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艾纳香挥发油萃取工艺优化及成分分析
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| 篇名： | 艾纳香挥发油萃取工艺优化及成分分析 |
| TITLE： | Optimization of extraction technology of volatile oil from Blumea balsamifera and analysis on its compositions |
| 摘要： | 目的 优化艾纳香挥发油的超临界二氧化碳（CO2）萃取工艺，并比较超临界CO2萃取法和水蒸气蒸馏法所得艾纳香挥发油的成分。方法采用超临界CO2萃取法提取艾纳香挥发油，以挥发油萃取率为指标，以萃取温度、萃取压力、萃取时间为因素，在单因素实验的基础上，采用正交实验优化超临界CO2萃取工艺；采用气相色谱-质谱法鉴定艾纳香挥发油的成分，采用峰面积归一化法计算各成分的相对含量；以水蒸气蒸馏法所得挥发油为参照，比较两种方法的挥发油萃取率、成分及含量。结果艾纳香挥发油最优超临界CO2萃取工艺为萃取压力30MPa、萃取温度50℃、萃取时间50min；经3次实验验证，得挥发油的平均萃取率为4.64％（RSD＝0.54％，n＝3）。从超临界CO2萃取法所得艾纳香挥发油中共鉴定出39种成分，如三十三烷、豆甾醇、角鲨烯等，从水蒸气蒸馏法所得挥发油中共鉴定出51种成分，如正三十烷、喇叭茶萜醇、葎草烯环氧化物等；两种提取方法所得艾纳香挥发油的萃取率分别为4.64％、0.99％，其中含花椒油素、L-龙脑、β-石竹烯等26种共有成分。除花椒油素（超临界CO2萃取法为34.829％，水蒸气蒸馏法为30.676％）、叶绿醇（超临界CO2萃取法为2.401％，水蒸气蒸馏法为1.273％）外，超临界CO2萃取法所得挥发油成分的相对含量均低于水蒸气蒸馏法。结论所得最优超临界CO2萃取工艺稳定、可行；虽然两种提取方法所得艾纳香挥发油成分与含量的差异较大，但主要均为醛酮类、烯类、醇类等成分。 |
| ABSTRACT： | OBJECTIVE To opti mize the supercritical CO 2 extraction technology of volatile oil from Blumea balsamifera ，and compare the components of the volatile oil from B. balsamifera obtained by supercritical CO 2 extraction and steam distillation. METHODS The volatile oil of B. balsamifera was extracted by supercritical CO 2 extraction. Using extraction rate of volatile oil as index，extraction temperature ，extraction pressure and extraction time as factors ，based on single-factor experiment ，orthogonal experiment was used to optimize the supercritical CO 2 extraction technology. Gas chromatography-mass spectrometry was used to identify the components of volatile oil from B. balsamifera . Peak area normalization was used to calculate the relative contents of each component. Taking the volatile oil obtained by steam distillation as a reference ，the extraction rates ，components and contents of volatile oil by the two methods were compared. RESULTS The optimal supercritical CO 2 extraction technology of volatile oil from B. balsamifera included extraction pressure of 30 MPa，extraction temperature of 50 ℃ and extracting for 50 min. After 3 times of validation tests ，average extraction rate of volatile oil was 4.64％（RSD＝0.54％，n＝3）. Thirty-nine components such as tritriacontane，stigmasterol，squalene were identified in the volatile oil of B. balsamifera obtained by supercritical CO 2 extraction； and 51 components such as triacontane ，ledol，humulene epoxide Ⅰ were identified by steam distillation. The extraction rate of volatile oil from B. balsamifera obtained by 2 methods were 4.64％ and 0.99％. A total of 26 common components were obtained ， such as xanthoxylin ，L-borneol，β-caryophyllene. Except for xanthoxyline （34.829％ by supercritical CO 2 extraction，30.676％ by steam distillation method ）and phytol （2.401％ by supercritical CO 2 extraction，1.273％ by steam distillation ），the relative contents of the components of volatile oil obtained by supercritical CO 2 extraction were lower than those of steam distillation. CONCLUSIONS The optimal supercritical CO 2 extraction technology is stable and feasible ；the components and contents of volatile oil obtained by two methods varies greatly ，and main compounds are aldehydes and ketones ，alkenes，alcohols and other components. |
| 期刊： | 2022年第33卷第08期 |
| 作者： | 赵超，徐文芬，孙庆文，孙开芬，陈滕 |
| AUTHORS： | ZHAO Chao，XU Wenfen ，SUN Qingwen ，SUN Kaifen ，CHEN Teng |
| 关键字： | 艾纳香；挥发油；超临界CO2萃取法；正交实验；水蒸气蒸馏法；气相色谱-质谱法 |
| KEYWORDS： | Blumea balsamifera ；volatile oil ；supercritical CO 2 extraction；orthogonal experiments ；steam distillation ；gas |
| 阅读数： | 229 次 |
| 本月下载数： | 2 次 |
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