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澳洲茄边碱对人肝癌HepG2细胞生长和凋亡的影响及机制初探
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| 篇名： | 澳洲茄边碱对人肝癌HepG2细胞生长和凋亡的影响及机制初探 |
| TITLE： | Preliminary Study on Effects of Solamargine on the Growth and Apoptosis of Human Hepatocarcinoma Cells HepG 2 and Its Mechanism |
| 摘要： | 目的：探讨澳洲茄边碱对人肝癌HepG2细胞生长和凋亡的影响，并研究其潜在作用机制。方法：考察0（空白组）～12μmol/L澳洲茄边碱作用24、48h对HepG2细胞存活率的影响，0（空白组）、6μmol/L澳洲茄边碱作用10d对细胞克隆形成的影响，0（空白组）、4、6、8μmol/L澳洲茄边碱作用24h对细胞凋亡率和细胞中B细胞淋巴瘤2（Bcl-2）、Bcl-2相关X蛋白（Bax）、胱天蛋白酶3（caspase-3）mRNA表达量以及Bcl-2、剪切的caspase-3（cleavedcaspase-3）蛋白表达量和磷酸化腺苷一磷酸活化蛋白激酶α（p-AMPKα）/AMPKα的影响。考察AMPK抑制剂compoundC对经6μmol/L澳洲茄边碱作用24h后细胞中AMPKα、Bcl-2蛋白表达量的影响。结果：与空白组比较，1～12μmol/L澳洲茄边碱作用24、48h均可显著降低细胞存活率（P＜0.05），且有浓度依赖趋势，其半数抑制浓度分别为8.310、7.996μmol/L；经6μmol/L澳洲茄边碱作用10d后细胞的克隆形成率显著降低（P＜0.05）。6、8μmol/L澳洲茄边碱作用后细胞凋亡率和细胞中Bax、caspase-3mRNA及cleavedcaspase-3蛋白（除8μmol/L外）表达量、p-AMPKα/AMPKα（除8μmol/L外）均显著升高（P＜0.05），Bcl-2mRNA及蛋白表达量均显著降低（P＜0.05），部分指标变化有浓度依赖性。compoundC可以抑制AMPKα蛋白表达并逆转澳洲茄边碱对Bcl-2蛋白的抑制作用。结论：澳洲茄边碱可以抑制HepG2细胞增殖，诱导其凋亡，作用机制可能与激活AMPK信号通路有关。 |
| ABSTRACT： | OBJECTIVE：To ex plore the effects of solamargine on the growth and apoptosis of human hepatocarcinoma cells HepG2 and its underlying mechanism. METHODS ：The effects of 0（blank group ）-12 μmol/L solamargine treatment of 24，48 h on survival rate of HepG 2 cells were investigated. The effects of 0（blank group ），6 μmol/L solamargine treatment of 10 days on cell clone formation were also investigated. The effects of 0（blank group ），4，6，8 μmol/L solamargine for 24 h on the apoptotic rate of cells，mRNA expression of Bcl- 2，Bax and caspase- 3， protein expression of Bcl- 2 and cleaved caspase- 3 as well as ratio of p-AMPKα to AMPKα were all tested. The effects of AMPK inhibitor as compound C on the protein expression of AMPKα and Bcl- 2 in cells were investigated after treated with 6 μmol/L solamargine for 24 h. RESULTS ：Compared with 020-39318678。E-mail：wujingjing6028@gzucm.edu.cn blank group ，1-12 μ mol/L solamargine for 24，48 h could significantly decrease the survival rates of cells （P＜0.05）in a concentration-dependent manner ；IC50 of them were 8.310 and 7.996 μmol/L，respectively；the rate of cell clone formation was decreased significantly after treated with 6 μmol/L solamargine for 10 days（P＜0.05）. The apoptotic rate of HepG 2 cells，mRNA expression of Bax and caspase- 3，protein expression of cleaved caspase-3（except for 8 μmol/L）as well as ratio of p-AMPKα to AMPKα（except for 8 μmol/L）were all increased significantly after treated with 6，8 μmol/L solamargine（P＜0.05）；mRNA and protein expression of Bcl- 2 were decreased significantly （P＜ 0.05）；the changes of some indexes were in a concentration-dependent manner. The compound C could inhibit protein expression of AMPKα，and reverse the inhibitory effect of solamargine on Bcl- 2 protein. CONCLUSIONS ：Solamargine can inhibit the proliferation of HepG 2 cells and induce apoptosis ，the mechanism of which may be associated with activating AMPK signaling pathway. |
| 期刊： | 2021年第32卷第24期 |
| 作者： | 吴晶晶，张文娟，张骏鸿，张雅婷，黎莉，卢月，危建安，韩凌 |
| AUTHORS： | WU Jingjing ，ZHANG Wenjuan ，ZHANG Junhong ，ZHANG Yating ，LI Li，LU Yue，WEI Jian ’an，HAN Ling |
| 关键字： | 澳洲茄边碱；人肝癌HepG2细胞；生长；凋亡；磷酸化腺苷一磷酸活化蛋白激酶信号通路 |
| KEYWORDS： | Solamargine；Human hepatocarcinoma cells HepG 2；Growth；Apoptosis；AMPK signaling pathway |
| 阅读数： | 198 次 |
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