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特木仁-5味散的指纹图谱建立及4种成分的含量测定
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| 篇名： | 特木仁-5味散的指纹图谱建立及4种成分的含量测定 |
| TITLE： | Establishment of the fingerprint of Temurin- 5 powder and content determination of 4 components |
| 摘要： | 目的 建立特木仁-5味散的指纹图谱并进行化学模式识别分析，同时测定其中4种成分的含量。方法采用高效液相色谱法结合《中药色谱指纹图谱相似度评价系统（2012版）》建立10批特木仁-5味散（编号S1～S10）的指纹图谱并进行相似度评价，与混合对照品比对指认共有峰。采用SPSS26.0软件对共有峰进行系统聚类分析、主成分分析。采用高效液相色谱法测定10批样品中没食子酸、栀子苷、绿原酸、鞣花酸的含量。结果从10批特木仁-5味散的指纹图谱中共标定15个共有峰，相似度为0.997～0.999；指认峰1为没食子酸、峰3为栀子苷、峰5为绿原酸、峰12为鞣花酸。10批样品中，S4、S9聚为一类，S6～S8聚为一类，其余批次样品聚为一类；前3个主成分的累计方差贡献率为89.245％。没食子酸、栀子苷、绿原酸、鞣花酸检测质量浓度的线性范围分别为5.55～177.5、15.98～511.5、2.56～82.0、13.48～431.5μg/mL；精密度、稳定性（24h）、重复性试验的RSD均小于2％（n＝6或n＝7）；平均加样回收率分别为101.56％、102.21％、98.60％、96.62％，RSD分别为1.90％、1.61％、1.58％、1.73％（n＝6）。10批特木仁-5味散中，上述成分的平均含量分别为5.03～5.64、10.38～12.16、1.40～1.69、6.47～7.11mg/g。结论所建指纹图谱稳定、可行，含量测定方法符合相关规定，结合化学模式识别分析可用于特木仁-5味散的质量控制。 |
| ABSTRACT： | OBJECTIVE To establish the fing erprint of Temurin- 5 powder，conduct chemical pattern recognition analysis ，and determine the contents of 4 components simultaneously. METHODS The fingerprints of 10 batches of Temurin- 5 powder were established and similarity evaluation was performed by using high performance liquid chromatography （HPLC）combined with the Similarity Evaluation System of Chromatographic Fingerprints of Traditional Chinese Medicine （2012 edition）；common peaks were identified by comparing with mixed substance control. The common peaks were analyzed by systematic cluster analysis and principal component analysis with SPSS 26.0 software. The HPLC method was used to determine the contents of gallic acid ， geniposide，chlorogenic acid and ellagic acid in 10 batches of samples. RESULTS A total of 15 common peaks were identified from the fingerprints of 10 batches of Temurin-5 powder，and the similarity was 0.997-0.999. It was identified that peak 1 was gallic acid ，peak 3 was geniposide ，peak 5 was chlorogenic acid and peak 12 was ellagic acid. Among the 10 batches of samples ， S4 and S 9 were grouped into one category ，S6-S8 were grouped into one category ，and the other batches of samples were grouped into one category. The accumulative variance contribution rate of first three principal components was 89.245％. The linear ranges of gallic acid ，geniposide，chlorogenic acid and ellagic acid were 5.55-177.5，15.98-511.5，2.56-82.0 and 13.48-431.5 μg/mL， respectively. RSDs of precision ，stability（24 h）and repeatability tests were all less than 2％（n＝6 or n＝7）. The average recoveries were 101.56％，102.21％，98.60％ and 96.62％，respectively，RSDs were 1.90％，1.61％，1.58％ and 1.73％（n＝6）. Average contents of above components were 5.03-5.64，10.38-12.16，1.40-1.69，6.47-7.11 mg/g，respectively. CONCLUSIONS The established fingerprint is stable and feasible ，and the content determination method meets the relevant regulations. Combined with chemical pattern recognition analysis ，it can be used for the quality control of Temurin- 5 powder. |
| 期刊： | 2022年第33卷第04期 |
| 作者： | 孙灿灿，周婧，苏比依努尔·吐尔洪，马桂芝 |
| AUTHORS： | SUN Cancan ，ZHOU Jing，Subiyinuer·Tuerhong，MA Guizhi |
| 关键字： | 特木仁-5味散；指纹图谱；系统聚类分析；主成分分析；含量测定 |
| KEYWORDS： | Temurin-5 powders；fingerprint；systematic cluster analysis ；principal component analysis ；content determination |
| 阅读数： | 218 次 |
| 本月下载数： | 6 次 |
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