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不同产地诃子中11种成分的含量测定及其化学计量学综合评价
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| 篇名： | 不同产地诃子中11种成分的含量测定及其化学计量学综合评价 |
| TITLE： | Content determination of 11 components in Terminalia chebula from different origins and comprehensive evaluation of their chemometrics |
| 摘要： | 目的 建立不同产地诃子中11种成分的含量测定方法，为其质量评价及优质种源的筛选提供参考。方法以不同产地的16批诃子为检测样品，建立高效液相色谱串联三重四极杆质谱法测定诃子中牡荆素、没食子酸、没食子酸甲酯、没食子酸乙酯、鞣花酸、柯里拉京、莽草酸、阿魏酸、木犀草素、槲皮素、芦丁11种成分的含量。采用Shim-packGIST-HPC18色谱柱，以0.1％甲酸溶液-甲醇为流动相进行梯度洗脱，流速为0.25mL/min，进样量为3μL，柱温为35℃；采用电喷雾电离源，在正、负离子电离模式下进行检测，扫描方式为多反应监测模式，雾化气流量为3L/min，加热气流量为10L/min，接口温度为300℃，脱溶剂温度为526℃，加热块温度为400℃。运用灰色关联分析（GRA）和逼近理想解排序（TOPSIS）法对不同产地诃子进行比较分析和综合评价。结果含量测定方法学考察结果符合相关要求。16批诃子样品中上述11种成分的含量分别为7.27～106.38、5370.24～31010.43、21.42～1097.50、4.26～111.09、17940.42～38490.18、6247.26～40182.18、12125.94～209519.96、2.71～9.04、0.24～44.12、1.49～9.17、25.35～126.51μg/g。GRA和TOPSIS分析结果均显示，样品H12（产地为云南）、H11（产地为广西）、H5（产地为湖南）、H14（产地为广东）、H13（产地为四川）、H8（产地为广东）、H1（产地为云南）的综合质量相对较好。结论所建方法快速灵敏、准确可靠，可用于诃子的内在质量综合评价和优质种源筛选。 |
| ABSTRACT： | OBJECTIVE To establi sh the method for the con tent determination of 11 components in Terminalia chebula from different origins ，and to provide reference for their quality evaluation and superior provenance screening. METHODS Taking 16 batches of T. chebula from different origins as test samples ，high performance liquid chromatography tandem triple quadrupole mass spectrometry was established to determine the contents of 11 components，such as vitexin ，gallic acid ，methyl gallate ，ethyl gallate，ellagic acid ，corilagin，shikimic acid ，ferulic acid ，luteolin，quercetin and rutin. The determination was performed on Shim-pack GIST-HP C 18 column with mobile phase consisted of 0.1％ formic acid solution-methanol at the flow rate of 0.25 mL/ min（gradient elution ）. The sample size was 3 μL，and the column temperature was 35 ℃. Electrospray ionization source was used in positive and negative ion mode ，with multiple reaction monitoring. The atomized gas flow rate was 3 L/min，the heating gas flow rate was 10 L/min，the interface temperature was 300 ℃，the desolvent temperature was 526 ℃，and the heating block temperature was 400 ℃ . Grey correlation analysis （GRA）and technique for order preference by similarity to ideal solution （TOPSIS）methods were used to compare ，analyze and comprehensively evaluate T. chebula from different origins. RESULTS The results of content determination methodology met the relevant requirements. The contents of 11 components in 16 batches of T. chebula were 7.27-106.38，5 370.24-31 010.43，21.42-1 097.50，4.26-111.09，17 940.42-38 490.18，6 247.26-40 182.18，12 125.94- 209 519.96，2.71-9.04，0.24-44.12，1.49-9.17 and 25.35-126.51 μg/g，respectively. The results of GRA and TOPSIS analysis showed that the comprehensive qualities of sample H 12（from Yunnan ），H11（from Guangxi ），H5（from Hunan ），H14（from Guangdong），H13（from Sichuan ），H8（from Guangdong ），H1（from Yunnan ）were better. CONCLUSIONS The established method is fast ，sensitive and reliable ，and can be suitable for comprehensive evaluation of the internal quality and superior provenance screening of T. chebula . |
| 期刊： | 2022年第33卷第03期 |
| 作者： | 久欣，李君，张慧文，白云霞，刘宏，王焕芸 |
| AUTHORS： | JIU Xin，LI Jun，ZHANG Huiwen ，BAI Yunxia ，LIU Hong，WANG Huanyun |
| 关键字： | 诃子；高效液相色谱串联三重四极杆质谱；含量测定；灰色关联分析；逼近理想解排序法 |
| KEYWORDS： | Terminalia chebula ；high performance liquid |
| 阅读数： | 311 次 |
| 本月下载数： | 5 次 |
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