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炮制和煎煮方式对化痰祛湿活血方成分的影响
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| 篇名： | 炮制和煎煮方式对化痰祛湿活血方成分的影响 |
| TITLE： | Effects of processing and decoction methods on the components of Huatan qushi huoxue decoction |
| 摘要： | 目的 建立化痰祛湿活血方（HQHD）的指纹图谱，探究炮制和煎煮方式对其成分的影响。方法以丹酚酸B为参照，采用《中药色谱指纹图谱相似度评价系统（2012版）》建立10批HQHD原方混煎、原方单煎、炮制混煎、炮制单煎样品（“原方”指8味中药均为生品“；炮制”指泽泻、丹参、郁金、柴胡为炮制品，其余药味为生品“；单煎”指各药味单独煎煮后混合“；混煎”指各药味混合后煎煮）的高效液相色谱指纹图谱，并进行相似度评价、共有峰指认及归属、化学模式识别分析。结果10批HQHD原方混煎、原方单煎、炮制混煎、炮制单煎样品指纹图谱均各有37个共有峰，与对照指纹图谱的相似度均大于或接近于0.950；共指认出9个共有峰，分别为芦丁（12号峰）、橙皮苷（13号峰）、丹酚酸B（16号峰）、槲皮素（20号峰）、水飞蓟宾（22号峰）、木犀草素（23号峰）、橙黄决明素（29号峰）、23-乙酰泽泻醇C（34号峰）、柴胡皂苷b2（35号峰）；8味组方饮片对HQHD指纹图谱均有贡献。主成分分析结果显示，4种HQHD样品各自聚为一类。偏最小二乘法-判别分析的聚类结果与主成分分析一致。1、15、17、18、36号峰对应成分和丹酚酸B、木犀草素可能是影响原方混煎与炮制混煎样品质量的差异标志物，1、7、17～19号峰对应成分和丹酚酸B、橙皮苷可能是影响原方单煎与炮制单煎样品质量的差异标志物，1、17～19、36号峰对应成分和丹酚酸B、木犀草素可能是影响原方单煎与原方混煎样品质量的差异标志物，7、17～19、21号峰对应成分和橙皮苷、丹酚酸B、芦丁、木犀草素、橙黄决明素可能是影响炮制混煎与炮制单煎样品质量的差异标志物。结论建立的HQHD指纹图谱方法稳定、可靠；木犀草素、橙皮苷等是不同煎煮方式样品的质量差异成分，芦丁、橙皮苷、橙黄决明素等是炮制与否样品的质量差异成分。 |
| ABSTRACT： | OBJECTIVE To establish the fingerprint of Huatan qushi huoxue decoction （HQHD），and to explore the effects of processing and decoction methods on its components. METHODS Using salvianolic acid B as reference ，HPLC fingerprints of 10 batches of single and mixed decoction of crude drugs ，single and mixed decoction of processed products （original formula referred to 8 ingredients were crude drugs ；processed formula referred to processed products of Alisma orientale ，Salvia miltiorrhiza ， Curcumae Radix and Bupleuri Radix ，and crude drugs of other ingredients ；single decoction referred to the mixing of each ingredient after being decocted separately ；mixed decoction refers to decocting after mixing all ingredients ）were stablished by Similarity Evaluation System of Chromatographic Fingerprints of Traditional Chinese Medicine （2012 edition）. The similarity evaluation，common peak identification and attribution ，chemical pattern recognition analysis were also carried out. RESULTS There were 37 common peaks in each fingerprint of 10 batches of single and mixed decoction of crude drugs ，single and mixed decoction of processed ，the similarities with control fingerprint were higher than or close to 0.950. Nine common peaks were identified，i.e. rutin （peak 12），hesperidin（peak 13），salvianolic acid B （peak 16），quercetin（peak 20），silybin（peak 22）， luteolin（peak 23），autrantio-obtusin（peak 29），23-acetylalismol C （peak 34），saikosaponin b 2（peak 35）；decoction pieces of 8 ingredients all contributed to the fingerprints of HQHD. Principal component analysis （PCA）showed that the 4 kinds of HQHD samples were grouped into one category ，respectively. The clustering result of partial least squares-discriminant analysis was consistent with that of PCA. Corresponding components of peak 1，15，17，18 and 36，salvianolic acid B and luteolin m ay be the differential markers of the quality for mixed decoction samples of crude drugs and processed products ； corresponding components of peak 1，7，17-19，salvianolic acid B and hesperidin may be the differential markers of the quality for single decoction samples of crude drugs and processed products；corresponding components of peak 1，17-19，36， salvianolic acid B and luteolin may be the differential markers of the quality for single decoction and mixed decoction samples of crude drugs ；corresponding components of peak 7，17-19，21， hesperidin，salvianolic acid B ，rutin，luteolin and autrantio-obtusin may be the differential markers of the quality for single decoction and mixed decoction samples of processed products. CONCLUSIONS The established fingerprint of HQHD is stable and reliable. The quality differential components of different decoction samples are luteolin ，hesperidin，etc. The quality differential components of samples processed or not are rutin ，hesperidin，autrantio-obtusin，etc. |
| 期刊： | 2022年第33卷第16期 |
| 作者： | 刘鸣昊，黄亚森，张振凌，张丽慧，刘素彤，赵文霞 |
| AUTHORS： | LIU Minghao ，HUANG Yasen，ZHANG Zhenling ，ZHANG Lihui，LIU Sutong ，ZHAO Wenxia |
| 关键字： | 化痰祛湿活血方；炮制；煎煮方式；指纹图谱；主成分分析；偏最小二乘法-判别分析；差异成分 |
| KEYWORDS： | Huatan qushi huoxue decoction ；processing；decoction method ；fingerprint；principal component analysis ；partial |
| 阅读数： | 197 次 |
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