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人参皂苷Rb1通过调节TGF-β1/Smad3信号通路抑制肾小管上皮细胞-间质转化
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| 篇名： | 人参皂苷Rb1通过调节TGF-β1/Smad3信号通路抑制肾小管上皮细胞-间质转化 |
| TITLE： | Inhibition of ginsenoside Rb 1 on the epithelial-mesenchymal transition of renal tubular epithelial cells by regulating TGF-β1/Smad3 signaling pathway |
| 摘要： | 目的 研究人参皂苷Rb1（G-Rb1）对肾小管上皮细胞-间质转化（EMT）的影响及可能机制。方法采用10ng/mL生长因子β1（TGF-β1）诱导人肾小管上皮细胞HK-2发生EMT。观察10、20、30μmol/LG-Rb1作用48h后HK-2细胞的形态学变化；测定1.0、2.5、5.0、10、20、30μmol/LG-Rb1作用24h后人胚胎肾细胞HEK293中报告基因载体SBE转录活性，并测定上述浓度G-Rb1作用24h后对HK-2细胞相对活力的影响。检测10、20、30μmol/LG-Rb1作用24h后HK-2细胞中α-平滑肌肌动蛋白（α-SMA）、Ⅰ型胶原蛋白（COL-Ⅰ）、纤连蛋白（FN）mRNA的表达；检测10、20、30μmol/LG-Rb1作用24h后HK-2细胞中α-SMA、Smad家族成员3（Smad3）、磷酸化Smad3（p-Smad3）、COL-Ⅰ、FN和E-钙黏蛋白（E-cadherin）的表达。结果10～30μmol/LG-Rb1能够抑制TGF-β1诱导的HK-2细胞发生EMT，显著抑制因TGF-β1刺激导致的SBE转录活性升高（P＜0.05），并且对HK-2细胞的相对活力无明显影响（P＞0.05）。在TGF-β1诱导下，细胞中α-SMA、COL-Ⅰ、FN蛋白及其mRNA和Smad3、p-Smad3蛋白的表达均显著上调（P＜0.05），E-cadherin蛋白的表达显著下调（P＜0.05）；而G-Rb1则可有效地逆转上述蛋白或mRNA的表达。结论G-Rb1可在一定程度上保护肾小管上皮细胞免受TGF-β1诱导引起的EMT，这可能与其抑制了TGF-β1/Smad3信号通路的激活有关。 |
| ABSTRACT： | OBJECTIVE To study the effects of ginsenoside Rb 1（G-Rb1）on epithelial-mesenchymal transition （EMT）of renal tubular epithelial cells and its potential mechanism. METHODS The growth factor β1（TGF-β1）10 ng/mL was used to induce EMT of human renal tubular epithelial cells HK- 2. The morphological changes of HK- 2 cells were observed after treated with 10， 20，30 μmol/L G-Rb1 for 48 h. The transcriptional activities of biovector SBE in human embryonic kidney cell HEK 293 were determined after 24 h treatment with 1.0，2.5，5.0，10，20，30 μmol/L G-Rb1. Effects of above concentration of G-Rb 1 on the viability of HK- 2 cells were determined after 24 h of treatment. mRNA expressions of α-smooth muscle actin （α-SMA），collagen Ⅰ （COL-Ⅰ）and fibronectin （FN）in HK- 2 cells were detected after treated with 10，20，30 μmol/L G-Rb1 for 24 h. The expressions of α-SMA，Smad3，p-Smad3，COL-Ⅰ，FN and E-cadherin were detected after treated with 10，20，30 μmol/L G-Rb1 for 24 h. RESULTS G-Rb1 of 10-30 μmol/L significantly inhibited TGF-β1-induced EMT in HK- 2 cells and the increase of transcriptional activities of biovector SBE induced by TGF-β1（P＜0.05），but had no effects on relative activities of HK- 2 cells（P＞0.05）. The protein and mRNA expressions of α-SMA，COL-Ⅰ and FN ， the protein expressions of Smad 3 and p-Smad 3 were significantly up-regulated induced by TGF-β1（P＜0.05），while the protein expression of E-cadherin was significantly down- regulated（P＜0.05）；G-Rb1 could effectively reverse aboveprotein or mRNA expressions. CONCLUSIONS G-Rb1 can protect renal tubular epithelial cells from EMT induced byxiezhishen TGF-β1 to a certain extent ，which may be related to inhibiting the activation of TGF-β1/Smad3 signaling pathway. |
| 期刊： | 2022年第33卷第05期 |
| 作者： | 刘志文，徐江雁，张振强，张效威，高改，王萌萌，王辉，李真珍，谢治深 |
| AUTHORS： | LIU Zhiwen，XU Jiangyan， ZHANG Zhenqiang，ZHANG Xiaowei，GAO Gai，WANG Mengmeng，WANG Hui，LI Zhenzhen，XIE Zhishen |
| 关键字： | 人参皂苷Rb1；TGF-β1/Smad3信号通路；转化生长因子β1；肾小管上皮细胞；上皮细胞-间质转化 |
| KEYWORDS： | ginsenoside Rb 1；TGF-β1/Smad3 signaling pathway ；transforming growth factor β1；renal tubular epithelial cells ； |
| 阅读数： | 240 次 |
| 本月下载数： | 8 次 |
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