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芹菜素丝素蛋白纳米粒的制备及其安全性、抗肿瘤活性研究
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| 篇名： | 芹菜素丝素蛋白纳米粒的制备及其安全性、抗肿瘤活性研究 |
| TITLE： | Study on preparation ，safety and anti-tumor activity of apigenin silk fibroin nanoparticles |
| 摘要： | 目的 制备芹菜素丝素蛋白（API@SF）纳米粒，并评价其安全性和抗肿瘤活性。方法采用纳米沉淀法制备API@SF纳米粒，并对其形态、粒径、Zeta电位、载药量、体外释放等进行表征；采用溶血实验和HE染色法评价该纳米粒的安全性；采用MTT法评价该纳米粒对小鼠乳腺癌4T1细胞的抑制作用。结果本研究所制得的API@SF纳米粒呈类球形，粒径分布均匀，平均粒径为406.61nm，多分散性指数为0.154，Zeta电位为－18.4mV，平均载药量为5.20％。体外释放结果显示，该纳米粒在pH5.0的释放介质中释放速率相对较快，在pH7.4的释放介质中释放速率相对较慢。溶血实验和HE染色结果显示，该纳米粒具有良好的生物相容性。MTT实验结果显示，API@SF纳米粒对4T1细胞的抑制作用显著高于API原料药（P＜0.05），其作用机制可能与提高细胞中活性氧水平有关。结论本研究成功制备了API@SF纳米粒，该纳米粒具有良好的安全性和抗肿瘤活性。 |
| ABSTRACT： | OBJECTIVE To prepare apigenin silk fibroin（API@SF）nanoparticles and to evaluate their safety and anti-tumor activity. METHODS API@SF nanoparticles were prepared by nanoprecipitation method ，and their morphology ，particle size ，Zeta potential，drug loading amount and in vitro release were characterized. The safety of nanoparticles was evaluated by hemolysis test and HE staining. MTT assay was adopted to evaluate inhibitory effects of API@SF nanoparticles on breast cancer 4T1 cells in mice. RESULTS The prepared API@SF nanoparticles were spherical with uniform distribution. The average particle size was 406.61 nm， the polydispersity index was 0.154，the Zeta potential was －18.4 mV，and the average drug-loading amount was 5.20％. The in vitro release results showed that the release rate of the nanoparticles was relatively fast in the release medium of pH 5.0 and relatively slow in the release medium of pH 7.4. Results of hemolysis test and HE staining showed that the nanoparticles had good biocompatibility. Results of MTT assay showed that the inhibitory effect of API@SF nanoparticles on 4T1 cells was significantly higher than that of API raw materials （P＜0.05），and its mechanism may be related to increasing the level of reactive oxygen species in cells. CONCLUSIONS API@SF nanoparticles are prepared successfully ，which possess good safety and anti-tumor activity. |
| 期刊： | 2022年第33卷第01期 |
| 作者： | 季鹏，张进香，王祥龙，葛健文，黄海琴 |
| AUTHORS： | JI Peng，ZHANG Jinxiang，WANG Xianglong，GE Jianwen，HUANG Haiqin |
| 关键字： | 芹菜素；丝素蛋白；纳米粒；制备；安全性；抗肿瘤活性 |
| KEYWORDS： | apigenin；silk fibroin ；nanoparticles；preparation；safety；anti-tumor activity |
| 阅读数： | 376 次 |
| 本月下载数： | 9 次 |
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