Box-Behnken响应面法结合多指标综合评分法优化龙眼叶提取工艺
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篇名: Box-Behnken响应面法结合多指标综合评分法优化龙眼叶提取工艺
TITLE: Optimization of the extraction technology of the leaves of Dimocarpus longan by Box-Behnken response surface methodology combined with multi-index comprehensive score
摘要: 目的 根据黄酮类和酚酸类成分优化龙眼叶的提取工艺。方法采用高效液相色谱法同时测定龙眼叶中没食子酸、原儿茶酸、没食子酸乙酯、槲皮素、木犀草素和山柰酚的含量。在单因素实验的基础上,以乙醇体积分数、料液比、提取时间为考察因素,上述6种成分含量的综合评分为考察指标,采用Box-Behnken响应面法优化龙眼叶的提取工艺。结果龙眼叶的最优提取工艺为乙醇体积分数100%,料液比1∶7(g/mL),提取时间90min,提取温度80℃。经3次实验验证,所得平均综合评分为97.54分(RSD=0.33%,n=3),与预测综合评分(99.05分)的相对误差为1.55%。结论Box-Behnken响应面法结合多指标综合评分法可用于龙眼叶的提取工艺优化,且所得最优提取工艺稳定、可行。
ABSTRACT: OBJECTI VE To optimize the extraction technology of the leaves of Dimocarpus longan according to flavonoids and phenolic acids. METHODS The contents of gallic acid ,protocatechuic acid ,ethyl gallate ,quercetin,luteolin and kaempferol in the leaves of D. longan were determined by HPLC. Based on single factor test ,with the ethanol volume fraction ,solid-liquid ratio and extraction time as factors ,using comprehensive scores of the contents of above six components as indexes ,the extraction technology of the leaves of D. longan was optimized by Box-Behnken response surface methodology. RESULTS The optimal extraction technology included ethanol volume fraction of 100%,solid-liquid ratio of l ∶ 7(g/mL),extraction time of 90 min, extraction temperature of 80 ℃. After 3 times of validation tests ,the average comprehensive score was 97.54(RSD=0.33%,n= 3),relative error of which with predicted score (99.05)was 1.55%. CONCLUSIONS Box-Behnken response surface methodology combined with multi-index comprehensive score can be used for the extraction technology of the leaves of D. longan ,and the optimized extraction technology is stable and feasible.
期刊: 2022年第33卷第14期
作者: 黄光强,郑飘雪,梁洁,陈奎奎,曹玉嫔,胡珏,安施佳,梁晶春,刘星晨,朱晓峰
AUTHORS: HUANG Guangqiang,ZHENG Piaoxue, LIANG Jie,CHEN Kuikui,CAO Yupin,HU Jue,AN Shijia,LIANG Jingchun,LIU Xingchen,ZHU Xiaofeng
关键字: 龙眼叶;Box-Behnken响应面法;综合评分;提取工艺;高效液相色谱法;含量测定
KEYWORDS: leaves of Dimocarpus longan ;Box-Behnken
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