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蒙药三子散醇提物化学成分的HPLC-Q-Exactive-MS快速分析与鉴定
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| 篇名： | 蒙药三子散醇提物化学成分的HPLC-Q-Exactive-MS快速分析与鉴定 |
| TITLE： | Rapid analysis and identification of chemical components of ethanol extract from Mongolian medicine Sanzi san by HPLC-Q-Exactive-MS |
| 摘要： | 目的 系统研究三子散醇提物的化学组成，为阐明该方的药效物质基础提供参考。方法采用高效液相色谱-四极杆/静电场轨道阱高分辨质谱（HPLC-Q-Exactive-MS）技术进行分析。色谱条件：采用Shim-packGIST-HPC18色谱柱，以乙腈-0.1％甲酸溶液为流动相进行梯度洗脱，流速为1mL/min，柱温为40℃，进样量为10μL。质谱条件：采用电喷雾离子源，在正、负离子检测模式下检测。检测方式为FullMS/dd-MS2，FullMS分辨率为70000，dd-MS2分辨率为17500，扫描范围为m/z110～1200。通过与对照品、参考文献和自建数据库信息比对后对各离子峰进行鉴定。结果在蒙药三子散醇提物中共鉴定出化合物64个，包括9个黄酮类成分、13个环烯醚萜类成分、14个有机酸类成分、18个鞣质类成分、3个三萜类成分、3个氨基酸类成分和4个脂肪酸类成分。结论本研究发现蒙药三子散醇提物中主要含环烯醚萜类、鞣质类和黄酮类成分等，可能是三子散发挥药效的物质基础。 |
| ABSTRACT： | OBJECTIVE To systematic ally stu dy the chemic al components of ethanol extract from Sanzi san ，and to provide reference for clarifying the pharmacodynamic material basis of the formulation. METHODS HPLC-Q-Exactive-MS technology was adopted. The determination was performed on Shim-pack GIST-HP C 18 column with mobile phase consisted of acetonitrile- 0.1％ formic acid aqueous solution for gradient elution at the flow rate of 1 mL/min. The column temperature was 40 ℃，and sample size was 10 μL. Mass spectrometry conditions included the electrospray spray ion source was used for detection in positive and negative ion detection modes. Full MS/dd-MS 2 detection mode was adopted ，the resolution of Full MS was 70 000 and the resolution of dd-MS2 was 17 500. The scanning range was m/z 110-1 200. The ion peaks were identified by comparing with the information of control substances ，literature references and self-built database. RESULTS A total of 64 components were identified in the ethanol extract of Mongolian medicine Sanzi san ， including 9 flavonoids，13 iridoids，14 organic acids ，18 tannins，3 triterpenes，3 amino acids and 4 fatty acids. CONCLUSIONS The ethanol extract of Mongolian medicine Sanzi san mainly include iridoids ，tannins and flavonoids ，which might be the pharmacodynamic material basis of Sanzi san. |
| 期刊： | 2022年第33卷第11期 |
| 作者： | 李君，胡玉霞，张梦迪，王跃武，张谦，胡格吉胡，高峰，常福厚 |
| AUTHORS： | LI Jun，HU Yuxia，ZHANG Mengdi ，WANG Yuewu ，ZHANG Qian，HU Gejihu ，GAO Feng，CHANG Fuhou |
| 关键字： | 蒙药；三子散；高效液相色谱-四极杆/静电场轨道阱高分辨质谱；成分组成；结构鉴定 |
| KEYWORDS： | Mongolian medicine ；Sanzi san ；HPLC-Q- |
| 阅读数： | 248 次 |
| 本月下载数： | 10 次 |
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