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海藻羊栖菜化学成分全谱分析及其体外抗神经炎症活性研究
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| 篇名： | 海藻羊栖菜化学成分全谱分析及其体外抗神经炎症活性研究 |
| TITLE： | Full spectrum analysis of chemical constituents of Sargassum fusiforme and its in vitro anti-neuroinflammatory activity |
| 摘要： | 目的 研究羊栖菜的化学成分组成及体外抗神经炎症活性，为其开发利用以及药效物质研究提供参考。方法基于超高效液相飞行时间质谱联用技术（UHPLC-QTOF-MS/MS）和气相色谱-质谱联用技术（GC-MS/MS）对羊栖菜中化学成分进行全谱分析。利用脂多糖（1μg/mL）建立小鼠小胶质细胞BV2的炎症模型，以帕罗西汀（5μg/mL）为阳性对照药物，通过CCK-8法检测20、40、60、80、100μg/mL羊栖菜提取物作用24h后对细胞活性及形态的影响，通过酶联免疫吸附法检测40、60、80μg/mL羊栖菜提取物作用24h后对细胞上清液中肿瘤坏死因子α（TNF-α）、白细胞介素6（IL-6）含量的影响。结果经UHPLC-QTOF-MS/MS分析共鉴定出103种非挥发性成分，经GC-MS/MS分析共鉴定出60种挥发性成分。40、60、80μg/mL羊栖菜提取物可显著降低因脂多糖诱导而异常升高的BV2细胞活化程度和细胞上清液中TNF-α、IL-6的含量（P＜0.05或P＜0.01）。结论本研究建立了海藻羊栖菜的化学成分全谱，并证实了羊栖菜具有一定的体外抗神经炎症活性。 |
| ABSTRACT： | OBJECTIVE To study the composition of chemical constituents of Sargassum fusiforme and its in vitro anti- neuroinflammatory activity ，and to provide reference for its development and utilization and the study of pharmacodynamic substances. METHODS UHPLC-QTOF-MS/MS analysis method and GC-MS/MS method were used to analyze the chemical constituents of S. fusiforme . The lipopolysaccharide （1 μg/mL）was adopted to establish the inflammatory model of neuromicroglia BV2. Using paroxetine （5 μg/mL）as positive control ，CCK-8 assay was used to detect the effects of the extracts of S. fusiforme （20，40，60，80，100 μg/mL）on the activity and morphology of neuromicroglia BV 2. The effects of the extracts of S. fusiforme （40，60，80 μg/mL）on the contents of tumor necrosis factor α（TNF-α）and interleukin- 6（IL-6）in cell supernatant were detected by ELISA. RESULTS A total of 103 non-volatile constituents were identified by UHPLC-QTOF-MS/MS ，and 60 volatile constituents were obtained by GC-MS/MS. The extracts of S. fusiforme （40，60，80 μ g/mL） could significantly reduce the abnormally increased activation of neuromicroglia BV 2 and the contents of TNF-α and IL-6 due to lipopolysaccharide （P＜0.05 or P＜0.01）. CONCLUSIONS The study establish the full spectrum of chemical constituents of S. fusiforme ，and it is confirmed that fusiforme has certain in vitro anti-neuroinflammatory activity. |
| 期刊： | 2022年第33卷第07期 |
| 作者： | 彭红，黄品哲，宋永贵，徐焕华，周明月，朱根华，杨明，艾志福，苏丹 |
| AUTHORS： | PENG Hong， HUANG Pinzhe，SONG Yonggui，XU Huanhua，ZHOU Mingyue，ZHU Genhua，YANG Ming，AI Zhifu，SU Dan |
| 关键字： | 羊栖菜；全谱分析；挥发性成分；非挥发性成分；抗神经炎症活性 |
| KEYWORDS： | Sargassum fusiforme ；full spectrum analysis ； |
| 阅读数： | 193 次 |
| 本月下载数： | 11 次 |
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