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祛白片正丁醇部位对脱黑色素细胞模型的药效学研究
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| 篇名： | 祛白片正丁醇部位对脱黑色素细胞模型的药效学研究 |
| TITLE： | n-butanol part from Qubai tablet and study on its pharmacodynamics to de melanocyte model |
| 摘要： | 目的 研究祛白片正丁醇部位的化学成分，以及其对脱黑色素细胞模型的药效作用。方法制备祛白片正丁醇部位。采用超高效液相色谱-质谱联用（UPLC-MS）技术分析其化学成分。以小鼠B16黑色素瘤细胞为研究对象，建立脱黑色素细胞模型后分为模型组、阳性对照不同浓度组（8-甲氧补骨脂素10、50、100、150、200μmol/L）、溶媒组（以DMSO稀释而得）和祛白片正丁醇部位不同浓度组（10、50、100、150、200μg/mL），采用倒置显微镜观察细胞数量变化，并检测细胞增殖率、促黑色素生成率、酪氨酸酶活性促进率。结果在正、负离子模式下初步确定了祛白片正丁醇部位的53个化合物（正离子模式下29个，负离子模式下33个，重合9个），其中香豆素类化合物所占比例最大，其次是黄酮类化合物。祛白片正丁醇部位可使细胞数明显增加，且与作用时间和给药浓度呈正相关；其可显著升高细胞的增殖率、促黑色素生成率、酪氨酸酶活性促进率（P＜0.01）。结论香豆素类和黄酮类化合物可能是祛白片正丁醇部位发挥抗白癜风作用的物质基础；祛白片正丁醇部位的抗白癜风作用可能是通过促进酪氨酸酶活性实现的。 |
| ABSTRACT： | OBJECTIVE To stud y the chemical cons tituents of n-butanol part of Qubai tablet and its pharmacodynamic effect on the model of de melanocyte. METHODS The n-butanol part of Qubai tablet was prepared. The chemical constituents were analyzed by ultra-high performance liquid chromatography-mass spectrometry （UPLC-MS）. Taking mice B 16 melanoma cells as the research object ，the de melanocyte model was established and divided into model group ，positive control different concentration groups（8-methoxypsoralen 10，50，100，150，200 μmol/L），solvent group （diluted with DMSO ）and Qubai tablet n-butanol part different concentration groups （10，50，100，150，200 μmol/L）. The number of cells were observed by inverted microscope ，and the cell proliferation rate ，the rate of melanin production and promotion rate of tyrosinase activity were also detected. RESULTS In the positive and negative ion mode ，53 compounds in the n-butanol part of Qubai tablet were preliminarily determined （29 in the positive ion mode ，33 in the negative ion mode ，overlapping 9），of which coumarins accounted for the largest proportion ， followed by flavonoids. The n-butanol part of Qubai tablet could significantly increase the number of cells ，which was positively correlated with the action time and administration concentration. It could significantly increase the proliferation rate of cells ，the rate of melanin production and promotion rate of tyrosinase activity （P＜0.01）. CONCLUSIONS Coumarins and flavonoids may be the material basis for the anti-vitiligo effect of n-butanol part from Qubai tablet ；anti-vitiligo effect of n-butanol part of Qubai tablet may be realized by promoting tyrosinase activity. |
| 期刊： | 2022年第33卷第09期 |
| 作者： | 段松冷，顾红燕，年宏蕾，邢若，曾蔚欣 |
| AUTHORS： | DUAN Songleng ，GU Hongyan ，NIAN Honglei ，XING Ruo，ZENG Weixin |
| 关键字： | 祛白片；正丁醇部位；白癜风；超高效液相色谱-质谱联用；黑色素；酪氨酸酶 |
| KEYWORDS： | Qubai tablet ；n-butanol part ；vitiligo；UPLC- MS ；melanin；tyrosinase |
| 阅读数： | 167 次 |
| 本月下载数： | 5 次 |
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