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加味塔布森-2提取工艺优化及其对破骨细胞分化的抑制作用
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| 篇名： | 加味塔布森-2提取工艺优化及其对破骨细胞分化的抑制作用 |
| TITLE： | Optimization of the extraction technology of modified Tabusen- 2 and its inhibitory effects on osteoclast differen- tiation |
| 摘要： | 目的 优化蒙药加味塔布森-2（MT-2）的提取工艺，并考察按最优工艺所制提取物对破骨细胞分化的抑制作用。方法采用网络药理学方法筛选MT-2工艺优化的指标性成分；以上述指标性成分的度值和含量计算综合评分，在单因素实验的基础上，使用Box-Behnken设计-响应面法优化MT-2的提取工艺并验证。以核因子κB受体激活蛋白配体（100ng/mL）诱导RAW264.7细胞以制备破骨细胞分化模型，考察按最优工艺所制MT-2提取物（18.6、37.2、74.4ng/mL）对破骨细胞分化的抑制作用。结果网络药理学筛选所得指标性成分包括绿原酸、松脂醇二葡萄糖苷、异绿原酸A、1，5-二咖啡酰奎宁酸、羟基红花黄色素A、人参皂苷Rg1、人参皂苷Rb1。MT-2最优提取工艺为乙醇体积分数60％，料液比1∶14（g/mL），提取时间94min，提取2次；3次验证实验所得平均综合评分为95.50，与预测值（95.75）的相对误差为－0.26％。与破骨分化模型细胞比较，经最优工艺所制MT-2提取物处理后的细胞多为单核圆形细胞，破骨细胞数量均显著减少（P＜0.05），且这种抑制作用有随药物质量浓度增加而增强的趋势。结论优化的MT-2提取工艺稳定、可行；所得提取物可抑制破骨细胞分化。 |
| ABSTRACT： | OBJECTIVE To optimize the extraction technology of modified Tabusen- 2（MT-2），and to investigate inhibitory effects of the extract obtained by the optimal technology on osteoclast differentiation. METHODS The index components of MT- 2 process optimization were selected by using network pharmacology. Based on single factor tests ，the extraction technology of MT- 2 was optimized by Box-Behnken design-response surface methodology according to the comprehensive score of contents of above index components ，and then validated. RAW 264.7 cells were induced by receptor activator of nuclear factor-κB ligand（100 ng/mL） to prepare osteoclast differentiation model. Inhibitory effects of MT- 2 extract（18.6，37.2，74.4 ng/mL）obtained by the optimal technology on osteoclast differentiation were investigated. RESULTS The index components screened by network pharmacology included chlorogenic acid ，terpineol diglucoside ，isochlorogenic acid A ，1，5-dicaffeoylquinic acid ，hydroxysafflower yellow A ， ginsenoside Rg 1 and ginsenoside Rb 1. The optimal extraction technology of MT- 2 was ethanol volume fraction of 60％ ，the solid-liquid ratio of 1 ∶ 14（g/mL），extraction time of 94 min and extraction times of twice. The average comprehensive score obtained by the three validation experiments was 95.50，and the relative error with the predicted value （95.75）was －0.26％. Compared with osteoclastic differentiation model cells ，the cells treated with MT- 2 extract prepared by the optimal technology were mostly mononuclear round cells ，and the number of osteoclasts decreased significantly （P＜0.05），its inhibitory effects tended to strengthen with the increase of drug concentration. CONCLUSIONS The optimal extraction technology of MT- 2 is stable and feasible. Obtained extract can inhibit osteoclast differentiation. |
| 期刊： | 2022年第33卷第16期 |
| 作者： | 郭姝，宋清翔，董睿，封千喜，薛培凤，董馨 |
| AUTHORS： | GUO Shu，SONG Qingxiang，DONG Rui，FENG Qianxi，XUE Peifeng，DONG Xin |
| 关键字： | 加味塔布森-2；蒙药；提取工艺；Box-Behnken设计-响应面法；破骨细胞分化；抑制作用 |
| KEYWORDS： | modified Tarbson- 2； Mongolian medicine ； extraction technology ； Box-Behnken design-response surface |
| 阅读数： | 156 次 |
| 本月下载数： | 3 次 |
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