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益心巴迪然吉布亚颗粒中5种有效成分的含量测定方法及其指纹图谱研究
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| 篇名： | 益心巴迪然吉布亚颗粒中5种有效成分的含量测定方法及其指纹图谱研究 |
| TITLE： | Study on the content determination method of five active components and fingerprints of Yixin badiran jibuya granules |
| 摘要： | 目的 建立益心巴迪然吉布亚颗粒中5种有效成分的含量测定方法及其指纹图谱。方法采用高效液相色谱法测定10批（编号S1～S10）益心巴迪然吉布亚颗粒中木犀草素-7-O-β-D-葡萄糖醛酸苷（LG）、芹菜素-7-O-葡萄糖醛酸苷（APG）、迷迭香酸（RA）、香叶木素-7-O-β-D-葡萄糖醛酸苷（DG）、田蓟苷（TL）的含量。采用《中药色谱指纹图谱相似度评价系统（2004A版）》绘制10批益心巴迪然吉布亚颗粒的指纹图谱，并进行相似度评价和聚类分析。结果10批益心巴迪然吉布亚颗粒中LG、APG、RA、DG、TL的含量分别为0.2795～0.4499、0.0824～0.1353、0.1848～0.4721、0.1490～0.3326、0.3112～0.6233mg/g；其指纹图谱中共有13个共有峰，指认出2号峰为LG、6号峰为APG、7号峰为RA、8号峰为DG、11号峰为TL，相似度为0.598～0.990。进一步聚类分析发现，S6～S10可聚为一类，S1～S5可聚为一类。结论本研究建立的含量测定方法和指纹图谱，可用于益心巴迪然吉布亚颗粒的质量控制。 |
| ABSTRACT： | OBJECTIVE To establish the method for t he content determination of 5 active components in Yixin badiran jibuya granules and their fingerprints. METHODS High performance liquid chromatography method was adopted to determine the contents of luteolin- 7-O-β-D-glucuronide（LG），apigenin-7-O-glucuronide（APG），rosmarinic acid （RA），diosmetin-7-O-β-D-glucuronide （DG）and tilianin （TL）in 10 batches（No. S 1-S10）of Yixin badiran jibuya granules. The fingerprints of 10 batches of Yixin badiran jibuya granules were drawn by Similarity Evaluation System of Chromatographic Fingerprint of Traditional Chinese Medicine（2004 A edition ），and similarity evaluation and cluster analysis were also performed. RESULTS The contents of LG ， APG，RA，DG and TL in 10 batches of Yixin badiran jibuya granules were 0.279 5-0.449 9，0.082 4-0.135 3，0.184 8-0.472 1， 0.149 0-0.332 6，0.311 2-0.623 3 mg/g，respectively. A total of 13 common peaks were demarcated in the fingerprints and were identified as LG （peak 2）APG（peak 6），RA（peak 7），DG（peak 8），TL（peak 11）. The similarity ranged from 0.598 to 0.990. The results of cluster analysis showed that S 6-S10 were clustered into one category and S 1-S5 were clustered into one category. CONCLUSIONS Established method for content determination and fingerprints can be used for the quality control for Yixin badiran jibuya granules. |
| 期刊： | 2022年第33卷第07期 |
| 作者： | 李红红，帕依曼·亥米提，王新春，何承辉 |
| AUTHORS： | LI Honghong，Payman·Hermity，WANG Xinchun，HE Chenghui |
| 关键字： | 益心巴迪然吉布亚颗粒；高效液相色谱法；含量测定；指纹图谱；质量控制 |
| KEYWORDS： | Yixin badiran jibuya granules ；high performance liquid chromatography ；content determination ；fingerprint； |
| 阅读数： | 388 次 |
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