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条芩与枯芩的指纹图谱建立、抗炎活性及谱效关系研究
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| 篇名： | 条芩与枯芩的指纹图谱建立、抗炎活性及谱效关系研究 |
| TITLE： | Establishment of fingerprints ，anti-inflammatory activities and spectrum-effect relationship study of pith-nodecayed and pith-decayed Scutellaria baicalensis |
| 摘要： | 目的 建立条芩与枯芩的指纹图谱，考察两者的抗炎活性差异。方法采用高效液相色谱法建立5批条芩和5批枯芩的指纹图谱；采用SIMCA14.1软件，以变量重要性投影（VIP）值＞1为标准筛选影响条芩和枯芩质量的差异标志物；以其对脂多糖（LPS）诱导的小鼠单核巨噬细胞（RAW264.7细胞）上清液中一氧化氮（NO）、白细胞介素6（IL-6）、IL-1β的抑制率为指标，评价条芩和枯芩的抗炎活性并计算其抑制NO的半数有效浓度（EC50）；采用灰色关联分析谱效关系；以50μg/mL质量浓度下各组细胞的NO、IL-1β、IL-6抑制率为指标，采用SPSS26.0软件进行聚类分析。结果5批条芩和5批枯芩共有15个共有峰，相似度均大于0.990。共有8个峰的VIP值＞1，依次为峰14、12、15、6、10、13、11、4。在50μg/mL质量浓度下，枯芩提取物对NO、IL-6、IL-1β的抑制率及平均EC50分别为62.14％～71.13％、3.32％～18.38％、93.12％～95.47％、25.35μg/mL，条芩提取物分别为39.52％～50.19％、6.21％～22.55％、94.10％～96.44％、58.63μg/mL；枯芩提取物的平均NO抑制率显著高于条芩提取物，平均EC50、IL-6抑制率均显著低于条芩提取物（P＜0.05）；两者平均IL-1β抑制率比较差异无统计学意义（P＞0.05）。峰2～3、5～8、10～11与NO抑制率的关联度均大于0.8，峰2、5、8～9与IL-1β抑制率的关联度均大于0.9，15个共有峰与IL-6抑制率的关联度均小于0.8。10批样品可聚为2类，K1～K5为一类，T12～T16为一类。结论在50µg/mL质量浓度下，枯芩提取物对NO的抑制作用强于条芩提取物，对IL-6的抑制作用弱于条芩提取物，对IL-1β的抑制作用与条芩提取物相当；初步确定峰6、10～11对应成分为条芩与枯芩抗炎活性差异的主要化学成分。 |
| ABSTRACT： | OBJECTIVE To es tablish the fingerprints of pith-nodecayed and pith-decayed Scutellaria baicalensis ，and to investigate the difference of their anti-inflammatory activities. METHODS HPLC method was adopted to establish the fingerprints of 5 batches of pith-nodecayed S. baicalensis and 5 batches of pith-decayed S. baicalensis . SIMCA 14.1 software was adopted to screen the markers of weight difference between pith-decayed S. baicalensis and pith-nodecayed S. baicalensis ，using variable importance in projection （VIP）＞1 as standard. Using the inhibitory rates of NO ，IL-6 and IL- 1β in supernatant of lipopo- lysaccharide（LPS）-induced monocyte macrophages （RAW264.7 cells）as indexes ，the anti-inflammatory activities of pith-decayed and pith-nodecayed S. baicalensis were evaluated and median effective concentration （EC50）of NO were calculated. The gray correlation method was used for spectrum-effect relationship analysis ；SPSS 26.0 software was used for cluster analysis using the inhibitory rates of NO ，IL-6 and IL- 1β in cells at the concentration of 50 μg/mL. RESULTS There were 15 common peaks in 5 batches of pith-nodecayed S. baicalensis and 5 batches of pith-decayed S. baicalensis ，and the similarity of them was greater than 0.990. There were total of 8 peaks with VIP 1.0，they were peak 14，12，15，6，10，13，11 and 4. At jcqinge@163.com the concentration of 50 μg/mL，the inhibitory rates of pith- decayed S. baicalensis extract to NO ，IL-6，IL-1 β and the average EC 50 were 62.14％-71.13％，3.32％ -18.38％ and 93.12％-95.47％ and 25.35 μg/mL，respectively；those of pith-nodecayed S. baicalensis extract were 39.52％-50.19％， 6.21％-22.55％，94.10％-96.44％ and 58.63 μg/mL，respectively. Average NO inhibition rate of pith-decayed S. baicalensis extract was significantly higher than that of pith- nodecayed S. baicalensis extract；average EC 50 and average IL- 6 inhibitory rate of pith-decayed S. baicalensis extract were significantly lower than those of pith-nodecayed S. baicalensis extract（P＜0.05）. There was no statistical significance in average inhibitory rate of IL- 1β（P＞0.05）. The correlation degrees of peaks 2-3，5-8 and 10-11 with inhibitory rate of NO were all greater than 0.8. The correlation degrees of peaks 2，5，8-9 with inhibitory rate of IL- 1β were all greater than 0.9. The correlation degrees of 15 common peaks with inhibitory rate of IL- 6 were all less than 0.8. Ten batches of samples could be grouped into two categories ，K1-K5 were clustered into one category and T 12-T16 were clustered into one category. CONCLUSIONS At the concentration of 50 µg/mL，the inhibitory effect of pith-decayed S. baicalensis extract to NO is stronger than that of pith-nodecayed S. baicalensis extract，while the inhibitory effect on IL- 6 is weaker than that of pith-nodecayed S. baicalensis extract；they have similar inhibitory effect on IL- 1β. The corresponding components of peak 6，10-11 are preliminarily identified as main chemical components of the difference in anti-inflammatory activity between pith-decayed S. baicalensis and pith-nodecayed S. baicalensis . |
| 期刊： | 2022年第33卷第12期 |
| 作者： | 贾传青，郭兰萍，王晓，于宗渊，陈龙，董红敬 |
| AUTHORS： | JIA Chuanqing，GUO Lanping，WANG Xiao，YU Zongyuan，CHEN Long，DONG Hongjing |
| 关键字： | 黄芩；条芩提取物；枯芩提取物；指纹图谱；抗炎活性；谱效关系 |
| KEYWORDS： | Scutellaria baicalensis ； pith-nodecayed Scutellaria baicalensis extract； pith-decayed Scutellaria baicalensis |
| 阅读数： | 213 次 |
| 本月下载数： | 5 次 |
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