匡微，郑银彬，李雨奇，田平平，苏强，向小聪 |
| AUTHORS： | |
| 关键字： | 毛萼乙素；结肠癌；Wnt/β-catenin通路；上皮间质转化 |
| KEYWORDS： | eriocalyxin B ；colon cancer ；Wnt/β-catenin |
| 阅读数： | 154 次 |
| 本月下载数： | 3 次 |
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