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马齿苋炭炮制工艺优化及其对痔疮模型大鼠症状的改善作用
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| 篇名： | 马齿苋炭炮制工艺优化及其对痔疮模型大鼠症状的改善作用 |
| TITLE： | Optimization of processing technology of Portulaca oleracea charcoal and its improvement effect on the symptom of hemorrhoid model rats |
| 摘要： | 目的 优化马齿苋炭的炮制工艺，并探讨其对痔疮模型大鼠的改善作用。方法采用Box-Behnken设计-响应面法，以鞣质含量、水溶性浸出物含量以及外观性状的综合评分为指标，考察炒制温度、投药量、炒制时间对马齿苋炭炮制工艺的影响，优选出最优工艺参数，并进行验证。以最优炮制工艺制得的马齿苋炭（0.8g/mL）外敷痔疮模型大鼠，每天给药1次，连续11d；末次给药后，对大鼠肛周病理情况进行评分，并检测其血清中肿瘤坏死因子α（TNF-α）、白细胞介素6（IL-6）、IL-1β的水平。结果马齿苋炭最优炮制工艺为炒制温度200℃、投药量150g、炒制时间14min。最优炮制工艺的验证实验结果显示，马齿苋炭的综合评分为92.57，与预测值（96.56）的相对误差为－4.13％。经最优炮制工艺制得的马齿苋炭以0.8g/mL的糊状外敷，可明显促进痔疮模型大鼠创面愈合，显著减少渗出液量，显著降低血清中TNF-α、IL-6、IL-β水平。结论优化后的马齿苋炭炮制工艺具有可行性；经最优炮制工艺制得的马齿苋炭对痔疮模型大鼠症状具有良好的改善作用。 |
| ABSTRACT： | OBJ ECTIVE To optimize th e p rocessing technology of Portulaca oleracea charcoal，and to investigate its improvement effect on the symptom of hemorrhoid model rats. METHODS The effects of roasting temperature ，dosage and roasting time on the processing technology of P. oleracea charcoal were investigated with Box-Behnken response surface methodology using comprehensive score of tannin content ，water-soluble extract content and appearance properties as the index. The optimal process parameters are selected and verified. The hemorrhoid model rats were treated with P. oleracea charcoal（0.8 g/mL）prepared by the optimal processing technology ，once a day ，for 11 days. After last medication ，the perianal pathological score of hemorrhoid model rats were performed ；serum levels of tumor necrosis factor α（TNF-α），interleukin 6（IL-6）and IL- 1β were detected. RESULTS The optimal processing technology of P. oleracea charcoal included roasting temperature of 200 ℃， dosage of 150 g and roasting time of 14 min. Results of validation test showed that the comprehensive score of P. oleracea charcoal was 92.57，and relative error of it with predicted value （96.59）was －4.13％. External use of P. oleracea charcoal 0.8 g/mL prepared by the optimal processing technology could significantly promote the wound healing of hemorrhoid model rats ，reduced the amount of exudate ，and decreased the levels of TNF-α，IL-6 and IL-β in serum. CONCLUSIONS The optimized processing technology of P. oleracea charcoal is feasible. P. oleracea charcoal prepared by the optimized processing technology has good curative effect on the symptom of hemorrhoid model rats. |
| 期刊： | 2022年第33卷第05期 |
| 作者： | 王炳然，陈晶，李昕曈，蒋常鹏，张淼，马启风，高红梅 |
| AUTHORS： | WANG Bingran，CHEN Jing，LI Xintong，JIANG Changpeng，ZHANG Miao，MA Qifeng，GAO Hongmei |
| 关键字： | 马齿苋炭；炮制工艺；工艺优化；痔疮；大鼠 |
| KEYWORDS： | Portulaca oleracea charcoal；processing technology ；technology optimization ；hemorrhoid；rat |
| 阅读数： | 261 次 |
| 本月下载数： | 4 次 |
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