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祛白片乙酸乙酯部位成分分析及其对小鼠脱黑色素细胞模型的药效学研究
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| 篇名： | 祛白片乙酸乙酯部位成分分析及其对小鼠脱黑色素细胞模型的药效学研究 |
| TITLE： | Component analysis of ethyl acetate fraction in Qubai tablet and its pharmacodynamic study on de melanocyte model of mice |
| 摘要： | 目的 研究祛白片乙酸乙酯部位成分及其对小鼠脱黑色素细胞模型的药效学作用，探寻祛白片抗白癜风作用的物质基础。方法采用萃取法获得祛白片乙酸乙酯部位，以超高效液相色谱-质谱联用（UPLC-MS）分析其成分。实验设模型对照组、溶媒对照组、8-甲氧补骨脂素（8-MOP）给药组（10、50、100、150、200μmol/L）和祛白片乙酸乙酯部位给药组（10、50、100、150、200μg/mL），通过建立小鼠黑色素瘤细胞的脱黑色素细胞模型，从细胞数量、细胞活力情况、黑色素形成、酪氨酸酶活性4个方面研究祛白片乙酸乙酯部位对脱黑色素细胞的影响。结果UPLC-MS成分分析初步确定了祛白片乙酸乙酯部位的64种化合物结构，其中14种化合物在正、负离子模式下均有检出，补骨脂类化合物占比最大，补骨脂色烯查尔酮在正、负离子模式下含量均最高。药效学研究结果显示，祛白片乙酸乙酯部位可使脱黑色素细胞数量增加，显著提高细胞增殖率、促黑色素生成率及黑色素形成过程中酪氨酸酶活性促进率（P＜0.01）。结论补骨脂类化合物可能是祛白片乙酸乙酯部位发挥抗白癜风作用的物质基础；祛白片乙酸乙酯部位良好的抗白癜风作用可能与提高酪氨酸酶活性有关。 |
| ABSTRACT： | OBJECTIVE To study the components of ethyl acetate fraction in Qubai tablet ，and its pharmacodynamics on de melanocyte model ，and explore the material basis for anti-vitiligo effect of Qubai tablet. METHODS The ethyl acetate fraction of Qubai tablets was obtained by extraction ，and its components were analyzed by ultra performance liquid chromatography-mass spectrometry（UPLC-MS）. Model control group ，vehicle control group and 8-methoxypsoralen（8-MOP）administration groups （10，50，100，150，200 μmol/L），ethyl acetate fraction administration groups of Qubai tablet （10，50，100，150，200 μg/mL） were set up in the experiment. By establishing the de melanocyte model ，the effects of ethyl acetate fraction of Qubai tablet on de melanocyte were studied from four aspects ：cell number ，cell viability ，melanin formation and tyrosinase activity. RESULTS UPLC-MS component analysis preliminarily determined the structure of 64 compounds in the ethyl acetate fraction of Qubai tablet ， of which 14 compounds were detected in positive and negative ion mode ；psoralen compounds accounted for the largest proportion ， and the content of psoralen chromone chalcone was the highest in positive and negative ion mode. The results of pharmacodynamic study showed that the ethyl acetate fraction of Qubai tablet could increase the number of de melanocytes ，and significantly improve the cell proliferation rate ，the rate of promoting melanin formation and the rate of promoting tyrosinase activity in the process of melanin formation （P＜0.01）. CONCLUSIONS Psoralen compounds may be the material basis for the anti-vitiligo effect of ethyl acetate fraction of Qubai tablet ；good anti-vitiligo effect of ethyl acetate fraction of Qubai tablet may be related to the promotion of tyrosinase activity. |
| 期刊： | 2022年第33卷第07期 |
| 作者： | 段松冷，顾红燕，年宏蕾，邢若，姜德春 |
| AUTHORS： | DUAN Songleng ，GU Hongyan ，NIAN Honglei ，XING Ruo，JIANG Dechun |
| 关键字： | 祛白片；乙酸乙酯部位；成分分析；脱黑色素细胞模型 |
| KEYWORDS： | Qubai tablet ；ethyl acetate fraction ；component |
| 阅读数： | 216 次 |
| 本月下载数： | 4 次 |
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