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枸杞子中4种类胡萝卜素成分含量测定一测多评法的建立
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| 篇名： | 枸杞子中4种类胡萝卜素成分含量测定一测多评法的建立 |
| TITLE： | Establisment of quantitative analysis of multi-components by singer marker for content determination of 4 carotenoids in Lycium barbarum |
| 摘要： | 目的 建立同时测定枸杞子中玉米黄质、β-胡萝卜素、β-隐黄素棕榈酸酯、玉米黄素双棕榈酸酯含量的方法。方法枸杞子药材经正己烷-无水乙醇-丙酮-甲苯（10∶6∶7∶7，V/V/V/V）超声提取后，采用高效液相色谱法进行测定。以YMCC30为色谱柱，以甲醇-乙腈-水（81∶14∶5，V/V/V）为流动相A、二氯甲烷为流动相B进行梯度洗脱，柱温为20℃，流速为1.0mL/min，检测波长为450nm，进样量为20μL。以玉米黄质为参照物，计算β-胡萝卜素、β-隐黄素棕榈酸酯、玉米黄素双棕榈酸酯的相对校正因子，再以此计算上述成分的含量，并与外标法测定结果进行比较。结果玉米黄质、β-胡萝卜素、β-隐黄素棕榈酸酯、玉米黄素双棕榈酸酯检测质量浓度的线性范围分别为0.1194～2.9838、0.1217～1.5216、0.2859～5.7188、8.4605～211.5133μg/mL（R2均大于0.999）；精密度、重复性、稳定性（16h）试验的RSD均小于4％；平均加样回收率分别为103.34％、107.37％、105.64％、96.16％（RSD＜5％，n＝9）。β-胡萝卜素、β-隐黄素棕榈酸酯、玉米黄素双棕榈酸酯的平均相对校正因子分别为1.109、1.390、1.158；一测多评法与外标法所测上述成分含量的相对误差均在±1％之内。结论本研究所建立的高效液相色谱法联合一测多评法准确、稳定，可用于枸杞子中4种类胡萝卜素成分的含量测定及质量控制。 |
| ABSTRACT： | OBJECTIVE To establish a method for simultaneous determination of zeaxanthin ，β-carotene，β-cryptoxanthin palmitate and zeaxanthin dipalmitate in Lycium barbarum . METHODS L. barbarum was extracted with n-hexane-anhydrous ethanol-acetone-toluene（10∶6∶7∶7，V/V/V/V）by ultrasonic method. High performance liquid chromatography （HPLC）method was adopted. The determination was performed on YMC C 30 column with mobile phase A consisted of methanol-acetonitrile-water （81∶ 14 ∶ 5，V/V/V）and mobile phase B consisted of dichloromethane （gradient elution ）at the flow rate of 1.0 mL/min. The column temperature was set at 20 ℃. The detection wavelength was set at 450 nm，and sample size was 20 μL. Using zeaxanthin as control，the relative correction factors （RCFs）of β-carotene，β-cryptoxanthin palmitate and zeaxanthin dipalmitate were calculated ， and then the content of each component was calculated according to RCFs and compared with the results of external standard method（ESM）. RESULTS The linear range of zeaxanthin ，β-carotene，β-cryptoxanthin palmitate and zeaxanthin dipalmitate were 0.119 4-2.983 8，0.121 7-1.521 6，0.285 9-5.718 8，8.460 5-211.513 3 μg/mL（all R2＞0.999）. RSDs of precision ，repeatability and stability（16 h）tests were all less than 4％. The average recoveries were 103.34％，107.37％，105.64％，96.16％（RSD＜5％，n＝ 9）. The average RCFs of β-carotene，β-cryptoxanthin palmitate and zeaxanthin dipalmitate were 1.109，1.390，1.158. The relative errors of the content determination results by quantitative analysis of multi-components by singer marker （QAMS）and ESM were within ±1％. CONCLUSIONS The established HPLC-QAMS method is accurate and stable ，which can be used for the content determination and quality control of 4 carotenoids in L. barbarum . |
| 期刊： | 2022年第33卷第08期 |
| 作者： | 陈欢，马玲，苏晓娟，李艳婷，马霞，马学琴 |
| AUTHORS： | CHEN Huan，MA Ling，SU Xiaojuan ，LI Yanting ，MA Xia，MA Xueqin |
| 关键字： | 枸杞子；高效液相色谱法；一测多评法；玉米黄质；β-胡萝卜素；β-隐黄素棕榈酸酯；玉米黄素双棕榈酸酯 |
| KEYWORDS： | Lycium barbarum ；HPLC；QAMS；zeaxanthin；β-carotene；β-cryptoxanthin palmitate ；zeaxanthin dipalmitate |
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