环黄芪醇对四氯化碳致小鼠肝纤维化及糖酵解的影响
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篇名: 环黄芪醇对四氯化碳致小鼠肝纤维化及糖酵解的影响
TITLE: Effects of cycloastragenol on carbon tetrachloride-induced hepatic fibrosis and glycolysis in mice
摘要: 目的 研究环黄芪醇(CAG)对四氯化碳(CCl4)诱导的小鼠肝纤维化(HF)及糖酵解的影响。方法将雄性ICR小鼠随机分为空白组、模型组和CAG低、中、高剂量组(60、120、240mg/kg),每组6只。除空白组小鼠腹腔注射橄榄油外,其余各组小鼠均腹腔注射10%CCl4-橄榄油溶液(5mL/kg),每周3次,持续8周以复制HF模型。自造模第4周起,各药物组小鼠灌胃相应药液(10mL/kg),空白组和模型组小鼠灌胃0.5%羧甲基纤维素钠溶液(10mL/kg),每天1次,持续4周。实验过程中,称定小鼠的体质量;末次灌胃后,称定小鼠的肝脏质量并计算其肝脏指数,检测其肝损伤指标[天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)]、HF相关指标[苏木精-伊红(HE)染色评分、Masson和天狼星红染色胶原容积分数、Ⅰ型胶原蛋白(collagenⅠ)、α-平滑肌肌动蛋白(α-SMA)]和糖酵解相关指标[乳酸(LD)、己糖激酶(HK)、磷酸果糖激酶(PFK)、丙酮酸激酶(PK)]的变化情况。结果与模型组比较,各药物组小鼠胶原沉积和纤维化病变程度均有所减轻,体质量(CAG低剂量组除外)均有所升高(P<0.05),肝脏指数,血清ALT、AST水平,肝组织病理学HE染色评分、Masson和天狼星红染色胶原容积分数,collagenⅠ、α-SMA蛋白的表达水平,血清LD含量,以及血清、肝组织中HK、PFK、PK水平(CAG低剂量组肝组织除外)均显著降低(P<0.05)。结论CAG对CCl4诱导的小鼠HF有改善作用,同时降低了其体内糖酵解关键酶和产物水平。
ABSTRACT: OBJECTIVE To study the effects of cy cloastragenol (CAG) on carbon tetrachloride (CCl4)-induced hepatic fibrosis(HF)and glycolysis in mice. METHODS Male ICR mice were randomly divided into blank group ,model group ,CAG low-dose,medium-dose and high-dose groups (60,120,240 mg/kg),with 6 mice in each group. Except that blank group was given olive oil intraperitoneally ,the mice in other groups were intraperitoneally injected with 10%CCl4-olive oil solution (5 mL/kg) three times a week for 8 weeks to induce HF model. From the 4th week after modeling ,mice in each drug group were given corresponding drug solution intragastrically (10 mL/kg),and mice in blank group and model group were given 0.5% sodium carboxymethyl cellulose solution intragastrically (10 mL/kg),once a day for 4 weeks. During the experiment ,the body weight of mice were weighted ;after last gastrogavage ,the liver weight was weighted and liver indexes of mice were calculated. The changes of hepatic injury indexes [aspartate aminotransferase (AST), alanine aminotransferase (ALT)], related indexes of HF [hematoxylin-eosin (HE)staining score ,Masson and Picrosirius red staining collagen volume fraction ,collagen Ⅰ,α-smooth muscle actin (α-SMA)] and related indexes of glycolysis [lactic acid (LD),hexokinase(HK),phosphofructokinase(PFK), pyruvate kinase (PK)] were all detected. RESULTS Compared with model group ,the collagen deposition and fibrosis of mice in each drug group were reduced ,and the body weights of mice (except for CAG low-dose group )were increased to some extent (P<0.05). Liver indexes ,serum levels of ALT and AST ,HE staining score of liver histopathology ,Masson and Picrosirius red staining collagen volume fraction ,protein expression of collagen Ⅰ and α-SMA,serum content of LD ,the levels of HK ,PFK and PK in serum and hepatic tissues (except for hepatic tissue of CAG low-dose group )were all decreased significantly (P<0.05). CONCLUSIONS CAG can improve HF in mice induced by CCl 4,and reduce the levels of key enzymes and products of glycolysis.
期刊: 2022年第33卷第14期
作者: 吴红雁,顾亚琴,周红成,陈欣,张立虎
AUTHORS: WU Hongyan,GU Yaqin,ZHOU Hongcheng,CHEN Xin,ZHANG Lihu
关键字: 环黄芪醇;肝损伤;肝纤维化;糖酵解;小鼠
KEYWORDS: cycloastragenol;hepatic injury ;hepatic fibrosis ;glycolysis;mice
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