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清解化攻方调节重症急性胰腺炎模型大鼠肠道菌群及对肠黏膜屏障的影响
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| 篇名： | 清解化攻方调节重症急性胰腺炎模型大鼠肠道菌群及对肠黏膜屏障的影响 |
| TITLE： | Study on the effects of Qingjie huagong decoction on the regulation of intestinal flora and intestinal mucosal barrier in severe acute pancreatitis model rats |
| 摘要： | 目的 研究清解化攻方调节重症急性胰腺炎（SAP）模型大鼠肠道菌群及对肠黏膜屏障的影响。方法采用雨蛙素联合脂多糖腹腔注射法复制SAP大鼠模型，观察各组大鼠生存状态，检测大鼠血清中血清淀粉酶、白细胞介素10（IL-10）、IL-18、IL-1β水平，观察大鼠胰腺及小肠组织的病理变化，检测大鼠小肠组织中闭合蛋白（Occludin）、闭锁连接蛋白1（ZO-1）、高迁移率族蛋白B1（HMGB1）的表达水平；利用16SrRNA高通量测序检测大鼠肠道菌群结构和相对丰度。结果经清解化攻方干预后，SAP模型大鼠腹部膨胀症状明显减轻，精神状态恢复较好；血清中血清淀粉酶、IL-18水平显著降低（P＜0.05），IL-10水平显著升高（P＜0.05）；胰腺组织坏死区域、炎症细胞浸润减少，肠上皮细胞结构紊乱程度有所缓解，肠黏膜上皮脱落减少；小肠组织中HMGB1蛋白表达水平显著降低（P＜0.05），Occludin、ZO-1蛋白表达水平均显著升高（P＜0.05）。16SrRNA高通量测序结果显示，清解化攻方可升高大鼠肠道拟杆菌门、乳酸杆菌属等益生菌群的相对丰度，减少厚壁菌门等有害菌群的定植。结论清解化攻方可通过上调小肠组织Occludin、ZO-1蛋白表达，下调HMGB1蛋白表达，改善肠黏膜屏障损伤，并可调节不同菌群的相对丰度从而达到保护肠道的作用。 |
| ABSTRACT： | OBJECTIVE To explore the the reg ulation of intestinal flora and effects of Qingjie huagong decoction on intestinal mucosal barrier in severe acute pancreatitis （SAP）mode rats . METHODS SAP rat model was induced by intraperitoneal injection of caerulein and lipopolysaccharide.The survival state of rats in each group were observed.The levels of serum amylase ，interleukin 10（IL-10），IL-18 and IL- 1β in serum were all detected. The pathological changes of pancreatic and small intestinal tissue were observed. The expressions of Occludin，ZO-1 and HMGB1 were detected in small intestinal tissue of rats. The structure and relative abundance of intestinal microflora in rats were detected by 16S rRNA high throughput sequencing. RESULTS After the intervention of Qingjie huagong decoction ，abdominal distension symptoms of SAP model rats were significantly relieved ，and their mental state recovered better ；the levels of serum amylase and IL- 18 in serum were decreased significantly （P＜0.05），while the level of IL- 10 was increased significantly （P＜0.05）. The necrotic area of pancreatic tissue and the infiltration of inflammatory cells were reduced ， the degree of intestinal epithelial cell structural disorder was alleviated ，and the shedding of intestinal mucosal epithelium was reduced.The protein expression of HMGB 1 in small intestinal tissue was decreased significantly （P＜0.05），and the protein expression of Occludin and ZO- 1 were increased significantly . Results of 16S rRNA high throughput sequencing showed that Qingjie huagong decoction could increased the relative abundance of probiotics such as Bacteroidea and Lactobacillus in rat intestine ，reduced the colonization of harmful bacteria such as Firmicutes. CONCLUSIONS Qingjie huagong decoction can improve the intestinal barrier by up-regulating the expression of Occludin and ZO- 1 in small intestinal tissue and down-regulating the protein expression of HMGB 1. It can also adjust the relative abundances of different flora to protect the intestinal tract. |
| 期刊： | 2022年第33卷第15期 |
| 作者： | 秦百君，唐曦平，杨昕，杨蕾，冯敏超，张驰，洪晓华，蓝艳梅，陈国忠 |
| AUTHORS： | QIN Baijun，TANG Xiping， YANG Xin，YANG Lei，FENG Minchao，ZHANG Chi，HONG Xiaohua，LAN Yanmei，CHEN Guozhong |
| 关键字： | 重症急性胰腺炎；清解化攻方；肠道菌群；肠黏膜屏障 |
| KEYWORDS： | severe acute pancreatitis ；Qingjie huagong decoction ；intestinal flora ；intestinal mucosal barrier |
| 阅读数： | 175 次 |
| 本月下载数： | 5 次 |
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