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肉桂人参颗粒的含量测定、指纹图谱建立及差异标志物筛选
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| 篇名： | 肉桂人参颗粒的含量测定、指纹图谱建立及差异标志物筛选 |
| TITLE： | Content determination ，fingerprint establishment and differential markers screening of Rougui renshen granules |
| 摘要： | 目的 测定肉桂人参颗粒中4种主要成分的含量，建立指纹图谱，并筛选影响其质量的差异标志物。方法采用高效液相色谱（HPLC）法测定甘草酸铵、甘草苷、肉桂酸、桂皮醛的含量，并同法建立10批肉桂人参颗粒的HPLC指纹图谱，采用《中药色谱指纹图谱相似度评价系统（2012版）》进行相似度评价，确定共有峰；采用SPSS25.0、SIMCA14.1软件进行聚类分析、主成分分析和正交偏最小二乘法-判别分析，以变量重要性投影（VIP）值大于1为标准筛选影响样品质量的差异标志物。结果含量测定的方法学考察均符合相关要求，10批样品中甘草酸铵、甘草苷、肉桂酸、桂皮醛的含量分别为1.8084～2.7700、1.1372～1.4814、0.0765～0.0918、0.1309～0.4784mg/g。10批肉桂人参颗粒中共有16个共有峰，共指认了其中4个共有峰，分别为甘草苷（6号峰）、肉桂酸（10号峰）、桂皮醛（11号峰）、甘草酸铵（15号峰）；相似度均大于0.95。聚类分析结果显示，10批样品可聚为3类，其中S3为一类，S1～S2、S4～S5、S10为一类，S6～S9为一类；主成分分析结果显示，前3个主成分的累计方差贡献率为91.918％，分类结果与聚类分析一致；正交偏最小二法-判别分析结果显示，有4个共有峰的VIP值大于1，分别为11号峰（桂皮醛）、15号峰（甘草酸铵）、6号峰（甘草苷）和9号峰。结论所建指纹图谱及含量测定方法准确度高、重复性好，可用于肉桂人参颗粒的质量评价；甘草酸铵、桂皮醛、甘草苷等成分可能是影响肉桂人参颗粒质量的差异标志物。 |
| ABSTRACT： | OBJECTIVE To determine the conte nts of 4 main components in Rougui renshen granules ，and to establish the fingerprint and to screen differential markers affecting its quality. METHODS HPLC method was employed to determine the contents of ammonium glycyrrhizinate ，glycyrrhizin，cinnamic acid and cinnamaldehyde. HPLC fingerprints of 10 batches of Rougui renshen granules were established simultaneously. Similarity Evaluation System of Chromatographic Fingerprint of TCM （2012 edition）was used to evaluate the similarity and determine the common peak ；SPSS 25.0 and SIMCA 14.1 software were applied for cluster analysis （CA），principal component analysis （PCA）and partial least square-discriminant analysis （OPLS-DA）. The differential markers affecting sample quality were screened by using the variable importance in projection （VIP）value＞ 1 as standard. RESULTS The methodology of content determination met the relevant requirements. The contents of ammonium glycyrrhizinate，glycyrrhizin，cinnamic acid and cinnamaldehyde were 1.808 4-2.770 0，1.137 2-1.481 4，0.076 5-0.091 8 and 0.130 9-0.478 4 mg/g，respectively. A total of 16 common peaks were found in the fingerprints of 10 batches of Rougui renshen granules. Four chromatographic peaks were identified ，i.e. glycyrrhizin （peak 6），cinnamic acid （peak 10），cinnamaldehyde（peak 11）and ammonium glycyrrhizinate （peak 15）. The similarities of samples were ＞0.95. Results of CA showed that 10 batches of samples could be classified into three categories ：S3 was grouped into one category ；S1-S2，S4-S5 and S 10 were grouped into one category；S6-S9 were grouped into one category. The results of PCA showed that the cumulative contribution rate of the first three principal components was 91.918％，and the classification results were consistent with CA. The results of OPLS-DA showed that the four peaks with VIP value ＞1 were peak 11（cinnamaldehyde），peak 15（ammonium glycyrrhizinate ），peak 6（glycyrrhizin） and peak 9. CONCLUSIONS Established methods of content determination and fingerprint are accurate and reproducible ，and can be used for the quality evaluation of Rougui renshen granules. The components as ammonium glycyrrhizinate ，cinnamaldehyde， glycyrrhizin may be differential markers affecting the quality of Rougui renshen granules. |
| 期刊： | 2022年第33卷第16期 |
| 作者： | 高欢，胡北，李想，陈莹，许子华 |
| AUTHORS： | GAO Huan，HU Bei，LI Xiang，CHEN Ying，XU Zihua |
| 关键字： | 肉桂人参颗粒；高效液相色谱法；含量测定；指纹图谱；化学模式识别 |
| KEYWORDS： | Rougui renshen granules ；HPLC；content determination ；fingerprints；chemical pattern recognition |
| 阅读数： | 137 次 |
| 本月下载数： | 9 次 |
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